LOCUS       sequence-451309-        5824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, 
            McCallion AS, Katsanis N, Reeves RH
  TITLE     Survey of Human Chromosome 21 Gene Expression Effects on Early 
            Development in Danio rerio.
  JOURNAL   G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 
            10.1534/g3.118.200144.
  PUBMED    29760202
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.118"; 
            journalName: "G3 (Bethesda)"; date: "2018-07-2- 2"; volume: "8"; 
            issue: "7"; pages: "2215-2223"
COMMENT     Sequence Label: IFNAR1_pCSdest
FEATURES             Location/Qualifiers
     source          1..5824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..985
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1020..1038
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1020..1037
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    1092..1116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2794..2818)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2862..2996
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2886..2905)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2940..2959
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3109..3129)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3109..3127)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3148..3164)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3148..3164)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3161..3183)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3172..3188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3196..3202,3203..3220,3221..3226))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3241..3262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3379..3396)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3550..4137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3630..3649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4308..5099,5100..5168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4931..4950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5169..5273)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5299..5754)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5436..5457)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5648..5667
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtcgaccgcc aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg
       61 gacctgtgcc aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg
      121 gaccccagcc aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg
      181 gcactgtgcc aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt
      241 ggccctgtgc caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg
      301 gctatatgcc aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta
      361 ttggccaggt tcaatactat gtattggccc tatgccatat agtattccat atatgggttt
      421 tcctattgac gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc
      481 ctaataccgc ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc
      541 tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat
      601 tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg
      661 acgtcaatgg gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca
      721 atgacggtaa atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt
      781 acattactat tggaagtacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg
      841 gtaaatggcc cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat
      901 gacgcaaatg ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta
      961 tataagcaat gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga
     1021 tttaggtgac actatagaat acaagctact tgttcttttt gcaggatccc atcgattcga
     1081 attcaaggat cacaagtttg tacaaaaaag caggcaccat gatggtcgtc ctcctgggcg
     1141 cgacgaccct agtgctcgtc gccgtggcgc catgggtgtt gtccgcagcc gcaggtggaa
     1201 aaaatctaaa atctcctcaa aaagtagagg tcgacatcat agatgacaac tttatcctga
     1261 ggtggaacag gagcgatgag tctgtcggga atgtgacttt ttcattcgat tatcaaaaaa
     1321 ctgggatgga taattggata aaattgtctg ggtgtcagaa tattactagt accaaatgca
     1381 acttttcttc actcaagctg aatgtttatg aagaaattaa attgcgtata agagcagaaa
     1441 aagaaaacac ttcttcatgg tatgaggttg actcatttac accatttcgc aaagctcaga
     1501 ttggtcctcc agaagtacat ttagaagctg aagataaggc aatagtgata cacatctctc
     1561 ctggaacaaa agatagtgtt atgtgggctt tggatggttt aagctttaca tatagcttag
     1621 ttatctggaa aaactcttca ggtgtagaag aaaggattga aaatatttat tccagacata
     1681 aaatttataa actctcacca gagactactt attgtctaaa agttaaagca gcactactta
     1741 cgtcatggaa aattggtgtc tatagtccag tacattgtat aaagaccaca gttgaaaatg
     1801 aactacctcc accagaaaat atagaagtca gtgtccaaaa tcagaactat gttcttaaat
     1861 gggattatac atatgcaaac atgacctttc aagttcagtg gctccacgcc tttttaaaaa
     1921 ggaatcctgg aaaccatttg tataaatgga aacaaatacc tgactgtgaa aatgtcaaaa
     1981 ctacccagtg tgtctttcct caaaacgttt tccaaaaagg aatttacctt ctccgcgtac
     2041 aagcatctga tggaaataac acatcttttt ggtctgaaga gataaagttt gatactgaaa
     2101 tacaagcttt cctacttcct ccagtcttta acattagatc ccttagtgat tcattccata
     2161 tctatatcgg tgctccaaaa cagtctggaa acacgcctgt gatccaggat tatccactga
     2221 tttatgaaat tattttttgg gaaaacactt caaatgctga gagaaaaatt atcgagaaaa
     2281 aaactgatgt tacagttcct aatttgaaac cactgactgt atattgtgtg aaagccagag
     2341 cacacaccat ggatgaaaag ctgaataaaa gcagtgtttt tagtgacgct gtatgtgaga
     2401 aaacaaaacc aggaaatacc tctaaaattt ggcttatagt tggaatttgt attgcattat
     2461 ttgctctccc gtttgtcatt tatgctgcga aagtcttctt gagatgcatc aattatgtct
     2521 tctttccatc acttaaacct tcttccagta tagatgagta tttctctgaa cagccattga
     2581 agaatcttct gctttcaact tctgaggaac aaatcgaaaa atgtttcata attgaaaata
     2641 taagcacaat tgctacagta gaagaaacta atcaaactga tgaagatcat aaaaaataca
     2701 gttcccaaac tagccaagat tcaggaaatt attctaatga agatgaaagc gaaagtaaaa
     2761 caagtgaaga actacagcag gactttgtat gaaacccagc tttcttgtac aaagtggtga
     2821 tcctctcgag cctctagaac tatagtgagt cgtattacgt agatccagac atgataagat
     2881 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2941 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     3001 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat
     3061 tcgcggccgc ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg
     3121 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3181 gctcacaatt ccacacaaca tacgagccgg gagcataaag tgtaaagcct ggggtgccta
     3241 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3301 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3361 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3421 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3481 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3541 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3601 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3661 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3721 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3781 cgttcgctcc aagctgggct gtgtgcacga acccccgttc agcccgaccg ctgcgcctta
     3841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3961 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4441 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5281 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     5341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     5401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     5461 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     5521 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     5581 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     5641 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     5701 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     5761 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5821 gcca
//
