LOCUS       sequence-45134-e        8524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Astrakhan A, Sather BD, Ryu BY, Khim S, Singh S, Humblet-Baron S, 
            Ochs HD, Miao CH, Rawlings DJ
  TITLE     Ubiquitous high-level gene expression in hematopoietic lineages 
            provides effective lentiviral gene therapy of murine Wiskott-Aldrich
            Syndrome.
  JOURNAL   Blood. 2012 Mar 19.
  PUBMED    22431569
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2012
            Mar 19."
COMMENT     Sequence Label: pRRL WS1.6 GFP
FEATURES             Location/Qualifiers
     source          1..8524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3640..3649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3646..3648,3649..3651,3652..4365)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3646..3648,3649..3651,3652..4362)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3691..3712)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3952..3971)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4299..4320
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4378..4966
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4431..4451)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4849..4860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5053..5286
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5358..5479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5395..5414)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5449..5468
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5498..5633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5665..5684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5666..5684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5694..5711)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5694..5710)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5703..5725)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5855..6310
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5942..5961)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6152..6173
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6339..6443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6444..6512,6513..7304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6662..6681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7475..8063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7964..7983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8217..8234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8351..8372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8387..8392,8393..8410,8411..8417)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8425..8441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8430..8452
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8449..8465
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8449..8465
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8484..8504
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8486..8504
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattcccac ggggttggac
     1981 gcgttgcagt gttgctccca acagcaagaa ccagaagcag cccaaagggc tgttacagga
     2041 gaatatggac acccaggctg cacatgcaca ccatggaatg ctgtatggca gtggaaataa
     2101 atgaacagct accactatag gcaaacagga atcacagcaa cagccaagag tgaaggcgtg
     2161 gagggaccga gaccatgcac tcacacctgg cctgcctggc tcgcactccg ggcaaagggg
     2221 tcagaacagt gactggcaca cacgttaagt gctatgtgag tgttaagata aaactaggat
     2281 gtccagtggg gagaaagcaa gcctttgaag attatgtgct tttacaaact tcaagtgcaa
     2341 tgaaaactaa acaagatgtt gttcaggcat tcatatatga tataaagttc ctttctttaa
     2401 aaaagggatg ggctgggcac ggtggctcac gcctgtaatt ctaatacttt gggaggccga
     2461 ggcaggtgga tcacgaggtc gagaaatcga gaccatcctg gccaacatgg tgaaaccctg
     2521 tctctactaa aaatacaaaa aaattagctg ggcgtggtgg cgtgtgcctg tagtcccagc
     2581 tacttgggag gctgaggcag gagagtcact tgaacccggg aggcaaaggt tgcagtgagc
     2641 cgagatcgtg ccacccgcac tccagcctgg cgacagagtg agactccatc tcaaaaaaaa
     2701 aaagaaaaaa aaaagtatga caagcagaaa gtaatttggg agctgcgggg aggcaagggt
     2761 aagggatggg gaagtggacc agaggcatat gcgtcattgg cagtgtctaa gcactcacga
     2821 taggcgtgga tcacaggggc tcgctctgta attaaaagga aaagggtttt tgttgtgttg
     2881 ttgttgttgc tgtttttgag acaagggtct tgctctgtca tcatccaggc tggagtgcag
     2941 tggtgcagtc tcagctcact gcaacctccg cctcctgggt tcaagcgatt ctcctgcctc
     3001 agcctcctga gcagctagga ctacaggtgt gtgccaccat gcctggctaa tttttgtatt
     3061 ttttagtgga aatggggttt tgccatgttg cccaggctcg tcttgaactc ctgacctcaa
     3121 gtgatccact cgtctcggcc tcccaaagtg ctgggattac aggtgtgagc tattgtcccc
     3181 agccaaaagg aaaagtttta ctgtagtaac ccttccggac tagggacctc gggcctcagc
     3241 ctcaggctac ctaggtgctt tagaaaggag gccacccagg cccatgacta ctccttgcca
     3301 cagggagccc cgcacacaga tgtgctaagc tctcgctgcc agccagaggg aggagggtct
     3361 gagccagtca gaaggagatg ggccccagag agtaagaaag ggggaggagg acccaagctg
     3421 atccaaaagg tgggtctaag cagtcaagtg gaggagggtt ccaatctgat ggcggagggc
     3481 ccaagctcag cctaacgagg aggccaggcc caccaagggg cccctggagg acttgtttcc
     3541 cttgtccctt gtggtttttt gcatttcctg ttcccttgct gctcattgcg gaagttcctc
     3601 ttcttaccct gcacccagag cctcgccagg atcctcgagg ccaccatggt gagcaagggc
     3661 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3721 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3781 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3841 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3901 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3961 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4021 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4081 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     4141 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4201 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4261 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4321 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaactag tgtcgacaat
     4381 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4441 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4501 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4561 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4621 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4681 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4741 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4801 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4861 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4921 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct
     4981 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5041 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5101 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5161 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5221 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5281 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5341 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5401 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5461 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     5521 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5581 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5641 ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     5701 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5761 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5821 caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc
     5881 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5941 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6001 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6061 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6121 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6181 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6241 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6301 tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6361 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6421 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6481 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6541 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6601 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6661 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6721 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6781 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6841 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6901 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6961 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7021 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7081 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7141 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7201 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7261 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7621 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8161 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8221 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8281 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8341 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8401 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8461 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     8521 tgca
//
