LOCUS       sequence-451366-        4520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion 
            leaving an Ery resistance cassette flanked by BxbI recombination 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-443_Cacnes_HisB
FEATURES             Location/Qualifiers
     source          1..4520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    750..807
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(1458..2046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1538..1557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2129..2920,2921..2989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2990..3094)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    3744..3792
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 atttgcccgt aagcgcaccg gtgattatgg ccgctggtcc cgcctcacgg tcatgacttg
       61 gccgctcttc gagtgggagt tcgtcgagaa ggtcgatcgc cgcttgttca agccggtccc
      121 gaaggtcgat tccgccatca tgcgtctgcg tcgccgcgct gaaccgctgt tggagggtgc
      181 tgcccttgag cgctacgaga gcatggtgga gttgtgtttt actggtgtcg gcggcaacat
      241 tcaagcctcc ctcctgcgca agtaccctcg tcgtcgcgtg gaggctgcgc tggaccatgc
      301 cggcgtgggt ggtggtgctg tcgtggccta cgtccggccg gagcaatggc tgcggctctt
      361 cgagcgcctt gaccagaaga acgagccgcg cggtggccaa ccgcagcgcg gccgtcgtac
      421 gggtggtcag gatcgtggtg accgccgtac gggtggccag gatcgtggcg accgtcggac
      481 gggtggtcag gaccgtcgcg accgtcaggc ctccggccac ggtgaccgtc ggtcctctgg
      541 ccgtaatcgc gacgatggtc gtaccgggga gcgcgagcag ggcgaccagg gtggtcgtcg
      601 tggcccttcg ggtggtggtc gtaccggcgg tcgtccaggc cgtcgtggcg gtccgggtca
      661 gcgctgatct agacccgcac ttaacccgct tcggcgggtt tttgtttttt ggggtgcagg
      721 taggccatat tgtcttcggg gccccactcg tcgtggtttg tctggtcaac caccgcggtc
      781 tcagtggtgt acggtacaaa ccccgacgag gcgataagct cggcaaggct gattacgcct
      841 gctcgtggaa ttctagaact ctgtgaggat aaagtctccc ttacagcctg cggtccgggg
      901 tgcgggtcgg cattatcgac catggatcag gaaaccttca ctccgctaga cgtgcgttgc
      961 gtcaggtagg tgccgaggtg atcgtttcca acgacccgag tgccctactc gataccgacg
     1021 ctctcgtcct ccccggagtg ggagcgttag cggtgtgtat gacagggttg cgggctatgg
     1081 gcggggcccg tcttgtccgt caatgggttg acgagggacg acctctgctg ggtatctgcg
     1141 tgggccacca gatgcttttc gagcgaggac gtgaacgcga cgttgacgtc aagtgccttg
     1201 gtgttcttcc tggcgtcgtt gaggagctgc cagcagagcg actgccccat atggggtgga
     1261 acacagtgac accggccacc gactcagcct tattccaggg cgtgcaagag aggttctact
     1321 tcgtccattc ctatggtgtg gtcgttaccg gacctcacga ccacttcacc actgcgaccc
     1381 atcagggagc caccttcgct gggacgcccg ctaggctggc ggggttgcct tactgatcag
     1441 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1861 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     1921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2101 cgttaaggga ttttggtcat gaggcgcctc accaatgctt gatgagggag gcaccgatct
     2161 cggcgatctg acggttgcgc tcatccatcg tggcctggga gccggtcgtg tagatcacca
     2221 cgatgcggct gggcttaccg tcgggaccga gagccgcgat gatgccacgc gagccgcgct
     2281 caccagcacc cgacttgtcc gcgatgaacc aaccggccgg gagcgcgctg cgcaacagcg
     2341 ggccagcgac cttgtcggcc tccatccagt cgatcagttg ctgacgggag gccagagtga
     2401 ggagttcacc ggtcaggagt ttacggaggg tggtagccat agccgcgggc atagtcgtgt
     2461 cgcgctcgtc gttcgggata gcctcattaa gttcgggttc ccaacggtcc agacgggtca
     2521 cgtggtcgcc catgttgtgg agaaaagccg tcagctcttt cgggccaccg atggtcgtca
     2581 acagcagatt ggcggcagtg ttgtcggaca tagtgatggc cgcggagcac agctcgcgca
     2641 cggtcatccc atcggtcaga tgtttctccg tgaccgggga gtattcgacc aggtcgttct
     2701 gggagtaatg aatgcgacgg cccagttgct cctgcccagc atccacccgg ctaagcacag
     2761 cgccgcagag gaggaccttg aacgtggaca tcatggggaa acgctcctca gggcggaaag
     2821 actccagaat cttaccggag ttcaggtcca actcgatata accgacgcgc gcccccagct
     2881 gatcctcggc gtccttgacc ttgaccaggg tttccggatg ggcaaaaacc ggcaagcaaa
     2941 aggccgcaaa aaatgggata agggcaacgc ggaagtgctg gatcgacata ctcttccttt
     3001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3061 gtatttagaa aaataaacaa ataggggttc cgcgactagt tccccgaaaa gtgccacctg
     3121 acgttaagaa accattatta cagcctgcgg tccgggactc ctcgattgct ttgcttccga
     3181 agtacggtcg gctcattgtg gtgcgcaccc tatccaaggc cttcgccctg gcgggtggcc
     3241 gactggggta tgcggtggcc gcaccggccg tcgttgacgc gctgcagatg gtgcggctgc
     3301 cctatcatct ctcggacgtg tcacaggtgg tggcgagagt tgccctcgct catgcccccg
     3361 agatgcttgc gcgggtcgac gagttgcgtg agacccgcgc aaacctggag gactggctgc
     3421 aactgcacgg gttggacgtc gtttcctcac agtcgaactt cgtcctcttc ggcaggttcg
     3481 ctgatcgtca cgcggtattc caggcgctgc ttgatcgcgg tgtgctcatc cgagaggtcg
     3541 gaccaactgg ctacttgcgc gtctgcgccg gaaccccatg ccagaccgac gctttccgta
     3601 ccgctttgct tgacgtcctt cccaccgctc cgcgtctcgt cccggaggag gatcatcgct
     3661 gggacgcccg gtagtgctta tcagacccaa tactgttgaa aagcttgcgc caaccatgca
     3721 ccatagccag cctgacagta gcccggccgg cttgtcgacg acggcggtct ccgtcgtcag
     3781 gatcatccgg gcgcagctga gagcgacaca gtcctgtact tgggatgctg gcacaccgac
     3841 gatctgcacg ggcgagtcta ggggttatcg gctacatgcg cgtcgccgca tcaggttcac
     3901 gaaaccgaaa ggcagaccca ccccctaccg ggcataccgt tgccgccgac acttcgatga
     3961 cactatgcag tgctacactt caactgaaaa tctatatgat tttcgtcaat tcccatacgt
     4021 acgacacatc gggagatcgt catgtcgtcg tccgacgagc aaccgcgccc gcgtcgccgc
     4081 aaccaggacc gccagcaccc gaaccagaac cgcccggtcc tgggccgtac tgagcgggac
     4141 cgcaaccgcc gtcaattcgg tcagaatttc cttcgtgacc gtaagacgat cgcccgcatc
     4201 gcggaaacgg ccgaactgcg ccccgacctt ccggtcctgg aggctggccc aggcgagggt
     4261 ctgctgaccc gtgagcttgc cgatcgggcc cgccaggtga cgagctacga gatcgacccg
     4321 cgcttggcga agtccctgcg tgagaagctg tccggccacc cgaatatcga ggtcgtcaac
     4381 gcggacttcc tgaccgccga gccccctccc gagccgtttg ccttcgtcgg cgcgatcccg
     4441 tacggcatca cgtctgcgat cgtcgattgg tgcctggagg ctccgacgat cgaaaccgcc
     4501 acgatggtta cccaactcga
//
