LOCUS       sequence-451370-        4523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion 
            leaving an Ery resistance cassette flanked by BxbI recombination 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-446_Cacnes_ProA
FEATURES             Location/Qualifiers
     source          1..4523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1066..1123
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(1777..2365)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1857..1876)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2448..3239,3240..3308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3309..3413)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    4063..4111
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 aaacggccga actgcgcccc gaccttccgg tcctggaggc tggcccaggc gagggtctgc
       61 tgacccgtga gcttgccgat cgggcccgcc aggtgacgag ctacgagatc gacccgcgct
      121 tggcgaagtc cctgcgtgag aagctgtccg gccacccgaa tatcgaggtc gtcaacgcgg
      181 acttcctgac cgccgagccc cctcccgagc cgtttgcctt cgtcggcgcg atcccgtacg
      241 gcatcacgtc tgcgatcgtc gattggtgcc tggaggctcc gacgatcgaa accgccacga
      301 tggttaccca actcgaattt gcccgtaagc gcaccggtga ttatggccgc tggtcccgcc
      361 tcacggtcat gacttggccg ctcttcgagt gggagttcgt cgagaaggtc gatcgccgct
      421 tgttcaagcc ggtcccgaag gtcgattccg ccatcatgcg tctgcgtcgc cgcgctgaac
      481 cgctgttgga gggtgctgcc cttgagcgct acgagagcat ggtggagttg tgttttactg
      541 gtgtcggcgg caacattcaa gcctccctcc tgcgcaagta ccctcgtcgt cgcgtggagg
      601 ctgcgctgga ccatgccggc gtgggtggtg gtgctgtcgt ggcctacgtc cggccggagc
      661 aatggctgcg gctcttcgag cgccttgacc agaagaacga gccgcgcggt ggccaaccgc
      721 agcgcggccg tcgtacgggt ggtcaggatc gtggtgaccg ccgtacgggt ggccaggatc
      781 gtggcgaccg tcggacgggt ggtcaggacc gtcgcgaccg tcaggcctcc ggccacggtg
      841 accgtcggtc ctctggccgt aatcgcgacg atggtcgtac cggggagcgc gagcagggcg
      901 accagggtgg tcgtcgtggc ccttcgggtg gtggtcgtac cggcggtcgt ccaggccgtc
      961 gtggcggtcc gggtcagcgc tgatctagac ccgcacttaa cccgcttcgg cgggtttttg
     1021 ttttttgggg tgcaggtagg ccatattgtc ttcggggccc cactcgtcgt ggtttgtctg
     1081 gtcaaccacc gcggtctcag tggtgtacgg tacaaacccc gacgaggcga taagctcggc
     1141 aaggctgatt acgcctgctc gtggaattct agaactctgt gaggataaag tctcccttac
     1201 agcctgcggt ccggccgttt aacggttgtc gtggggcggt tgtgggatag gctgagacgt
     1261 catgagtatc gttcctctcc tgatggagtc gtcggccctc gtcgtcggca tcttgacgct
     1321 gctggttctg ctgatcgcgt tggctatcgt ccgtggtatc ggcaagggtc ggccgcattc
     1381 ctgatgccaa gtgttgagct ggggctggcg cgggtccaca acggacgccg ctaccggctc
     1441 ggggtgatgg gtggcacctt cgaccccatt catcatggcc acctcgtcgc agcctcggag
     1501 gttgcggcca gattcgacct cgacgaggtc gttttcgttc ccaccggcgt gccttggcaa
     1561 aagaagggac gcagggtttc gcaggccgag gatcgctacc tcatgaccgt catcgcgacg
     1621 gcgtcgaatc cgtctttttc ggtgtcgcgg gtcgatatcg atcgtccggg ggacacgtac
     1681 accgtcgaca ccctcaagga tctgcgccgg gagctcgctg ggacgcccgc taggctggcg
     1741 gggttgcctt actgatcagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2401 tcagtggaac gaaaactcac gttaagggat tttggtcatg aggcgcctca ccaatgcttg
     2461 atgagggagg caccgatctc ggcgatctga cggttgcgct catccatcgt ggcctgggag
     2521 ccggtcgtgt agatcaccac gatgcggctg ggcttaccgt cgggaccgag agccgcgatg
     2581 atgccacgcg agccgcgctc accagcaccc gacttgtccg cgatgaacca accggccggg
     2641 agcgcgctgc gcaacagcgg gccagcgacc ttgtcggcct ccatccagtc gatcagttgc
     2701 tgacgggagg ccagagtgag gagttcaccg gtcaggagtt tacggagggt ggtagccata
     2761 gccgcgggca tagtcgtgtc gcgctcgtcg ttcgggatag cctcattaag ttcgggttcc
     2821 caacggtcca gacgggtcac gtggtcgccc atgttgtgga gaaaagccgt cagctctttc
     2881 gggccaccga tggtcgtcaa cagcagattg gcggcagtgt tgtcggacat agtgatggcc
     2941 gcggagcaca gctcgcgcac ggtcatccca tcggtcagat gtttctccgt gaccggggag
     3001 tattcgacca ggtcgttctg ggagtaatga atgcgacggc ccagttgctc ctgcccagca
     3061 tccacccggc taagcacagc gccgcagagg aggaccttga acgtggacat catggggaaa
     3121 cgctcctcag ggcggaaaga ctccagaatc ttaccggagt tcaggtccaa ctcgatataa
     3181 ccgacgcgcg cccccagctg atcctcggcg tccttgacct tgaccagggt ttccggatgg
     3241 gcaaaaaccg gcaagcaaaa ggccgcaaaa aatgggataa gggcaacgcg gaagtgctgg
     3301 atcgacatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgactagtt
     3421 ccccgaaaag tgccacctga cgttaagaaa ccattattac agcctgcggt ccggtggatg
     3481 ccccggacgt tctggctggt gtccccgtgg gtacctactt ccgcccgctg gcgacgcgac
     3541 ggccccgacg gttgctgtgg ttggccgacg ctgccacccc gcagggacag atcgtcatcg
     3601 acgacggagc tgtcgaagct ttgacacagc gtcattcctc gttgttggcg gtgggtgtga
     3661 ctcgggtaca cggggatttc caagcaggcg acccagtgac gatcctggcc tccgacggtc
     3721 gagttgttgg tcgcggtatc gcccagttct cccatgatga ggtgcgcgtc atgaggggac
     3781 gttcgagtgc ctggctggcc gcagaaatgg gtccggcggc ctctcgcgag atcatccacc
     3841 gcgatgccat ggtgctgagt cgtcgtcgta aagctgagcc gtcgtcgagg aaccagaaaa
     3901 gtagcgggtc aagggtgacg tcctgactca cggtctggtt atcaggtgag tgatgctcag
     3961 ctgacgaata ccattcgctg ggacgcccgg tagtgcttat cagacccaat actgttgaaa
     4021 agcttgcgcc aaccatgcac catagccagc ctgacagtag cccggccggc ttgtcgacga
     4081 cggcggtctc cgtcgtcagg atcatccggg cgcagctgag agcgacacag tcctgtactt
     4141 gggatgctgg cacaccgacg atctgcacgg gcgagtctag gggttatcgg ctacatgcgc
     4201 gtcgccgcat caggttcacg aaaccgaaag gcagacccac cccctaccgg gcataccgtt
     4261 gccgccgaca cttcgatgac actatgcagt gctacacttc aactgaaaat ctatatgatt
     4321 ttcgtcaatt cccatacgta cgacacatcg ggagatcgtc atgtcgtcgt ccgacgagca
     4381 accgcgcccg cgtcgccgca accaggaccg ccagcacccg aaccagaacc gcccggtcct
     4441 gggccgtact gagcgggacc gcaaccgccg tcaattcggt cagaatttcc ttcgtgaccg
     4501 taagacgatc gcccgcatcg cgg
//
