LOCUS       sequence-451372-        6029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cutibacterium acnes replicative plasmid for recombinant expression. 
            pBRESP36A-based vector optimized for reduced size and modular 
            assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-350_Cacnes_ori
FEATURES             Location/Qualifiers
     source          1..6029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2939..3527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3019..3038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3610..4401,4402..4470))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4471..4575)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4642..4660
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctagaggaa atcagagctt gaggctgccg gagcgacggt agacccgtcc ggttgggtgg
       61 tgccactgcg tgcactcaag gtcgtttttg gggtgtcaga tgtgacctcg aatgcggaat
      121 tcataggata gattctggaa actttaccgt ccgagctcag gcttacctta ctcgagcaat
      181 aaacagttga tagggcttct ccgttaccat ggttcagcca aaaaacttaa gaccgccggt
      241 cttgtccact accttgcagt aatgcggtgg acaggatcgg cggttttctt ttctcttctc
      301 aattcttctg acctgtaacg aataatagat agtaaagtag tctccgattg agttttctct
      361 gccgagtccc acccagttct gtgatttcag taagttggta attgatacac tgttgcgaga
      421 actggtgcct ggtagtagat aggttgttat tgagtaagaa ggtaaagtga acgaaatccc
      481 tgaaactgag actgtagaaa ataagcttgt ccagactatt ggatccaaga gatttctaca
      541 cgattgagca ctgtctcctg caggctcggt accaaattcc agaaaagagg cctcccgaaa
      601 ggggggcctt ttttcgtttt ggtcctaata gataaaggat aggtctggta gtgttgttcg
      661 ttctcgcagg taaatcaata atactctgca gttccgtaga cttttcagtg ggacagggta
      721 gcgataacag atagattgta ataagacaca gtaggtgctc gtagttgcgt gaagagaacc
      781 gctcaggaaa tccagtcaga agtattggta atcgttgaaa actcagtcga cgcacttact
      841 gaagacgtcc tattacactc gtcgttggaa actgaagatg cggccgcgga aacacagaaa
      901 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggtagcga acgacgagtc
      961 actgttgagg ataaatactt tctctactag ctagctgtta cacaggtcct cagcggcgcg
     1021 cccgcccggt catgacgctg agttagtggc gcagctgcgc tctgagaacg agtttcgacc
     1081 ctgacagccg gcgagctgtt caggcgaaga tcgcacagct gcgcgaggaa ctagccgcaa
     1141 tgcccgaaca cgccccagcc atcccttgga gcaggtggca gcgtcagggg agtcggggga
     1201 tgtttggcag gggatgtgga aagagagttc gctttgctca catggctcaa ccgggtaact
     1261 aactgatatg ggctcttcgt cgcccacttt gaacacgccg aggaatggac cacgctgaac
     1321 gtgactcgca tgcttcactg catgtatgga ttcgttcgag actttgttcc ctgagagctg
     1381 gctgccacgc aagccgctgg cgtcagccga gaagtctggg gcgtaccggc acgtgactcg
     1441 gcagagggcg ctggagctgc cttacatcga agcgaacccg ttggtcatgc agtccttggt
     1501 catcaccgat cgagatgctt cggatgctga ctgggccgca gacctcgctg ggctgccttc
     1561 accgtcctac gtgtccatga accgtgtcac gaccaccgga cacatcgtct atgccttgaa
     1621 gaaccctgtg tgtctgaccg atgccgcgcg gcgacggcct atcaacctgc tcgcccgcgt
     1681 cgagcagggc ctatgcgacg ttctcggcgg cgatgcatcc tacgggcacc ggatcacaaa
     1741 gaacccgctc agcaccgccc atgcgaccct ctggggcccc gcagacgcgc tctacgagct
     1801 gcgcgccctc gcacacaccc tcgacgagat ccacgcactg ccggaggcag ggaacccgcg
     1861 tcgcaacgtc acccgatcaa cggtcggccg caacgtcacc ctgttcgaca ccacccgcat
     1921 gtgggcatac cgggccgtcc ggcactcctg gggcggcccg gtcgccgaat gggagcacac
     1981 cgtattcgag cacatccacc tactgaacga aacgatcatc gccgacgagt tcgccacagg
     2041 ccccctcggc ttgaacgaac ttaagcactt atctcgatcc atttcccgat gggtctggcg
     2101 caacttcacc cccgaaacct tccgcgcacg ccagaaagcg atcagcctcc gtggagcatc
     2161 caaaggcggc aaagaaggcg gccacaaagg cggcattgcc agtggcgcat cacggcgtgc
     2221 ccatacccgt caacagttct tggagggtct gtcatgacca cacgtgaacg cctcccccgc
     2281 aacggctaca gcatcgccgc cgctgcgaaa aagctcggtg tctccgagtc caccgtcaag
     2341 cggtggactt ccgagccacg cgaggagttc gtggcccgcg ttgccgcacg ccacgcgcgg
     2401 attcgtgagc tgcgctcgga gggtcagagc atgcgtgcga ttgctgccga ggtcggggtt
     2461 tccgtgggca ccgtgcacta cgcgctgaac aagaatcgaa ctgacgcatg accgtaacgc
     2521 cgcacgatga gcattttctt gatcgtgcac cgcttggcac tacgttcgcg tgcggttgca
     2581 cagtgcgcgc cacgttctta tcctgcggcc attgtggcta cagccaatgg ggggcatcag
     2641 caacggacgt tgaacccggt gggcaagtgt tactcagggg gacatgccca gtctgcggcg
     2701 ctcggattga cggtatggca gtcgtgcatg cggccccacc gtcaaactca ttcaggtatc
     2761 agtgagaacc ctcatggcac cccctcgtga cacgttctcg ttgcgatcag ctgcggccaa
     2821 gctgatcttc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     2881 tgaggacccg gctaggctgg cggggttgcc ttactgatca ggccgcgttg ctggcgtttt
     2941 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3001 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3061 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3121 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3181 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3241 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3301 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3361 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3421 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3481 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3541 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3601 tgaggcgcct caccaatgct tgatgaggga ggcaccgatc tcggcgatct gacggttgcg
     3661 ctcatccatc gtggcctggg agccggtcgt gtagatcacc acgatgcggc tgggcttacc
     3721 gtcgggaccg agagccgcga tgatgccacg cgagccgcgc tcaccagcac ccgacttgtc
     3781 cgcgatgaac caaccggccg ggagcgcgct gcgcaacagc gggccagcga ccttgtcggc
     3841 ctccatccag tcgatcagtt gctgacggga ggccagagtg aggagttcac cggtcaggag
     3901 tttacggagg gtggtagcca tagccgcggg catagtcgtg tcgcgctcgt cgttcgggat
     3961 agcctcatta agttcgggtt cccaacggtc cagacgggtc acgtggtcgc ccatgttgtg
     4021 gagaaaagcc gtcagctctt tcgggccacc gatggtcgtc aacagcagat tggcggcagt
     4081 gttgtcggac atagtgatgg ccgcggagca cagctcgcgc acggtcatcc catcggtcag
     4141 atgtttctcc gtgaccgggg agtattcgac caggtcgttc tgggagtaat gaatgcgacg
     4201 gcccagttgc tcctgcccag catccacccg gctaagcaca gcgccgcaga ggaggacctt
     4261 gaacgtggac atcatgggga aacgctcctc agggcggaaa gactccagaa tcttaccgga
     4321 gttcaggtcc aactcgatat aaccgacgcg cgcccccagc tgatcctcgg cgtccttgac
     4381 cttgaccagg gtttccggat gggcaaaaac cggcaagcaa aaggccgcaa aaaatgggat
     4441 aagggcaacg cggaagtgct ggatcgacat actcttcctt tttcaatatt attgaagcat
     4501 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4561 aataggggtt ccgcgactag ttccccgaaa agtgccacct gacgttaaga aaccattatt
     4621 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct acaatcagcg
     4681 ctgacccgga ccgccacgac ggcctggacg accgccggta cgaccaccac ccgaagggcc
     4741 acgacgacca ccctggtcgc cctgctcgcg ctccccggta cgaccatcgt cgcgattacg
     4801 gccagaggac cgacggtcac cgtggccgga ggcctgacgg tcgcgacggt cctgaccacc
     4861 cgtccgacgg tcgccacgat cctggccacc cgtacggcgg tcaccacgat cctgaccacc
     4921 cgtacgacgg ccgcgctgcg gttggccacc gcgcggctcg ttcttctggt caaggcgctc
     4981 gaagagccgc agccattgct ccggccggac gtaggccacg acagcaccac cacccacgcc
     5041 ggcatggtcc agcgcagcct ccacgcgacg acgagggtac ttgcgcagga gggaggcttg
     5101 aatgttgccg ccgacaccag taaaacacaa ctccaccatg ctctcgtagc gctcaagggc
     5161 agcaccctcc aacagcggtt cagcgcggcg acgcagacgc atgatggcgg aatcgacctt
     5221 cgggaccggc ttgaacaagc ggcgatcgac cttctcgacg aactcccact cgaagagcgg
     5281 ccaagtcatg accgtgaggc gggaccagcg gccataatca ccggtgcgct tacgggcaaa
     5341 ttcgagttgg gtaaccatcg tggcggtttc gatcgtcgga gcctccaggc accaatcgac
     5401 gatcgcagac gtgatgccgt acgggatcgc gccgacgaag gcaaacggct cgggaggggg
     5461 ctcggcggtc aggaagtccg cgttgacgac ctcgatattc gggtggccgg acagcttctc
     5521 acgcagggac ttcgccaagc gcgggtcgat ctcgtagctc gtcacctggc gggcccgatc
     5581 ggcaagctca cgggtcagca gaccctcgcc tgggccagcc tccaggaccg gaaggtcggg
     5641 gcgcagttcg gccgtttccg cgatgcgggc gatcgtctta cggtcacgaa ggaaattctg
     5701 accgaattga cggcggttgc ggtcccgctc agtacggccc aggaccgggc ggttctggtt
     5761 cgggtgctgg cggtcctggt tgcggcgacg cgggcgcggt tgctcgtcgg acgacgacat
     5821 gacgatctcc cgatgtgtcg tacgtatggg aattgacgaa aatcatatag attttcagtt
     5881 gaagtgtagc actgcatagt gtcatcgaag tgtcggcggc aacggtatgc ccggtagggg
     5941 gtgggtctgc ctttcggttt cgtgaacctg atgcggcgac gcgcatgtag ccgataaccc
     6001 ctagactcgc ccgtgcagat cgtcggtgt
//
