LOCUS       sequence-451376-        6059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cutibacterium acnes replicative plasmid for recombinant expression. 
            pBRESP36A-based vector with C. acnes chloramphenicol resistance, 
            harbouring a medium-copy pBR322 E. coli ori (no ROP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-349_Cacnes_ori
FEATURES             Location/Qualifiers
     source          1..6059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(551..655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     722..740
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(5078..5666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5158..5177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5749..6059,1..481,482..550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tagccgcggg catagtcgtg tcgcgctcgt cgttcgggat agcctcatta agttcgggtt
       61 cccaacggtc cagacgggtc acgtggtcgc ccatgttgtg gagaaaagcc gtcagctctt
      121 tcgggccacc gatggtcgtc aacagcagat tggcggcagt gttgtcggac atagtgatgg
      181 ccgcggagca cagctcgcgc acggtcatcc catcggtcag atgtttctcc gtgaccgggg
      241 agtattcgac caggtcgttc tgggagtaat gaatgcgacg gcccagttgc tcctgcccag
      301 catccacccg gctaagcaca gcgccgcaga ggaggacctt gaacgtggac atcatgggga
      361 aacgctcctc agggcggaaa gactccagaa tcttaccgga gttcaggtcc aactcgatat
      421 aaccgacgcg cgcccccagc tgatcctcgg cgtccttgac cttgaccagg gtttccggat
      481 gggcaaaaac cggcaagcaa aaggccgcaa aaaatgggat aagggcaacg cggaagtgct
      541 ggatcgacat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      601 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgactag
      661 ttccccgaaa agtgccacct gacgttaaga aaccattatt atcatgacat taacctatcg
      721 gaataggcgt atcacgaggc cctttcgtct acaatcagtt agcctcggcg gcagtcttag
      781 tcagagcgcg gcgcgtgagg agcatgatca ccagggcgat ggcggtcagg accgaagcga
      841 cccagaccgg agccagcagg cccaggccag tagccaggcc cagagcgccc agcaccggac
      901 cggcggcagc gccgatgttc agggcggcgg tggcgtagct accgcccata gtcggagcac
      961 cagaggccgc gtacaggacg cgcgtaatga gggtggaacc gacgccaaag gacaggaagc
     1021 cctggacgag aaccaggacg atcagggcga ccgggtggga ggccaccacg gccaggacga
     1081 tccagccggt aagcaacagc ggcccgccca ccgccaggac caggcccgga cgctggtcgg
     1141 acagacgacc ggcaatagtg acgcccagga acgagccgat accgaacatg accagggcga
     1201 ccgagaccca agcctcggcc aggccggcgg tctcggtgac gattggggcc aggaaggtga
     1261 aggccgcgaa ggtcccgccg ttgatgagag cacccagggc catagccaga ataaggcgtg
     1321 gggtggcgag ctgggacagc tcgacgcgca ggcgagggga cgtggcggag gtctcggaac
     1381 ggccgacgtt gttggtgacg ccgcggatga cgccgacggc ggcggggata cacaggatcg
     1441 cgatagccca aaaggtggtc cgccacccca gggcggtgcc caggagagcg ccggccggga
     1501 caccgacgac ggtggcaatg gtggtaccag agagaaggat ggagagggca cggcccttct
     1561 ggttggctgg gaccaacgtc gtggcggtag acagcgcgac agccagaaag cccgcgttcg
     1621 ccagggccga aaggacgcga gtaatgagca ggagcgaaaa gaccggggtc atagcaccga
     1681 taacatggga gccggcgaaa accaacaggc acacgatcag agtcaggcgc gggggccagc
     1741 ggcgggcaaa ggcggccatg acaggagcgc ccacgaccat gccaacggca aaggcggaag
     1801 tcagcagacc cgcagtgccc acgctaacgt caagctccgt ggcgatagcc ggaagcaggc
     1861 cggccaacat gaactcgctg gtgcccatga caaacacggc gagggccagc atgtacaggg
     1921 cgaagggcat gacgatctcc cgatgtgtcg tacgtatggg aattgacgaa aatcatatag
     1981 attttcagtt gaagtgtagc actgcatagt gtcatcgaag tgtcggcggc aacggtatgc
     2041 ccggtagggg gtgggtctgc ctttcggttt cgtgaacctg atgcggcgac gcgcatgtag
     2101 ccgataaccc ctagactcgc ccgtgcagat cgtcggtgtt ctagaggaaa tcagagcttg
     2161 aggctgccgg agcgacggta gacccgtccg gttgggtggt gccactgcgt gcactcaagg
     2221 tcgtttttgg ggtgtcagat gtgacctcga atgcggaatt cataggatag attctggaaa
     2281 ctttaccgtc cgagctcagg cttaccttac tcgagcaata aacagttgat agggcttctc
     2341 cgttaccatg gttcagccaa aaaacttaag accgccggtc ttgtccacta ccttgcagta
     2401 atgcggtgga caggatcggc ggttttcttt tctcttctca attcttctga cctgtaacga
     2461 ataatagata gtaaagtagt ctccgattga gttttctctg ccgagtccca cccagttctg
     2521 tgatttcagt aagttggtaa ttgatacact gttgcgagaa ctggtgcctg gtagtagata
     2581 ggttgttatt gagtaagaag gtaaagtgaa cgaaatccct gaaactgaga ctgtagaaaa
     2641 taagcttgtc cagactattg gatccaagag atttctacac gattgagcac tgtctcctgc
     2701 aggctcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt tttcgttttg
     2761 gtcctaatag ataaaggata ggtctggtag tgttgttcgt tctcgcaggt aaatcaataa
     2821 tactctgcag ttccgtagac ttttcagtgg gacagggtag cgataacaga tagattgtaa
     2881 taagacacag taggtgctcg tagttgcgtg aagagaaccg ctcaggaaat ccagtcagaa
     2941 gtattggtaa tcgttgaaaa ctcagtcgac gcacttactg aagacgtcct attacactcg
     3001 tcgttggaaa ctgaagatgc ggccgcggaa acacagaaaa aagcccgcac ctgacagtgc
     3061 gggctttttt tttcgaccaa aggtagcgaa cgacgagtca ctgttgagga taaatacttt
     3121 ctctactagc tagctgttac acaggtcctc agcggcgcgc ccgcccggtc atgacgctga
     3181 gttagtggcg cagctgcgct ctgagaacga gtttcgaccc tgacagccgg cgagctgttc
     3241 aggcgaagat cgcacagctg cgcgaggaac tagccgcaat gcccgaacac gccccagcca
     3301 tcccttggag caggtggcag cgtcagggga gtcgggggat gtttggcagg ggatgtggaa
     3361 agagagttcg ctttgctcac atggctcaac cgggtaacta actgatatgg gctcttcgtc
     3421 gcccactttg aacacgccga ggaatggacc acgctgaacg tgactcgcat gcttcactgc
     3481 atgtatggat tcgttcgaga ctttgttccc tgagagctgg ctgccacgca agccgctggc
     3541 gtcagccgag aagtctgggg cgtaccggca cgtgactcgg cagagggcgc tggagctgcc
     3601 ttacatcgaa gcgaacccgt tggtcatgca gtccttggtc atcaccgatc gagatgcttc
     3661 ggatgctgac tgggccgcag acctcgctgg gctgccttca ccgtcctacg tgtccatgaa
     3721 ccgtgtcacg accaccggac acatcgtcta tgccttgaag aaccctgtgt gtctgaccga
     3781 tgccgcgcgg cgacggccta tcaacctgct cgcccgcgtc gagcagggcc tatgcgacgt
     3841 tctcggcggc gatgcatcct acgggcaccg gatcacaaag aacccgctca gcaccgccca
     3901 tgcgaccctc tggggccccg cagacgcgct ctacgagctg cgcgccctcg cacacaccct
     3961 cgacgagatc cacgcactgc cggaggcagg gaacccgcgt cgcaacgtca cccgatcaac
     4021 ggtcggccgc aacgtcaccc tgttcgacac cacccgcatg tgggcatacc gggccgtccg
     4081 gcactcctgg ggcggcccgg tcgccgaatg ggagcacacc gtattcgagc acatccacct
     4141 actgaacgaa acgatcatcg ccgacgagtt cgccacaggc cccctcggct tgaacgaact
     4201 taagcactta tctcgatcca tttcccgatg ggtctggcgc aacttcaccc ccgaaacctt
     4261 ccgcgcacgc cagaaagcga tcagcctccg tggagcatcc aaaggcggca aagaaggcgg
     4321 ccacaaaggc ggcattgcca gtggcgcatc acggcgtgcc catacccgtc aacagttctt
     4381 ggagggtctg tcatgaccac acgtgaacgc ctcccccgca acggctacag catcgccgcc
     4441 gctgcgaaaa agctcggtgt ctccgagtcc accgtcaagc ggtggacttc cgagccacgc
     4501 gaggagttcg tggcccgcgt tgccgcacgc cacgcgcgga ttcgtgagct gcgctcggag
     4561 ggtcagagca tgcgtgcgat tgctgccgag gtcggggttt ccgtgggcac cgtgcactac
     4621 gcgctgaaca agaatcgaac tgacgcatga ccgtaacgcc gcacgatgag cattttcttg
     4681 atcgtgcacc gcttggcact acgttcgcgt gcggttgcac agtgcgcgcc acgttcttat
     4741 cctgcggcca ttgtggctac agccaatggg gggcatcagc aacggacgtt gaacccggtg
     4801 ggcaagtgtt actcaggggg acatgcccag tctgcggcgc tcggattgac ggtatggcag
     4861 tcgtgcatgc ggccccaccg tcaaactcat tcaggtatca gtgagaaccc tcatggcacc
     4921 ccctcgtgac acgttctcgt tgcgatcagc tgcggccaag ctgatcttcg ggcagcgttg
     4981 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
     5041 ggggttgcct tactgatcag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5461 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5521 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5581 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5701 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaggcgcctc accaatgctt
     5761 gatgagggag gcaccgatct cggcgatctg acggttgcgc tcatccatcg tggcctggga
     5821 gccggtcgtg tagatcacca cgatgcggct gggcttaccg tcgggaccga gagccgcgat
     5881 gatgccacgc gagccgcgct caccagcacc cgacttgtcc gcgatgaacc aaccggccgg
     5941 gagcgcgctg cgcaacagcg ggccagcgac cttgtcggcc tccatccagt cgatcagttg
     6001 ctgacgggag gccagagtga ggagttcacc ggtcaggagt ttacggaggg tggtagcca
//
