LOCUS       sequence-451377-        7027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cutibacterium acnes replicative plasmid for recombinant expression. 
            pBRESP36A-based vector optimized for reduced size and modular 
            assembly, harbouring a low-copy pBR322 E. coli ori .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-320_Cacnes_ori
FEATURES             Location/Qualifiers
     source          1..7027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3318..3509
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3503..3525)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3611..3751
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3666..3685
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3766..3783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3937..4525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4017..4036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4608..5399,5400..5468))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5469..5573)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5640..5658
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctagaggaa atcagagctt gaggctgccg gagcgacggt agacccgtcc ggttgggtgg
       61 tgccactgcg tgcactcaag gtcgtttttg gggtgtcaga tgtgacctcg aatgcggaat
      121 tcataggata gattctggaa actttaccgt ccgagctcag gcttacctta ctcgagcaat
      181 aaacagttga tagggcttct ccgttaccat ggttcagcca aaaaacttaa gaccgccggt
      241 cttgtccact accttgcagt aatgcggtgg acaggatcgg cggttttctt ttctcttctc
      301 aattcttctg acctgtaacg aataatagat agtaaagtag tctccgattg agttttctct
      361 gccgagtccc acccagttct gtgatttcag taagttggta attgatacac tgttgcgaga
      421 actggtgcct ggtagtagat aggttgttat tgagtaagaa ggtaaagtga acgaaatccc
      481 tgaaactgag actgtagaaa ataagcttgt ccagactatt ggatccaaga gatttctaca
      541 cgattgagca ctgtctcctg caggctcggt accaaattcc agaaaagagg cctcccgaaa
      601 ggggggcctt ttttcgtttt ggtcctaata gataaaggat aggtctggta gtgttgttcg
      661 ttctcgcagg taaatcaata atactctgca gttccgtaga cttttcagtg ggacagggta
      721 gcgataacag atagattgta ataagacaca gtaggtgctc gtagttgcgt gaagagaacc
      781 gctcaggaaa tccagtcaga agtattggta atcgttgaaa actcagtcga cgcacttact
      841 gaagacgtcc tattacactc gtcgttggaa actgaagatg cggccgcgga aacacagaaa
      901 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggtagcga acgacgagtc
      961 actgttgagg ataaatactt tctctactag ctagctgtta cacaggtcct cagcggcgcg
     1021 cccgcccggt catgacgctg agttagtggc gcagctgcgc tctgagaacg agtttcgacc
     1081 ctgacagccg gcgagctgtt caggcgaaga tcgcacagct gcgcgaggaa ctagccgcaa
     1141 tgcccgaaca cgccccagcc atcccttgga gcaggtggca gcgtcagggg agtcggggga
     1201 tgtttggcag gggatgtgga aagagagttc gctttgctca catggctcaa ccgggtaact
     1261 aactgatatg ggctcttcgt cgcccacttt gaacacgccg aggaatggac cacgctgaac
     1321 gtgactcgca tgcttcactg catgtatgga ttcgttcgag actttgttcc ctgagagctg
     1381 gctgccacgc aagccgctgg cgtcagccga gaagtctggg gcgtaccggc acgtgactcg
     1441 gcagagggcg ctggagctgc cttacatcga agcgaacccg ttggtcatgc agtccttggt
     1501 catcaccgat cgagatgctt cggatgctga ctgggccgca gacctcgctg ggctgccttc
     1561 accgtcctac gtgtccatga accgtgtcac gaccaccgga cacatcgtct atgccttgaa
     1621 gaaccctgtg tgtctgaccg atgccgcgcg gcgacggcct atcaacctgc tcgcccgcgt
     1681 cgagcagggc ctatgcgacg ttctcggcgg cgatgcatcc tacgggcacc ggatcacaaa
     1741 gaacccgctc agcaccgccc atgcgaccct ctggggcccc gcagacgcgc tctacgagct
     1801 gcgcgccctc gcacacaccc tcgacgagat ccacgcactg ccggaggcag ggaacccgcg
     1861 tcgcaacgtc acccgatcaa cggtcggccg caacgtcacc ctgttcgaca ccacccgcat
     1921 gtgggcatac cgggccgtcc ggcactcctg gggcggcccg gtcgccgaat gggagcacac
     1981 cgtattcgag cacatccacc tactgaacga aacgatcatc gccgacgagt tcgccacagg
     2041 ccccctcggc ttgaacgaac ttaagcactt atctcgatcc atttcccgat gggtctggcg
     2101 caacttcacc cccgaaacct tccgcgcacg ccagaaagcg atcagcctcc gtggagcatc
     2161 caaaggcggc aaagaaggcg gccacaaagg cggcattgcc agtggcgcat cacggcgtgc
     2221 ccatacccgt caacagttct tggagggtct gtcatgacca cacgtgaacg cctcccccgc
     2281 aacggctaca gcatcgccgc cgctgcgaaa aagctcggtg tctccgagtc caccgtcaag
     2341 cggtggactt ccgagccacg cgaggagttc gtggcccgcg ttgccgcacg ccacgcgcgg
     2401 attcgtgagc tgcgctcgga gggtcagagc atgcgtgcga ttgctgccga ggtcggggtt
     2461 tccgtgggca ccgtgcacta cgcgctgaac aagaatcgaa ctgacgcatg accgtaacgc
     2521 cgcacgatga gcattttctt gatcgtgcac cgcttggcac tacgttcgcg tgcggttgca
     2581 cagtgcgcgc cacgttctta tcctgcggcc attgtggcta cagccaatgg ggggcatcag
     2641 caacggacgt tgaacccggt gggcaagtgt tactcagggg gacatgccca gtctgcggcg
     2701 ctcggattga cggtatggca gtcgtgcatg cggccccacc gtcaaactca ttcaggtatc
     2761 agtgagaacc ctcatggcac cccctcgtga cacgttctcg ttgcgatcag ctgcggccaa
     2821 gctgatcttc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     2881 tgaggacccg gctaggctgg cggggttgcc ttactgatca gcagaatgaa tcaccgatac
     2941 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     3001 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     3061 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     3121 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     3181 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     3241 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     3301 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     3361 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     3421 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     3481 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     3541 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     3601 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     3661 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     3721 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     3781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4561 tcagtggaac gaaaactcac gttaagggat tttggtcatg aggcgcctca ccaatgcttg
     4621 atgagggagg caccgatctc ggcgatctga cggttgcgct catccatcgt ggcctgggag
     4681 ccggtcgtgt agatcaccac gatgcggctg ggcttaccgt cgggaccgag agccgcgatg
     4741 atgccacgcg agccgcgctc accagcaccc gacttgtccg cgatgaacca accggccggg
     4801 agcgcgctgc gcaacagcgg gccagcgacc ttgtcggcct ccatccagtc gatcagttgc
     4861 tgacgggagg ccagagtgag gagttcaccg gtcaggagtt tacggagggt ggtagccata
     4921 gccgcgggca tagtcgtgtc gcgctcgtcg ttcgggatag cctcattaag ttcgggttcc
     4981 caacggtcca gacgggtcac gtggtcgccc atgttgtgga gaaaagccgt cagctctttc
     5041 gggccaccga tggtcgtcaa cagcagattg gcggcagtgt tgtcggacat agtgatggcc
     5101 gcggagcaca gctcgcgcac ggtcatccca tcggtcagat gtttctccgt gaccggggag
     5161 tattcgacca ggtcgttctg ggagtaatga atgcgacggc ccagttgctc ctgcccagca
     5221 tccacccggc taagcacagc gccgcagagg aggaccttga acgtggacat catggggaaa
     5281 cgctcctcag ggcggaaaga ctccagaatc ttaccggagt tcaggtccaa ctcgatataa
     5341 ccgacgcgcg cccccagctg atcctcggcg tccttgacct tgaccagggt ttccggatgg
     5401 gcaaaaaccg gcaagcaaaa ggccgcaaaa aatgggataa gggcaacgcg gaagtgctgg
     5461 atcgacatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgactagtt
     5581 ccccgaaaag tgccacctga cgttaagaaa ccattattat catgacatta acctataaaa
     5641 ataggcgtat cacgaggccc tttcgtctac aatcagcgct gacccggacc gccacgacgg
     5701 cctggacgac cgccggtacg accaccaccc gaagggccac gacgaccacc ctggtcgccc
     5761 tgctcgcgct ccccggtacg accatcgtcg cgattacggc cagaggaccg acggtcaccg
     5821 tggccggagg cctgacggtc gcgacggtcc tgaccacccg tccgacggtc gccacgatcc
     5881 tggccacccg tacggcggtc accacgatcc tgaccacccg tacgacggcc gcgctgcggt
     5941 tggccaccgc gcggctcgtt cttctggtca aggcgctcga agagccgcag ccattgctcc
     6001 ggccggacgt aggccacgac agcaccacca cccacgccgg catggtccag cgcagcctcc
     6061 acgcgacgac gagggtactt gcgcaggagg gaggcttgaa tgttgccgcc gacaccagta
     6121 aaacacaact ccaccatgct ctcgtagcgc tcaagggcag caccctccaa cagcggttca
     6181 gcgcggcgac gcagacgcat gatggcggaa tcgaccttcg ggaccggctt gaacaagcgg
     6241 cgatcgacct tctcgacgaa ctcccactcg aagagcggcc aagtcatgac cgtgaggcgg
     6301 gaccagcggc cataatcacc ggtgcgctta cgggcaaatt cgagttgggt aaccatcgtg
     6361 gcggtttcga tcgtcggagc ctccaggcac caatcgacga tcgcagacgt gatgccgtac
     6421 gggatcgcgc cgacgaaggc aaacggctcg ggagggggct cggcggtcag gaagtccgcg
     6481 ttgacgacct cgatattcgg gtggccggac agcttctcac gcagggactt cgccaagcgc
     6541 gggtcgatct cgtagctcgt cacctggcgg gcccgatcgg caagctcacg ggtcagcaga
     6601 ccctcgcctg ggccagcctc caggaccgga aggtcggggc gcagttcggc cgtttccgcg
     6661 atgcgggcga tcgtcttacg gtcacgaagg aaattctgac cgaattgacg gcggttgcgg
     6721 tcccgctcag tacggcccag gaccgggcgg ttctggttcg ggtgctggcg gtcctggttg
     6781 cggcgacgcg ggcgcggttg ctcgtcggac gacgacatga cgatctcccg atgtgtcgta
     6841 cgtatgggaa ttgacgaaaa tcatatagat tttcagttga agtgtagcac tgcatagtgt
     6901 catcgaagtg tcggcggcaa cggtatgccc ggtagggggt gggtctgcct ttcggtttcg
     6961 tgaacctgat gcggcgacgc gcatgtagcc gataacccct agactcgccc gtgcagatcg
     7021 tcggtgt
//
