LOCUS       sequence-451400-       10847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. acnes with tetR-based inducible expression of mCherry. pBRESP36A 
            with P(PPA_RS09745)+RBS_2+TetR // 
            P(BBa_23119-TetO1O3)+RBS_1+mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10847)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 10847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-304_Cacnes_tetR
FEATURES             Location/Qualifiers
     source          1..10847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             789..800
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             801..842
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             852..869
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5322..5513
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(5507..5529)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5615..5755
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5670..5689
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5770..5787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5941..6529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6021..6040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6700..7491,7492..7560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7323..7342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7561..7665)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7733..7751
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7816..7834
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    9202..9220
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     RBS             9717..9739
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             10368..10385
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    10760..10778
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        10779..10813
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     protein_bind    10814..10832
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 gtagtgctta tcagacccaa tactgttgaa cagcctgcgg tccggtccac ttataaatat
       61 agaggaggtt tgatatggtg tccaaaggcg aggaggataa catggcgatc atcaaggagt
      121 tcatgcgctt caaggtccac atggagggct ccgtcaacgg ccacgaattt gagatcgaag
      181 gtgaggggga gggccggccg tacgaaggca cgcagacggc caagctgaag gtcaccaaag
      241 ggggcccgct gcccttcgcg tgggacatcc tgtcccctca gttcatgtac ggttccaagg
      301 cctacgtcaa gcaccccgcg gacattccgg actacctgaa actctccttc ccggaaggct
      361 tcaaatggga gcgggtcatg aacttcgaag acggcggtgt ggtcaccgtc acccaggact
      421 cgtcccttca agacggtgag tttatctaca aggtcaagct gcgcggtacc aactttccgt
      481 ccgacggccc tgtcatgcag aagaagacta tgggctggga ggccagctcc gagcgtatgt
      541 atccagagga tggcgctctt aagggtgaga tcaagcagag actgaagctc aaggacggcg
      601 gtcactacga cgccgaggtc aagaccacct acaaggcgaa gaaaccggtc cagctgccgg
      661 gcgcttacaa cgtcaacatc aagctggaca tcacttctca caacgaggac tacactatcg
      721 tcgagcaata tgagcgtgcc gagggccgcc actccactgg cggcatggac gagctgtaca
      781 agtcccgcat cgagggccgc ggtaagccga tcccgaaccc gctgttgggc ctggattcca
      841 ctcgcacggg ccaccatcac caccaccact gatcgctggg acgcccggtt tctacacgat
      901 tgagcactgt ctcctgcagg ctcggtacca aattccagaa aagaggcctc ccgaaagggg
      961 ggcctttttt cgttttggtc ctaatagata aaggataggt ctggtagtgt tgttcgttct
     1021 cgcaggtaaa tcaataatac tctgcagttc cgtagacttt tcagtgggac agggtagcga
     1081 taacagatag attgtaataa gacacagtag gtgctcgtag ttgcgtgaag agaaccgctc
     1141 aggaaatcca gtcagaagta ttggtaatcg ttgaaaactc agtcgacgca cttactgaag
     1201 acgtcctatt acactcgtcg ttggaaactg aagatgcggc cgcggaaaca cagaaaaaag
     1261 cccgcacctg acagtgcggg cttttttttt cgaccaaagg tagcgaacga cgagtcactg
     1321 ttgaggataa atactttctc tactaggcgc ctgttacaca ggtcctcagc ggcgcgccga
     1381 aactgaacct gttattttac gctacggacg cccggtcatg acgctgagtt agtggcgcag
     1441 ctgcgctctg agaacgagtt tttacggcgt caggtcgagc agcaggcgcg cacgatcgaa
     1501 cggcaggctg aggcacacgc ggtggtctca gcgcagctca cacgggttgg ccagcttgag
     1561 gccggcgacg cagcagcacc gacactggca cccgttgaaa ggccggctcc gcgacggcgg
     1621 tggtggcagc gtcggtagcg gtcaggatcg ctctggcgtg acgagtgtgt ctggcagtgc
     1681 gaacagttgc tcgaccagtg gcagcagaag cgagatcgct gcgtggtgct gttcctcggt
     1741 cagttcgtcg aggactggcg ggtcttgctg cgtccagccg atcgcctcgg cggccaaggt
     1801 cagttccaag ctgtgccaac gcacacgccc ctcggctgac agctgagtct cgaactgtgc
     1861 aactggaccg gccggaagat gcacgttgcc gaggtcgtga gtggccaagc gcacgtcaaa
     1921 gagtgctgct tcgtagccgc gcagaaatgg cagtgctcgg tcgattcgga tcggcctgcc
     1981 caggtacatt ccgggccgct tgatgaacgc ctccgcgtag aagcgcaccg ttctcggccc
     2041 ggcctcgtga tctgtcactg tgcacgctcc tctcgatggt tctcgacgct accggagacc
     2101 accgacgttc atgcccagcg cagcgacctg aaaggaccaa gccgagttag ccgtgctaac
     2161 cgtatagctt gctccgtcgc ctctgagggc aaccacctgc gcagcaggtg ggcggcagcc
     2221 cgcgcgcaag cgcctaccgg gtttgggcac agcccataaa tcaacgcctc cggtgttgaa
     2281 gcgatcgtgt gtcacgattg ctatgcttgc taccccttca gggttttcgt atacacaaat
     2341 caagtttttt cgtatacgct aatgccatga gtgagcatct actgcacggc aagcccgtca
     2401 ccaacgagca gattcaggca tgggcagacg aggccgaggc cggatacgac ctgcccaaac
     2461 tccccaagcc acggcgcgga cgcccgcccg taggagacgg tccgggcacc gtcgtacccg
     2521 tgcgtctcga cgcggccacc gttgccgctc tcacagaacg agcaacagcc gagggcatca
     2581 cgaaccgttc agacgcgatc cgagccgcag tccacgagtg gacacgggtt gcctgacctc
     2641 cacgactcag cacgcaagca ctaccaacga gaccggctcg acgacacggc cgtgctctac
     2701 gcggccaccc acgttctcaa ctcccggcca ctcgacgacg aagacgaccc gcgccgctgg
     2761 ctcatgatcg gaaccgaccc agcaggccgc ctactcgaac tcgtcgcact gatctacgac
     2821 gacggctacg aactgatcat ccacgcaatg aaagcccgca cccaatacct cgaccagctc
     2881 taaccaagaa aggaacctga tgagcgacca gctagacagc gaccgcaact acgacccgat
     2941 gatcttcgac gtgatgcgcg agaccgcgaa ccgcgtcgtc gccacgtacg ttgcatggga
     3001 agatgaagcc gctgatcccc gcgaggctgc gcactggcag gccgagcgat tccgcacccg
     3061 gcacgaggtg cgcgccgtcg accctgacag ccggcgagca gttcaggcga agatcgcaca
     3121 gctgcgcgag gaactagccg caatgcccga acacgcccca gccatccctt ggagcaggtg
     3181 gcagcgtcag gggagtcggg ggatgtttgg caggggatgt ggaaagagag ttcgctttgc
     3241 tcacatggct caaccgggta actaactgat atggggtctt cgtcgcccac tttgaacacg
     3301 ccgaggaatg gaccacgctg aacgtgactc gcatgcttca ctgcatgtat ggattcgttc
     3361 gagacgttgt tccctgagag ctggctgcca cgcaagccgc tggcgtcagc cgagaagtct
     3421 ggggcgtacc ggcacgtgac tcggcagagg gcgctggagc tgccttacat cgaagcgaac
     3481 ccgttggtca tgcagtcctt ggtcatcacc gatcgagatg cttcggatgc tgactgggcc
     3541 gcagacctcg ctgggctgcc ttcaccgtcc tacgtgtcca tgaaccgtgt cacgaccacc
     3601 ggacacatcg tctatgcctt gaagaaccct gtgtgtctga ccgatgccgc gcggcgacgg
     3661 cctatcaacc tgctcgcccg cgtcgagcag ggcctatgcg acgttctcgg cggcgatgca
     3721 tcctacgggc accggatcac aaagaacccg ctcagcaccg cccatgcgac cctctggggc
     3781 cccgcagacg cgctctacga gctgcgcgcc ctcgcacaca ccctcgacga gatccacgca
     3841 ctgccggagg cagggaaccc gcgtcgcaac gtcacccgat caacggtcgg ccgcaacgtc
     3901 accctgttcg acaccacccg catgtgggca taccgggccg tccggcactc ctggggcggc
     3961 ccggtcgccg aatgggagca caccgtattc gagcacatcc acctactgaa cgagacgatc
     4021 atcgccgacg aattcgccac aggccccctc ggcttgaacg aacttaagca cttatctcga
     4081 tccatttccc gatgggtctg gcgcaacttc acccccgaaa ccttccgcgc acgccagaaa
     4141 gcgatcagcc tccgtggagc atccaaaggc ggcaaagaag gcggccacaa aggcggcatt
     4201 gccagtggcg catcacggcg cgcccatacc cgtcaacagt tcttggaggg tctctcatga
     4261 ccacacgtga acgtctcccc cgcaacggct acagcatcgc cgctgctgcg aaaaagctcg
     4321 gtgtctccga gtccaccgtc aagcggtgga cttccgagcc acgcgaggag ttcgtggccc
     4381 gcgttgccgc acgccacgcg cggattcgtg agctgcgctc ggagggtcag agcatgcgtg
     4441 cgattgctgc cgaggtcggg gtttccgtgg gcaccgtgca ctacgcgctg aacaagaatc
     4501 gaactgacgc atgaccgtaa cgccgcacga tgagcatttt cttgatcgtg caccgcttgg
     4561 cactacgttc gcgtgcggtt gcacagtgcg cgccacgttc ttatcctgcg gccattgtgg
     4621 ctacagccaa tggggggcat cagcaacgga cgttgaaccc ggtgggcaag tgttactcag
     4681 ggggacatgc ccagtctgcg gcgctcggat tgacggtatg gcagtcgtgc atgcggcccc
     4741 accgtcaaac tcattcaggt atcagtgaga accctcatgg caccccctcg tgacacgttc
     4801 tcgttgcgat cagctgcggc caagcttgat cttcgggcag cgttgggtcc tggccacggg
     4861 tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg
     4921 gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt
     4981 ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa
     5041 cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc
     5101 taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt
     5161 ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc
     5221 gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca
     5281 ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa
     5341 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca
     5401 acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg
     5461 agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     5521 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     5581 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     5641 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     5701 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     5761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6361 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     7021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     7321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7681 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     7741 tatcacgagg ccctttcgtc ttcaagaatt caagcttgtc gacgttaacc tgcaggcatg
     7801 cggatccggt accagctgtc tcttatacac atctctgcag ttaacgctga cccggaccac
     7861 cacgacgacc cggacgacca ccggtacgac caccaccaga cggaccgcgg cgaccaccct
     7921 ggtcaccctg ttcacgttca ccggtacgac cgtcgtcacg gttacgacca gaagaacgac
     7981 ggtcaccgtg accagacgct tgccggtctc tgcgatcttg gcccccggta cgtctgtcac
     8041 cacggtcctg accaccggta cgacggtcgc ccctatcctg tccacccgtc ctacgaccac
     8101 gctgcggctg accaccacgc ggttcgtttt tctggtccag acgttcgaac agacgcagcc
     8161 actgttccgg acgaacgtaa gcaacaacag caccaccacc aacaccagcg tggtccagag
     8221 cagcttcaac acgacgacgc gggtatttac gcagcagaga agcctggatg ttaccaccaa
     8281 caccggtgaa gcacagttca accatagatt cgtaacgttc cagagcagca ccttccagca
     8341 gcggttcagc acgacgacgc agacgcatga tagcagagtc aactttcgga accggtttga
     8401 acagacgacg atcgactttt tcaacgaatt cccattcgaa cagcggccag gtcataacgg
     8461 tcagacgaga ccaacgaccg tagtcaccgg tacgtttacg agcgaattcc agctgggtaa
     8521 ccatggtagc ggtttcgatg gtcggagctt ccaggcacca gtcaacgata gcagaggtga
     8581 taccgtacgg gatagcacca acgaaagcga acggttccgg cggcggttca gcggtcagga
     8641 agtcagcgtt aacaacttcg atgttcgggt gaccagacag tttttcacgc agagatttag
     8701 ccagacgcgg gtcgatttcg taagaggtaa cctgacgagc acggtcagcc agttcacggg
     8761 tcagcagacc ttcacccgga ccagcttcca gaaccggcag gtccggacgc agttcagcgg
     8821 tttcagcgat acgagcgatg gttttacggt cacgcaggaa gttctggccg aattggcggc
     8881 ggttacggtc acgctcggta cggccgagga cggggcgatt ctggttgggg tgctggcggt
     8941 cttggttgcg gcggcggggg cggggctgtt catcggagga ggacatgacg atctcccgat
     9001 gtgtcgtacg tatgggaatt gacgaaaatc atatagattt tcagttgaag tgtagcactg
     9061 catagtgtca tcgaagtgtc ggcggcaacg gtatgcccgg tagggggtgg gtctgccttt
     9121 cggtttcgtg aacctgatgc ggcgacgcgc atgtagccga taacccctag actcgcccgt
     9181 gcagatcgtc ggtgtggatc cagatgtgta taagagacag ctggtaccgg ccgctgctgt
     9241 gcgtgcgggc gtgagcgttt ctacgctgcg gcgcaggaaa tcagagcttg aggctgccgg
     9301 agcgacggta gacccgtccg gttgggtggt gccactgcgt gcactcaagg tcgtttttgg
     9361 ggtgtcagat gagacctcga atgcgaatac cagtaaatcc tgacctgtga gttgtgaatt
     9421 cataggatag attctggaaa ctttaccgtc cgagctccag cctgcggtcc ggacaccgac
     9481 gatctgcacg ggcgagtcta ggggttatcg gctacatgcg cgtcgccgca tcaggttcac
     9541 gaaaccgaaa ggcagaccca ccccctaccg ggcataccgt tgccgccgac acttcgatga
     9601 cactatgcag tgctacactt caactgaaaa tctatatgat tttcgtcaat tctcgctggg
     9661 acgcccgtag aactctgtga ggataaagtc tcccttacag cctgcggtcc ggtaattttg
     9721 tttaacttta agaaggagat atacatatgt caagactaga taagagcaag gtcattaaca
     9781 gcgcactgga gttactaaac gaagttggaa tagagggatt gactacacga aaattagcac
     9841 agaaattagg agtagaacaa ccgacacttt attggcacgt taaaaataaa agagctttgc
     9901 tggatgcttt agctatagaa atgctagacc gacaccacac acatttttgc cccttagaag
     9961 gagaaagttg gcaagatttc ctacgtaaca acgccaaatc atttagatgc gcgctattat
    10021 cacaccgcga cggcgccaaa gttcacctcg ggacaagacc tacggagaag caatacgaaa
    10081 ccctagagaa tcaattagcc tttctatgtc aacaaggatt cagtttggaa aacgccctct
    10141 acgccctatc agccgttggt cattttacat tgggatgcgt tttagaggat caggaacatc
    10201 aagtagcaaa agaagaaaga gagacaccca ccaccgacag catgccacca ttattacgtc
    10261 aagctattga actattcgat catcagggag cggaaccggc atttttattt ggcctcgaat
    10321 taatcatatg tggtttagaa aaacaattaa agtgtgaatc tggatcccat catcaccatc
    10381 accactgata atcgctggga cgcccgctcg agcaataaac agttgatagg gcttctccgt
    10441 taccatggtt cagccaaaaa acttaagacc gccggtcttg tccactacct tgcagtaatg
    10501 cggtggacag gatcggcggt tttcttttct cttctcaatt cttctgacct gtaacgaata
    10561 atagatagta aagtagtctc cgattgagtt ttctctgccg agtcccaccc agttctgtga
    10621 tttcagtaag ttggtaattg atacactgtt gcgagaactg gtgcctggta gtagataggt
    10681 tgttattgag taagaaggta aagtgaacga aatccctgaa actgagactg tagaaaataa
    10741 gcttcagcct gcggtccggt ccctatcagt gatagagatt gacagctagc tcagtcctag
    10801 gtataatgct agctccctat cagtgataga gatcgctggg acgcccg
//
