LOCUS       sequence-451402-        5499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Suicide C. acnes vector derivative of pEND-350 optimized for 
            recombination into the CAMP2 locus, with BxbI recombination sites 
            flanking EryR resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-400_Cacnes_CAMP2
FEATURES             Location/Qualifiers
     source          1..5499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    188..236
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     protein_bind    1714..1771
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attP site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(2398..2986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2478..2497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3069..3860,3861..3929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3930..4034)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agatgcggcc gcggaaacac agaaaaaagc ccgcacctga cagtgcgggc tttttttttc
       61 gaccaaaggt agcgaacgac gagtcactgt tgaggataaa tactttctct actagctagc
      121 tgttacacag gtcctcagcg gcgcgccata gcgccaacca tgcaccatag ccagcctgac
      181 agtagcccgg ccggcttgtc gacgacggcg gtctccgtcg tcaggatcat ccgggcgcag
      241 ctgagagcga cacagtcctg tacttgggat gctggcacac cgacgatctg cacgggcgag
      301 tctaggggtt atcggctaca tgcgcgtcgc cgcatcaggt tcacgaaacc gaaaggcaga
      361 cccaccccct accgggcata ccgttgccgc cgacacttcg atgacactat gcagtgctac
      421 acttcaactg aaaatctata tgattttcgt caattcccat acgtacgaca catcgggaga
      481 tcgtcatgtc gtcgtccgac gagcaaccgc gcccgcgtcg ccgcaaccag gaccgccagc
      541 acccgaacca gaaccgcccg gtcctgggcc gtactgagcg ggaccgcaac cgccgtcaat
      601 tcggtcagaa tttccttcgt gaccgtaaga cgatcgcccg catcgcggaa acggccgaac
      661 tgcgccccga ccttccggtc ctggaggctg gcccaggcga gggtctgctg acccgtgagc
      721 ttgccgatcg ggcccgccag gtgacgagct acgagatcga cccgcgcttg gcgaagtccc
      781 tgcgtgagaa gctgtccggc cacccgaata tcgaggtcgt caacgcggac ttcctgaccg
      841 ccgagccccc tcccgagccg tttgccttcg tcggcgcgat cccgtacggc atcacgtctg
      901 cgatcgtcga ttggtgcctg gaggctccga cgatcgaaac cgccacgatg gttacccaac
      961 tcgaatttgc ccgtaagcgc accggtgatt atggccgctg gtcccgcctc acggtcatga
     1021 cttggccgct cttcgagtgg gagttcgtcg agaaggtcga tcgccgcttg ttcaagccgg
     1081 tcccgaaggt cgattccgcc atcatgcgtc tgcgtcgccg cgctgaaccg ctgttggagg
     1141 gtgctgccct tgagcgctac gagagcatgg tggagttgtg ttttactggt gtcggcggca
     1201 acattcaagc ctccctcctg cgcaagtacc ctcgtcgtcg cgtggaggct gcgctggacc
     1261 atgccggcgt gggtggtggt gctgtcgtgg cctacgtccg gccggagcaa tggctgcggc
     1321 tcttcgagcg ccttgaccag aagaacgagc cgcgcggtgg ccaaccgcag cgcggccgtc
     1381 gtacgggtgg tcaggatcgt ggtgaccgcc gtacgggtgg ccaggatcgt ggcgaccgtc
     1441 ggacgggtgg tcaggaccgt cgcgaccgtc aggcctccgg ccacggtgac cgtcggtcct
     1501 ctggccgtaa tcgcgacgat ggtcgtaccg gggagcgcga gcagggcgac cagggtggtc
     1561 gtcgtggccc ttcgggtggt ggtcgtaccg gcggtcgtcc aggccgtcgt ggcggtccgg
     1621 gtcagcgctg atctagaccc gcacttaacc cgcttcggcg ggtttttgtt ttttggggtg
     1681 caggtaggcc atattgtctt cggggcccca ctcgtcgtgg tttgtctggt caaccaccgc
     1741 ggtctcagtg gtgtacggta caaaccccga cgaggcgata agctcggcaa ggctgattac
     1801 gcctgctcgt gcagcctgcg gtccgggctg gtaccgtcag aacgcactcg ctaattgaca
     1861 tcaacactcg gtgccttgcc cgcctgcgtg cgggcaaggc accgagtgcg ctgtgcagaa
     1921 gaaaattgtc tttacgcgag gcgccgtaac acacatcttc tctccgaggc ggtgcgcacc
     1981 actatcgcca ccctcatccc tcgggaaaac ttaccgagcg tttccggtag cgtcgccgca
     2041 cagcttcggc aatgtccatt cattaggggg cgacaaccgt gacgatgcac cttgataccc
     2101 ggggtcacaa cgacgttcaa caccatgtcg agatccacgt gtacgtagcc gtcgtccaga
     2161 cagttcatcg tctcacgcga gatcgcggca tgatcagcta ccaccgccgt aagcaggcca
     2221 gcgccttgta ccaggcgcca ggcgcgcgtt aatcgacgta attcacctgc acagcggagt
     2281 atacggatcc gccaaccatg agtcatcatg tcaaggacgt atgtacgcgg catcgtcgct
     2341 gggacgcccg ctaggctggc ggggttgcct tactgatcag gccgcgttgc tggcgttttt
     2401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2821 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2941 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3001 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3061 gaggcgcctc accaatgctt gatgagggag gcaccgatct cggcgatctg acggttgcgc
     3121 tcatccatcg tggcctggga gccggtcgtg tagatcacca cgatgcggct gggcttaccg
     3181 tcgggaccga gagccgcgat gatgccacgc gagccgcgct caccagcacc cgacttgtcc
     3241 gcgatgaacc aaccggccgg gagcgcgctg cgcaacagcg ggccagcgac cttgtcggcc
     3301 tccatccagt cgatcagttg ctgacgggag gccagagtga ggagttcacc ggtcaggagt
     3361 ttacggaggg tggtagccat agccgcgggc atagtcgtgt cgcgctcgtc gttcgggata
     3421 gcctcattaa gttcgggttc ccaacggtcc agacgggtca cgtggtcgcc catgttgtgg
     3481 agaaaagccg tcagctcttt cgggccaccg atggtcgtca acagcagatt ggcggcagtg
     3541 ttgtcggaca tagtgatggc cgcggagcac agctcgcgca cggtcatccc atcggtcaga
     3601 tgtttctccg tgaccgggga gtattcgacc aggtcgttct gggagtaatg aatgcgacgg
     3661 cccagttgct cctgcccagc atccacccgg ctaagcacag cgccgcagag gaggaccttg
     3721 aacgtggaca tcatggggaa acgctcctca gggcggaaag actccagaat cttaccggag
     3781 ttcaggtcca actcgatata accgacgcgc gcccccagct gatcctcggc gtccttgacc
     3841 ttgaccaggg tttccggatg ggcaaaaacc ggcaagcaaa aggccgcaaa aaatgggata
     3901 agggcaacgc ggaagtgctg gatcgacata ctcttccttt ttcaatatta ttgaagcatt
     3961 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4021 ataggggttc cgcgactagt tccccgaaaa gtgccacctg acgttaagaa acccattatt
     4081 acagcctgcg gtccggtcag cgatagccgt atgaactgtt tcattgactc aacttgaccg
     4141 attcagtgcc atgcaaacac gtgatgatga agctaacctg gacacgcatc cgacacccga
     4201 tcagggggta ttattcctga agaccaaatg ctccacactg ggatgaactc aaccatatcg
     4261 tgggcacctc agagcagatc ctcagaaagg cactttcacc agttcttgac ctccaagatt
     4321 acagaacgga agatctcgaa ttgcccgcgc attaacttca tattcggtgt attgggcata
     4381 ccatctggtc acgcctgcaa caagcctaga cgggtctatg caccgtcgta gccatacacc
     4441 acacgggccc ctgtcggaat gacgcacgcc tgatcacagc tccaacaaat gttggcggcg
     4501 tacacgccgt tcaccacacc gacccccatc caaaatatcc gatacacttc gcttgcctcg
     4561 accggggcat ccgcaaccaa taaacggaga accttttcgc tgggacgccc gctagatcaa
     4621 tgtgtacgtc gagtgcggta ttcctctggg ccggccggct tgtcgacgac ggcgcactcc
     4681 gtcgtcagga tcatccgggc cgtggtgcgg atctagctac ccgcacttga gtccacggct
     4741 gaattcatag gatagattct ggaaacttta ccgtccgagc tcaggcttac cttactcgag
     4801 caataaacag ttgatagggc ttctccgtta ccatggttca gccaaaaaac ttaagaccgc
     4861 cggtcttgtc cactaccttg cagtaatgcg gtggacagga tcggcggttt tcttttctct
     4921 tctcaattct tctgacctgt aacgaataat agatagtaaa gtagtctccg attgagtttt
     4981 ctctgccgag tcccacccag ttctgtgatt tcagtaagtt ggtaattgat acactgttgc
     5041 gagaactggt gcctggtagt agataggttg ttattgagta agaaggtaaa gtgaacgaaa
     5101 tccctgaaac tgagactgta gaaaataagc ttgtccagac tattggatcc aagagatttc
     5161 tacacgattg agcactgtct cctgcaggct cggtaccaaa ttccagaaaa gaggcctccc
     5221 gaaagggggg ccttttttcg ttttggtcct aatagataaa ggataggtct ggtagtgttg
     5281 ttcgttctcg caggtaaatc aataatactc tgcagttccg tagacttttc agtgggacag
     5341 ggtagcgata acagatagat tgtaataaga cacagtaggt gctcgtagtt gcgtgaagag
     5401 aaccgctcag gaaatccagt cagaagtatt ggtaatcgtt gaaaactcag tcgacgcact
     5461 tactgaagac gtcctattac actcgtcgtt ggaaactga
//
