LOCUS       sequence-451405-       12167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  enSERT LacZ reporter vector for site-specific integration into the 
            H11 locus (contains human beta-globin minimal promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Darbellay F, Ramisch A, Lopez-Delisle L, Kosicki M, Rauseo A, Jouini
            Z, Visel A, Andrey G
  TITLE     Pre-hypertrophic chondrogenic enhancer landscape of limb and axial 
            skeleton development.
  JOURNAL   Nat Commun. 2024 Jun 6;15(1):4820. doi: 10.1038/s41467-024-49203-2.
  PUBMED    38844479
REFERENCE   2  (bases 1 to 12167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-6"; volume: "15(1):4820. doi"; pages: " 
            10.1038/s41467-024-49203-2"
COMMENT     Sequence Label: PCR4-beta-globin::lacZ-H11
FEATURES             Location/Qualifiers
     source          1..12167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..39
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     23..39
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2620..5667
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     polyA_signal    5815..5949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5852..5871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5906..5925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5946..5962)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5946..5962)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8344..8362)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8383..8399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8383..8399)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8396..8418)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8407..8423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8431..8437,8438..8455,8456..8461))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8476..8497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8614..8631)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8785..9373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8865..8884)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(9497..9565,9566..10357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9715..9734)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(10607..11401)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(10607..11401)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10718..10737)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11328..11347
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(11750..12052)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(11852..11871)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
       61 tagggcgaat tgaatttagc cctggtacat gtatggtggc aagtgttcac cacatccacc
      121 tccttgtaag tatccaaatc aacaaaagta gtaaactttg acatttttag gaacatctac
      181 aactcatgag tttcttcttt ctccccatca ggtgtgcttc ctcctgggat agacatggct
      241 cctttacagg ggctatctgg cccactcctg ggtcaaccct tgtacccttt ggtctctgct
      301 gctagtcacc ctctcctaaa ccctcgtcct gggacccctc tgcatttggc ggtgatgcag
      361 cagcagctac agcgctcagg taaggtttag aaataggcca ggttagctga tcacactgga
      421 aagttctgag atttccagga gcaatagtta tcactgcccc tttgtttctt aggtgtcact
      481 agaaaaacag gtgaagtgaa ctgacctgtg cagtctcagg gattattact actgcaagga
      541 cagtcggagc cagccatgct ctagctctgt ttttcacttc ttataagcct gagaattttt
      601 gctgagatgt gaacatgtca gccttggtgg gctttcttac gttgcatttt cttacgttgc
      661 attttgcact ttaggaagcc tttggttaaa tcctctggta gacttcccat cccccaaagt
      721 aacaaaccat tcaagcaaag taggtcagaa aatattgtca atactgagaa caggacttgg
      781 gagtgagtat ccaagggtgg tcttagtaga cttgtggggt tctgtgggtg aggtgtagag
      841 tagagctact ttgctgcact gacactcttc tctccatagt tctgcatcct ccaggctcta
      901 gttcccaggc agcagctatc agcgttcaga ctcctcagaa tgtacccagc cggtcgggca
      961 tgccccacat gcactcccag ctggagcatc gtaccagcca aaggagcagc tcccctgtgg
     1021 gccttgccaa atggtttggc tcagatgtgc tacagcagcc tctgccctcc atgcccacca
     1081 aagtcatcag tgtagatgaa ctggaatata gacagtgaag agggcaggct ggctcaccca
     1141 tacctggacc tgtggtgaca ccctggtcat gactctcatt ccctctttgt aatgggcttt
     1201 tacattggag cacactatgt gaagatgttt aggggatcca catacctacc atgatctaca
     1261 ttatgacaga aggctgttaa atcgaatgaa cctacatggt tcaaatacaa gggatacaag
     1321 attgtcagtc ctggaagtct ttcttttata aaatatgtga atgaagtgtt ggtgtcttct
     1381 agaggtgaca cctaagggtt ctgaaaaaat aaaatgtata gacccttatg tacagacctg
     1441 tgtataaact tttgtacata caaatagggt agcttttttt gaacttatac atacagctgt
     1501 acataaagta actatcagtt aggcttgtgt caactgtttg gatttttttc acttgaatat
     1561 ttgggacttt ttcttttggt ttattaaaag ttacatatgc cacgtgtgtg aacgatatgg
     1621 ctggtactgt gtttatttct tccatgaact aagacagtct aaatgagttc ctttcacgtt
     1681 ttaattttac cttaggactt ctggaatttc ttctgcacat aaagttctga tagcattagt
     1741 ttaagctgga ctaaccctga aagtagcttg tggcaagtat caaggaatca atattatact
     1801 ctacaaaatc aaagtttaca gagaagtcat atagtaattt ttctgaaatt tactggcaca
     1861 atgttaatcc agcctgactc caactaatta atggtcacat taatttaagt ctttcccttg
     1921 cctctgctgc attagtttct ctcaaaattg ttaacttaca acttgaagtc tggtattata
     1981 aattgaatgt aaagcattct gaaagatact atactgattg caggtttttc agtcaggttc
     2041 aagctaattt gaccagtcat tggattaatt atggatctgg ggccataaat gctattttaa
     2101 ttccactata gagattaaaa taagccattc tccatttcat aatattctat tggactttga
     2161 ctgcaggggc ctccaagtct tgacagtaga ttataatcct tcagctgccc actctactgg
     2221 aggaggacaa actggtcact tttcagcaaa acctggctgt ggatcagggc agtctggtac
     2281 ttccaagctc attagatgcc atcatgctct cactgcctcc tcagcttcaa gaggaatctg
     2341 gaaaaagcag tcccactggt caggaaagga acactagtgc acttatcctg ggtgtctgct
     2401 gagccaacag tggtagtaag gtaagggcag gatgtgtcaa actgccaata gagaactact
     2461 tactcttcag gctgaagctg atggaacagc ggccgcagcc cgggctgggc ataaaagtca
     2521 gggcagagcc atctattgct tacatttgct tctagcctgc aggtcgagga gcgcagcctt
     2581 ccagaagcag agcgcggcgc catggctcgc gatgatcccg tcgttttaca acgtcgtgac
     2641 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2701 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2761 ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag
     2821 tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac
     2881 gatgcgccca tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc
     2941 acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag
     3001 gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac
     3061 gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca
     3121 tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt
     3181 tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg
     3241 cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc
     3301 agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg
     3361 gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc
     3421 ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc
     3481 gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg
     3541 cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg
     3601 cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac
     3661 cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat
     3721 atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat
     3781 ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt
     3841 gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg
     3901 atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc
     3961 tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc
     4021 aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc
     4081 accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg
     4141 ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt
     4201 tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg
     4261 tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg
     4321 attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg
     4381 ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca
     4441 gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc
     4501 atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg
     4561 gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa
     4621 ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg
     4681 ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc
     4741 gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac
     4801 gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt
     4861 tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa
     4921 ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta
     4981 agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat
     5041 taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg
     5101 attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc
     5161 taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat
     5221 acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta
     5281 aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt
     5341 gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac
     5401 ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc
     5461 cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg
     5521 ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc
     5581 gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg tcgctaccat
     5641 taccagttgg tctggtgtca aaaataataa taaccgggca ggggggatct aagctctaga
     5701 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
     5761 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     5821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5941 gtctggatcc actagttcta gagcggcaag cagaaaggga acaaggggat attaaatgtg
     6001 tatagacacg ctagagagat ggctcagcag ttaagagaac tagctggtct ttcagaggtc
     6061 ccgagatcaa ttttagacac ccacatggtg gctcatgacc atctatctat aaatggatct
     6121 gattttcatg tctggcagtg tacagaagct aactgaagaa aggtggaaga cccacaagag
     6181 ttcaagataa gccctatata gtgaagttca aggcaagctt tttctacctg aaacttagtc
     6241 tcaaaaaaaa atgaatacgt aaacagtctt ccaggggata agaaccttac agaaaaagca
     6301 gaaatgcctg gggcactgga ttaccgatgt aatcaaattc agtccttgaa ttgaacacag
     6361 gattgcctag agcaaggcca gccagagatt catctcagag ggagaaaggt gtctttggag
     6421 caattttgtg gtaatctagt atgtatcaca taagtttaga cgcatttggg actggaaaga
     6481 tgtgaacaaa gcaccctatg gctcacatct gtcattaact ctagttccag tgcacctgac
     6541 accgtcttct ggcctctgca gtgaccaagc acatgggtag tatgtagaca tatacataag
     6601 caaaacacac atcattaaaa agtgacattt cccaaaggaa gctgaagaac cagttcttga
     6661 gaagatagta gaaatcagaa ggggaaatag tagacataca gagggactga ccaggttgtg
     6721 tcacctttat aggctaggct aattgatgat cgacactagc gctctttgtg aaggacacac
     6781 aaatgagaca tagtttatag gactaaacac acttctaagc aatttaatga gacttaagac
     6841 cctgtctcta gcaaatactc tggatgatat tcagctcaag gctcttgtca gacatgtttc
     6901 cattttcaag gtgagctaac tggccaaaac tgccaacaac ctgtagtgaa tagagaagat
     6961 gagaaaatct ggattctcaa atgacctaat gaaagccact ggagcgccat atggtttctg
     7021 tgaaaatgcc ttttcaatca ttaacctctt aaatgagtgt tagcatccta actaatgagt
     7081 ggtgcagaat agtgggtctg cttagcttaa ctaaggccaa gaaaacaaaa caggaaattc
     7141 attccatgtc atgagactca tactacgagg ttcccttaga cctcaggaga aaaagtcttt
     7201 ggctgtaaga acacacctca gtggatgtgg tagactatgc ctttactctt tttttttttt
     7261 ttttttttgg gttttttcga gacagggttt ctctgtgtag ccttggctgt cctggaactc
     7321 actttgttga ccaggctggc ctcgaactca gagatccgcc tgcctctgcc tctcaagtgc
     7381 tagaattaaa agtgtgcgcc accactccca gctatataga ctatgccttt aatcaggaca
     7441 cttgggaggc agggggatct caaattcaat tccagacaga tgactgacaa acacacacat
     7501 acaccattcg tcttttcttt ttttcttttt tcttttgaaa acaggtttct ctatgtagcc
     7561 ctggctttcc tagaattcaa tctatagacc cagctggcct caaacacaga acctcttgcc
     7621 tctgcctcct caacactatg actaaaggtg tgaaccacca ccaccaccac ccacctggcc
     7681 aaagaatgaa ttgagtgaat aagtacgagt gcaactctcc agcagcctga ggcaagaaag
     7741 cttaacagtt gaacctgaga ccctacttcg tttgtgttgt acatatcttc tcataatgaa
     7801 ctaggcagcc tagtctctct accaaataca ccatgcactt acacctcctc aaaccaactg
     7861 caacagctac acacatttcc atggcttgcc tgtaataata aattcacaaa cttcctctaa
     7921 gtaactttta gtggaaatct accaggttat gcctatttgt gattgggtgc acagatgact
     7981 agatgagatg taatttggga agtgacatgc aataggaaaa atgaatatat aacctataaa
     8041 tagaaaacac caaggcaagc gtggtgactc acacctttaa ttagcacttg ggaggcagaa
     8101 gtcgcatcat ctcttaggtc aaggccaatc tagttccaga ccagcaagga caacagaaac
     8161 cttgccttga tataaacatt ttaaaacttt aaaaatagag aagcccaaac tctgctcatt
     8221 ggtaatctac ccttcctttc tttagacacc ttgagaagct aggctacata gctttgggtc
     8281 cttacgtacc cccactcact cagcctgcag tcaatgaatt cgtttaaacc tgcaggacta
     8341 gtccctttag tgagggttaa ttctgagctt ggcgtaatca tggtcatagc tgtttcctgt
     8401 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8461 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8521 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8701 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8761 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8821 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8881 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8941 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9001 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9061 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9121 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9181 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9241 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9301 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9361 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9421 aaactcacgt taagggattt tggtcatgag acaataaccc tgataaatgc ttcaataata
     9481 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9541 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9601 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9661 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9721 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9781 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9841 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9901 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9961 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
    10021 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
    10081 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
    10141 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
    10201 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    10261 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    10321 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    10381 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10441 ttttgataat ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10501 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat
    10561 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aataattcag aagaactcgt
    10621 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
    10681 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
    10741 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
    10801 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
    10861 cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
    10921 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
    10981 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
    11041 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat
    11101 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
    11161 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt
    11221 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
    11281 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
    11341 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
    11401 tgcgaaacga tcctcatcct gtctcttgat cagagcttga tcccctgcgc catcagatcc
    11461 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
    11521 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
    11581 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
    11641 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
    11701 tgaaaaggat ctaggtgaag atcctttttg ataatctcat gcctgacatt tatattcccc
    11761 agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt
    11821 cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt
    11881 catccccgat atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg
    11941 cactggccag ggggatcacc atccgtcgcc ccggcgtgtc aataatatca ctctgtacat
    12001 ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac tgccgtacgt
    12061 ataggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    12121 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgcca
//
