LOCUS       sequence-451415-        7945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A 
            with P(PPA_RS09745)+RBS_2+PhlF // 
            P(BBa_23119-PhlO1O3)+RBS_1+mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-375_Cacnes_phlF
FEATURES             Location/Qualifiers
     source          1..7945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             418..440
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1048..1065
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        1470..1504
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             2338..2349
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2350..2391
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2401..2418
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      complement(4855..5443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4935..4954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5526..6317,6318..6386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6387..6491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6558..6576
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctagaggaa atcagagctt gaggctgccg gagcgacggt agacccgtcc ggttgggtgg
       61 tgccactgcg tgcactcaag gtcgtttttg gggtgtcaga tgtgacctcg aatgcggaat
      121 tcataggata gattctggaa actttaccgt ccgagctcca gcctgcggtc cggacaccga
      181 cgatctgcac gggcgagtct aggggttatc ggctacatgc gcgtcgccgc atcaggttca
      241 cgaaaccgaa aggcagaccc accccctacc gggcataccg ttgccgccga cacttcgatg
      301 acactatgca gtgctacact tcaactgaaa atctatatga ttttcgtcaa ttctcgctgg
      361 gacgcccgta gaactctgtg aggataaagt ctcccttaca gcctgcggtc cggtaatttt
      421 gtttaacttt aagaaggaga tatacatatg gcgcgcaccc cgtcccgttc ctctatcggc
      481 tccctgcgca gccctcatac gcataaagcg atcctgacga gcaccatcga gatcctgaag
      541 gaatgtgggt acagcggcct gtcgatcgag tccgtggccc gccgtgccgg cgctgggaag
      601 ccgacgatct atcgctggtg gacgaacaag gccgcgctga tcgcggaggt ctacgagaac
      661 gagatcgaac aggtccggaa gttccccgac ctcggctcgt tcaaggccga cctggacttc
      721 cttctgcaca acctgtggaa agtgtggcgc gagactatct gcggcgaggc tttccgctgc
      781 gtgattgccg aggctcagtt ggaccccgtt actctgaccc agttgaaaga tcaattcatg
      841 gagcgccgtc gcgaaatccc caagaaattg gtcgaggacg ccatttctaa cggtgaactc
      901 cctaaggata ttaaccgcga gctgttgctg gacatgattt tcgggttctg ttggtaccgt
      961 ctgcttaccg agcaactcac ggtggagcag gacattgagg aatttacctt tcttttgatt
     1021 aacggcgtct gcccgggcac ccagtgccac caccaccacc atcactgata atcgctggga
     1081 cgcccgctcg agcaataaac agttgatagg gcttctccgt taccatggtt cagccaaaaa
     1141 acttaagacc gccggtcttg tccactacct tgcagtaatg cggtggacag gatcggcggt
     1201 tttcttttct cttctcaatt cttctgacct gtaacgaata atagatagta aagtagtctc
     1261 cgattgagtt ttctctgccg agtcccaccc agttctgtga tttcagtaag ttggtaattg
     1321 atacactgtt gcgagaactg gtgcctggta gtagataggt tgttattgag taagaaggta
     1381 aagtgaacga aatccctgaa actgagactg tagaaaataa gcttcagcct gcggtccgga
     1441 tgatacgaaa cgtaccgtat cgttaaggtt tgacagctag ctcagtccta ggtataatgc
     1501 tagcatgata cgaaacgtac cgtatcgtta aggttcgctg ggacgcccgg tagtgcttat
     1561 cagacccaat actgttgaac agcctgcggt ccggtccact tataaatata gaggaggttt
     1621 gatatggtgt ccaaaggcga ggaggataac atggcgatca tcaaggagtt catgcgcttc
     1681 aaggtccaca tggagggctc cgtcaacggc cacgaatttg agatcgaagg tgagggggag
     1741 ggccggccgt acgaaggcac gcagacggcc aagctgaagg tcaccaaagg gggcccgctg
     1801 cccttcgcgt gggacatcct gtcccctcag ttcatgtacg gttccaaggc ctacgtcaag
     1861 caccccgcgg acattccgga ctacctgaaa ctctccttcc cggaaggctt caaatgggag
     1921 cgggtcatga acttcgaaga cggcggtgtg gtcaccgtca cccaggactc gtcccttcaa
     1981 gacggtgagt ttatctacaa ggtcaagctg cgcggtacca actttccgtc cgacggccct
     2041 gtcatgcaga agaagactat gggctgggag gccagctccg agcgtatgta tccagaggat
     2101 ggcgctctta agggtgagat caagcagaga ctgaagctca aggacggcgg tcactacgac
     2161 gccgaggtca agaccaccta caaggcgaag aaaccggtcc agctgccggg cgcttacaac
     2221 gtcaacatca agctggacat cacttctcac aacgaggact acactatcgt cgagcaatat
     2281 gagcgtgccg agggccgcca ctccactggc ggcatggacg agctgtacaa gtcccgcatc
     2341 gagggccgcg gtaagccgat cccgaacccg ctgttgggcc tggattccac tcgcacgggc
     2401 caccatcacc accaccactg atcgctggga cgcccgggat ccaagagatt tctacacgat
     2461 tgagcactgt ctcctgcagg ctcggtacca aattccagaa aagaggcctc ccgaaagggg
     2521 ggcctttttt cgttttggtc ctaatagata aaggataggt ctggtagtgt tgttcgttct
     2581 cgcaggtaaa tcaataatac tctgcagttc cgtagacttt tcagtgggac agggtagcga
     2641 taacagatag attgtaataa gacacagtag gtgctcgtag ttgcgtgaag agaaccgctc
     2701 aggaaatcca gtcagaagta ttggtaatcg ttgaaaactc agtcgacgca cttactgaag
     2761 acgtcctatt acactcgtcg ttggaaactg aagatgcggc cgcggaaaca cagaaaaaag
     2821 cccgcacctg acagtgcggg cttttttttt cgaccaaagg tagcgaacga cgagtcactg
     2881 ttgaggataa atactttctc tactaggcgc ctgttacaca ggtcctcagc ggcgcgcccg
     2941 cccggtcatg acgctgagtt agtggcgcag ctgcgctctg agaacgagtt tcgaccctga
     3001 cagccggcga gctgttcagg cgaagatcgc acagctgcgc gaggaactag ccgcaatgcc
     3061 cgaacacgcc ccagccatcc cttggagcag gtggcagcgt caggggagtc gggggatgtt
     3121 tggcagggga tgtggaaaga gagttcgctt tgctcacatg gctcaaccgg gtaactaact
     3181 gatatgggct cttcgtcgcc cactttgaac acgccgagga atggaccacg ctgaacgtga
     3241 ctcgcatgct tcactgcatg tatggattcg ttcgagactt tgttccctga gagctggctg
     3301 ccacgcaagc cgctggcgtc agccgagaag tctggggcgt accggcacgt gactcggcag
     3361 agggcgctgg agctgcctta catcgaagcg aacccgttgg tcatgcagtc cttggtcatc
     3421 accgatcgag atgcttcgga tgctgactgg gccgcagacc tcgctgggct gccttcaccg
     3481 tcctacgtgt ccatgaaccg tgtcacgacc accggacaca tcgtctatgc cttgaagaac
     3541 cctgtgtgtc tgaccgatgc cgcgcggcga cggcctatca acctgctcgc ccgcgtcgag
     3601 cagggcctat gcgacgttct cggcggcgat gcatcctacg ggcaccggat cacaaagaac
     3661 ccgctcagca ccgcccatgc gaccctctgg ggccccgcag acgcgctcta cgagctgcgc
     3721 gccctcgcac acaccctcga cgagatccac gcactgccgg aggcagggaa cccgcgtcgc
     3781 aacgtcaccc gatcaacggt cggccgcaac gtcaccctgt tcgacaccac ccgcatgtgg
     3841 gcataccggg ccgtccggca ctcctggggc ggcccggtcg ccgaatggga gcacaccgta
     3901 ttcgagcaca tccacctact gaacgaaacg atcatcgccg acgagttcgc cacaggcccc
     3961 ctcggcttga acgaacttaa gcacttatct cgatccattt cccgatgggt ctggcgcaac
     4021 ttcacccccg aaaccttccg cgcacgccag aaagcgatca gcctccgtgg agcatccaaa
     4081 ggcggcaaag aaggcggcca caaaggcggc attgccagtg gcgcatcacg gcgtgcccat
     4141 acccgtcaac agttcttgga gggtctgtca tgaccacacg tgaacgcctc ccccgcaacg
     4201 gctacagcat cgccgccgct gcgaaaaagc tcggtgtctc cgagtccacc gtcaagcggt
     4261 ggacttccga gccacgcgag gagttcgtgg cccgcgttgc cgcacgccac gcgcggattc
     4321 gtgagctgcg ctcggagggt cagagcatgc gtgcgattgc tgccgaggtc ggggtttccg
     4381 tgggcaccgt gcactacgcg ctgaacaaga atcgaactga cgcatgaccg taacgccgca
     4441 cgatgagcat tttcttgatc gtgcaccgct tggcactacg ttcgcgtgcg gttgcacagt
     4501 gcgcgccacg ttcttatcct gcggccattg tggctacagc caatgggggg catcagcaac
     4561 ggacgttgaa cccggtgggc aagtgttact cagggggaca tgcccagtct gcggcgctcg
     4621 gattgacggt atggcagtcg tgcatgcggc cccaccgtca aactcattca ggtatcagtg
     4681 agaaccctca tggcaccccc tcgtgacacg ttctcgttgc gatcagctgc ggccaagctg
     4741 atcttcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     4801 gacccggcta ggctggcggg gttgccttac tgatcaggcc gcgttgctgg cgtttttcca
     4861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5281 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5521 gcgcctcacc aatgcttgat gagggaggca ccgatctcgg cgatctgacg gttgcgctca
     5581 tccatcgtgg cctgggagcc ggtcgtgtag atcaccacga tgcggctggg cttaccgtcg
     5641 ggaccgagag ccgcgatgat gccacgcgag ccgcgctcac cagcacccga cttgtccgcg
     5701 atgaaccaac cggccgggag cgcgctgcgc aacagcgggc cagcgacctt gtcggcctcc
     5761 atccagtcga tcagttgctg acgggaggcc agagtgagga gttcaccggt caggagttta
     5821 cggagggtgg tagccatagc cgcgggcata gtcgtgtcgc gctcgtcgtt cgggatagcc
     5881 tcattaagtt cgggttccca acggtccaga cgggtcacgt ggtcgcccat gttgtggaga
     5941 aaagccgtca gctctttcgg gccaccgatg gtcgtcaaca gcagattggc ggcagtgttg
     6001 tcggacatag tgatggccgc ggagcacagc tcgcgcacgg tcatcccatc ggtcagatgt
     6061 ttctccgtga ccggggagta ttcgaccagg tcgttctggg agtaatgaat gcgacggccc
     6121 agttgctcct gcccagcatc cacccggcta agcacagcgc cgcagaggag gaccttgaac
     6181 gtggacatca tggggaaacg ctcctcaggg cggaaagact ccagaatctt accggagttc
     6241 aggtccaact cgatataacc gacgcgcgcc cccagctgat cctcggcgtc cttgaccttg
     6301 accagggttt ccggatgggc aaaaaccggc aagcaaaagg ccgcaaaaaa tgggataagg
     6361 gcaacgcgga agtgctggat cgacatactc ttcctttttc aatattattg aagcatttat
     6421 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6481 ggggttccgc gactagttcc ccgaaaagtg ccacctgacg ttaagaaacc attattatca
     6541 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctacaa tcagcgctga
     6601 cccggaccgc cacgacggcc tggacgaccg ccggtacgac caccacccga agggccacga
     6661 cgaccaccct ggtcgccctg ctcgcgctcc ccggtacgac catcgtcgcg attacggcca
     6721 gaggaccgac ggtcaccgtg gccggaggcc tgacggtcgc gacggtcctg accacccgtc
     6781 cgacggtcgc cacgatcctg gccacccgta cggcggtcac cacgatcctg accacccgta
     6841 cgacggccgc gctgcggttg gccaccgcgc ggctcgttct tctggtcaag gcgctcgaag
     6901 agccgcagcc attgctccgg ccggacgtag gccacgacag caccaccacc cacgccggca
     6961 tggtccagcg cagcctccac gcgacgacga gggtacttgc gcaggaggga ggcttgaatg
     7021 ttgccgccga caccagtaaa acacaactcc accatgctct cgtagcgctc aagggcagca
     7081 ccctccaaca gcggttcagc gcggcgacgc agacgcatga tggcggaatc gaccttcggg
     7141 accggcttga acaagcggcg atcgaccttc tcgacgaact cccactcgaa gagcggccaa
     7201 gtcatgaccg tgaggcggga ccagcggcca taatcaccgg tgcgcttacg ggcaaattcg
     7261 agttgggtaa ccatcgtggc ggtttcgatc gtcggagcct ccaggcacca atcgacgatc
     7321 gcagacgtga tgccgtacgg gatcgcgccg acgaaggcaa acggctcggg agggggctcg
     7381 gcggtcagga agtccgcgtt gacgacctcg atattcgggt ggccggacag cttctcacgc
     7441 agggacttcg ccaagcgcgg gtcgatctcg tagctcgtca cctggcgggc ccgatcggca
     7501 agctcacggg tcagcagacc ctcgcctggg ccagcctcca ggaccggaag gtcggggcgc
     7561 agttcggccg tttccgcgat gcgggcgatc gtcttacggt cacgaaggaa attctgaccg
     7621 aattgacggc ggttgcggtc ccgctcagta cggcccagga ccgggcggtt ctggttcggg
     7681 tgctggcggt cctggttgcg gcgacgcggg cgcggttgct cgtcggacga cgacatgacg
     7741 atctcccgat gtgtcgtacg tatgggaatt gacgaaaatc atatagattt tcagttgaag
     7801 tgtagcactg catagtgtca tcgaagtgtc ggcggcaacg gtatgcccgg tagggggtgg
     7861 gtctgccttt cggtttcgtg aacctgatgc ggcgacgcgc atgtagccga taacccctag
     7921 actcgcccgt gcagatcgtc ggtgt
//
