LOCUS       sequence-451418-       10483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. 
            pBRESP36A-based vector optimized for reduced size and modular 
            assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Nevot G, Santos-Moreno J, Campama-Sanz N, Toloza L, Parra-Cid C, 
            Jansen PAM, Barbier I, Ledesma-Amaro R, van den Bogaard EH, Guell M
  TITLE     Synthetically programmed antioxidant delivery by a domesticated skin
            commensal.
  JOURNAL   Cell Syst. 2025 Feb 19;16(2):101169. doi: 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6.
  PUBMED    39919749
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-02-19"; volume: "16(2):101169. doi"; pages: " 
            10.1016/j.cels.2025.101169. Epub 2025 Feb 6"
COMMENT     Sequence Label: pEND-351_Cacnes_dCas9_CRISPRi
FEATURES             Location/Qualifiers
     source          1..10483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1296..1318
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(1326..1352,1353..1355,1356..3842,3843..3845,
                     3846..5429)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5430..5447
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      complement(7393..7981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7473..7492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8064..8855,8856..8924))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8925..9029)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9096..9114
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctagaggaa atcagagctt gaggctgccg gagcgacggt agacccgtcc ggttgggtgg
       61 tgccactgcg tgcactcaag gtcgtttttg gggtgtcaga tgtgacctcg aatgcggaat
      121 tcataggata gattctggaa actttaccgt ccgagctcag gcttacctta ctcgagcaat
      181 aaacagttga tagggcttct ccgttaccat ggttcagcca aaaaacttaa gaccgccggt
      241 cttgtccact accttgcagt aatgcggtgg acaggatcgg cggttttctt ttctcttctc
      301 aattcttctg acctgtaacg aataatagat agtaaagtag tctccgattg agttttctct
      361 gccgagtccc acccagttct gtgatttcag taagttggta attgatacac tgttgcgaga
      421 actggtgcct ggtagtagat aggttgttat tgagtaagaa ggtaaagtga acgaaatccc
      481 tgaaactgag actgtagaaa ataagcttgt ccagactatt ggatccaaga gatttctaca
      541 cgattgagca ctgtctcctg caggctcggt accaaattcc agaaaagagg cctcccgaaa
      601 ggggggcctt ttttcgtttt ggtcctaata gataaaggat aggtctggta gtgttgttcg
      661 ttctcgcagg taaatcaata atactctgca gttccgtaga cttttcagtg ggacagggta
      721 gcgataacag atagattgta ataagacaca gtaggtgctc gtagttgcgt gaagagaacc
      781 gctcaggaaa tccagtcaga agtattggta atcgttgaaa actcagtcga cgcacttact
      841 gaagacgtcc tattacactc gtcgttggaa actgaagatg cggccgcgga aacacagaaa
      901 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggtagcga acgacgagtc
      961 actgttgagg ataaatactt tctctactag ctagctgtta cacaggtcct cagcggcgcg
     1021 cccagcctgc ggtccggggt tgatctgtag tgctgccggg gccgtgggtt ccggcagcac
     1081 tgtggactac gggcgggacg cgtgccctct gtgtgagtta ctgccggtgc cctttagctc
     1141 ctttcaattt ggggagacga cgttgagaag gtatcgttaa ctgctggctt cgtgtgtgcg
     1201 gagccacaac cacgcccgca gggggtgtgg gtcgctggga cgcccgagat agccgttaca
     1261 caggtgacac ttatttcagc ctgcggtccg gtaattttgt ttaactttaa gaaggagata
     1321 tacatatgga caaaaaatac tcgatcggcc tcgcgatcgg gaccaactcc gtcggctggg
     1381 ccgtcatcac ggacgaatac aaggtcccca gcaagaagtt caaggtcctg ggtaacaccg
     1441 accgccattc catcaaaaag aacttgattg gcgctctcct cttcgactct ggtgaaacgg
     1501 ccgaggccac ccgcctgaag cgcacggccc gccgccgtta cactcgccgc aagaaccgca
     1561 tctgctacct gcaagagatt ttctccaacg agatggctaa ggtcgatgac tccttcttcc
     1621 atcgcttgga ggagtccttc ctggtcgagg aagataagaa gcacgagcgc catcccattt
     1681 tcggcaacat cgtcgatgag gtggcctacc atgagaagta tccaaccatc taccacctgc
     1741 gcaagaagct ggtcgattcc accgacaagg cggacttgcg cctcatctac ctggccctgg
     1801 cccacatgat taagttccgc ggccacttct tgatcgaagg tgacctgaac ccggacaatt
     1861 ccgacgtgga caagctcttt atccagctgg tgcagaccta caaccagctg ttcgaggaga
     1921 acccgatcaa cgcttccggc gtcgatgcca aggccatcct gtctgcccgc ctgtccaagt
     1981 ctcgccggct cgaaaacctg atcgcgcaac tgccgggcga gaagaagaac gggttgttcg
     2041 gtaacctgat cgccctgagc ctcggcctga ctcccaattt caagtccaac ttcgacctgg
     2101 ctgaagacgc caagttgcag ctctcgaagg atacctacga cgacgacctc gacaatctgc
     2161 tcgcccagat tggcgaccag tacgccgact tgttcctggc cgccaagaac ctgtccgacg
     2221 ccatcttgct gtccgacatc ctgcgcgtta ataccgagat caccaaggcc ccactttccg
     2281 cctccatgat caagcgctat gacgagcacc accaggacct caccctgctg aaggccttgg
     2341 tccgccagca actgccggaa aaatacaagg agatcttctt cgatcagtcg aagaatggct
     2401 atgccggtta catcgacggc ggcgcttccc aagaggagtt ctacaagttc attaagccta
     2461 tcctggagaa gatggacggt actgaggagc tgttggtcaa gttgaaccgt gaagacctgc
     2521 tccgcaagca gagaactttc gacaacggct ccatcccgca ccagatccat ctcggcgagc
     2581 tgcacgccat cctgcgccgc caggaggatt tctacccgtt cttgaaggac aaccgcgaga
     2641 agatcgagaa gatcctgacc ttccgcattc cgtactacgt cggcccactc gcccgcggca
     2701 actcccgttt cgcctggatg acccgcaaga gcgaagagac catcaccccc tggaatttcg
     2761 aggaggtcgt cgataagggc gcttccgccc agtcctttat cgagcgtatg accaacttcg
     2821 ataagaacct gcccaacgag aaagtcctgc ccaagcactc gctgttgtac gagtacttca
     2881 ccgtctacaa tgagctgacg aaggtgaagt acgtcactga aggcatgcgt aagccggcct
     2941 tcctgtccgg ggagcaaaag aaggccatcg tcgatctcct cttcaaaacc aaccgtaagg
     3001 tcaccgtcaa gcaacttaag gaggactatt tcaagaaaat cgagtgcttc gactccgtcg
     3061 agatctcggg cgtcgaagac cgcttcaacg cctcgctggg cacctaccac gacttgctga
     3121 agattatcaa ggacaaagat ttcctggaca acgaagagaa cgaggacatc ctcgaagaca
     3181 tcgtgctgac cctgactctc ttcgaggatc gcgagatgat cgaggagcgc ctcaagacct
     3241 acgctcacct gtttgacgac aaggtcatga agcaactgaa acgtcgtcgc tacaccggtt
     3301 gggggcgctt gtcgcgcaag ttgatcaacg gtatccgtga caagcagagc ggcaagacta
     3361 tcctggactt tcttaagtcc gacggcttcg ccaaccgcaa ctttatgcag ctgatccatg
     3421 acgactcgct gaccttcaag gaggacatcc agaaggctca ggtgtccggt caaggtgact
     3481 cgctgcacga gcacatcgcg aatcttgccg gctcccctgc catcaagaaa ggcatcctgc
     3541 agactgtcaa ggtcgtcgat gagctggtca aggtcatggg ccgtcacaag cccgagaaca
     3601 ttgtgatcga gatggcgcgc gagaaccaga ccacccaaaa gggccagaag aacagccgtg
     3661 agcgcatgaa gcggatcgag gaaggcatca aggagcttgg ttcccagatc ctgaaggagc
     3721 acccggtcga gaacacccag ctgcaaaacg agaaactgta cctttactac ctgcagaacg
     3781 gccgcgacat gtacgtggat caagagttgg acatcaaccg gctctctgac tacgacgtgg
     3841 acgccatcgt gccgcaatcc ttcctgaaag acgactcgat tgacaacaaa gtcttgaccc
     3901 gctcggacaa gaaccgcggt aagtcggaca acgtcccgtc cgaggaggtc gtcaagaaaa
     3961 tgaagaacta ctggcgtcag ctgttgaacg cgaagctgat cacgcagcgc aagttcgaca
     4021 accttaccaa ggcggaacgc ggggggctgt ccgaactgga caaggccggc ttcattaagc
     4081 gccagttggt cgagacccgc cagatcacca agcacgttgc ccagatcttg gactctcgta
     4141 tgaacaccaa gtacgacgaa aacgataagt tgatccgcga ggtgaaggtg attacgctca
     4201 agagcaagct ggtgtccgac ttccgcaagg acttccagtt ctacaaggtc cgtgagatta
     4261 ataactatca ccacgcccac gacgcctacc ttaacgccgt cgtcggtacg gccctgatca
     4321 agaagtaccc gaagttggag agcgagttcg tctacggcga ttacaaggtc tacgacgtgc
     4381 gtaagatgat cgccaagtcg gaacaagaga tcggcaaggc caccgcgaag tacttcttct
     4441 actccaacat catgaacttt ttcaagaccg aaatcactct cgccaacggg gagatccgca
     4501 agcgtccgct gatcgaaact aacggtgaaa cgggggaaat tgtctgggac aagggtcgcg
     4561 atttcgccac ggtccggaag gtgttgtcca tgccgcaagt caacattgtg aagaagacgg
     4621 aggtgcagac cggcggtttc tctaaggagt ccatcctgcc aaagcgcaac tccgacaagt
     4681 tgatcgcccg caagaaggac tgggacccga agaaatatgg cggcttcgac agcccgaccg
     4741 tggcctactc ggttcttgtg gtggcgaagg tggaaaaagg caagtcgaaa aaactgaaat
     4801 ctgtcaagga gctgttgggc atcaccatca tggagcgtag ctctttcgag aagaacccga
     4861 ttgacttctt ggaggccaaa ggttacaagg aggtcaagaa ggatcttatc atcaaactgc
     4921 ccaaatactc gctgttcgag ctggaaaacg ggcgtaagcg tatgctggcc tcggcgggcg
     4981 agctgcagaa aggcaacgag ctggctctgc cgtcgaagta cgtcaatttc ctgtacctgg
     5041 cctcccatta cgagaaactg aagggttctc ccgaggacaa cgagcagaag caactgttcg
     5101 tcgagcaaca taagcactac cttgacgaga tcattgagca gatttctgag ttctccaagc
     5161 gcgtgatcct tgcggacgcc aatctggaca aggtcctgtc cgcttacaac aagcaccgtg
     5221 acaagccaat ccgtgagcag gccgagaata tcattcacct gttcaccctg accaacctgg
     5281 gtgccccggc cgccttcaag tacttcgaca ccactatcga tcgcaagcgt tacacgtcca
     5341 ccaaggaggt gcttgatgct accctgatcc accagtctat caccggtctc tacgagaccc
     5401 gcatcgacct gtcccagctc ggcggcgacc atcatcacca tcaccactga taatcgctgg
     5461 gacgcccggg cacgcccgcc cggtcatgac gctgagttag tggcgcagct gcgctctgag
     5521 aacgagtttc gaccctgaca gccggcgagc tgttcaggcg aagatcgcac agctgcgcga
     5581 ggaactagcc gcaatgcccg aacacgcccc agccatccct tggagcaggt ggcagcgtca
     5641 ggggagtcgg gggatgtttg gcaggggatg tggaaagaga gttcgctttg ctcacatggc
     5701 tcaaccgggt aactaactga tatgggctct tcgtcgccca ctttgaacac gccgaggaat
     5761 ggaccacgct gaacgtgact cgcatgcttc actgcatgta tggattcgtt cgagactttg
     5821 ttccctgaga gctggctgcc acgcaagccg ctggcgtcag ccgagaagtc tggggcgtac
     5881 cggcacgtga ctcggcagag ggcgctggag ctgccttaca tcgaagcgaa cccgttggtc
     5941 atgcagtcct tggtcatcac cgatcgagat gcttcggatg ctgactgggc cgcagacctc
     6001 gctgggctgc cttcaccgtc ctacgtgtcc atgaaccgtg tcacgaccac cggacacatc
     6061 gtctatgcct tgaagaaccc tgtgtgtctg accgatgccg cgcggcgacg gcctatcaac
     6121 ctgctcgccc gcgtcgagca gggcctatgc gacgttctcg gcggcgatgc atcctacggg
     6181 caccggatca caaagaaccc gctcagcacc gcccatgcga ccctctgggg ccccgcagac
     6241 gcgctctacg agctgcgcgc cctcgcacac accctcgacg agatccacgc actgccggag
     6301 gcagggaacc cgcgtcgcaa cgtcacccga tcaacggtcg gccgcaacgt caccctgttc
     6361 gacaccaccc gcatgtgggc ataccgggcc gtccggcact cctggggcgg cccggtcgcc
     6421 gaatgggagc acaccgtatt cgagcacatc cacctactga acgaaacgat catcgccgac
     6481 gagttcgcca caggccccct cggcttgaac gaacttaagc acttatctcg atccatttcc
     6541 cgatgggtct ggcgcaactt cacccccgaa accttccgcg cacgccagaa agcgatcagc
     6601 ctccgtggag catccaaagg cggcaaagaa ggcggccaca aaggcggcat tgccagtggc
     6661 gcatcacggc gtgcccatac ccgtcaacag ttcttggagg gtctgtcatg accacacgtg
     6721 aacgcctccc ccgcaacggc tacagcatcg ccgccgctgc gaaaaagctc ggtgtctccg
     6781 agtccaccgt caagcggtgg acttccgagc cacgcgagga gttcgtggcc cgcgttgccg
     6841 cacgccacgc gcggattcgt gagctgcgct cggagggtca gagcatgcgt gcgattgctg
     6901 ccgaggtcgg ggtttccgtg ggcaccgtgc actacgcgct gaacaagaat cgaactgacg
     6961 catgaccgta acgccgcacg atgagcattt tcttgatcgt gcaccgcttg gcactacgtt
     7021 cgcgtgcggt tgcacagtgc gcgccacgtt cttatcctgc ggccattgtg gctacagcca
     7081 atggggggca tcagcaacgg acgttgaacc cggtgggcaa gtgttactca gggggacatg
     7141 cccagtctgc ggcgctcgga ttgacggtat ggcagtcgtg catgcggccc caccgtcaaa
     7201 ctcattcagg tatcagtgag aaccctcatg gcaccccctc gtgacacgtt ctcgttgcga
     7261 tcagctgcgg ccaagctgat cttcgggcag cgttgggtcc tggccacggg tgcgcatgat
     7321 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg atcaggccgc
     7381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7741 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7801 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7921 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7981 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8041 agggattttg gtcatgaggc gcctcaccaa tgcttgatga gggaggcacc gatctcggcg
     8101 atctgacggt tgcgctcatc catcgtggcc tgggagccgg tcgtgtagat caccacgatg
     8161 cggctgggct taccgtcggg accgagagcc gcgatgatgc cacgcgagcc gcgctcacca
     8221 gcacccgact tgtccgcgat gaaccaaccg gccgggagcg cgctgcgcaa cagcgggcca
     8281 gcgaccttgt cggcctccat ccagtcgatc agttgctgac gggaggccag agtgaggagt
     8341 tcaccggtca ggagtttacg gagggtggta gccatagccg cgggcatagt cgtgtcgcgc
     8401 tcgtcgttcg ggatagcctc attaagttcg ggttcccaac ggtccagacg ggtcacgtgg
     8461 tcgcccatgt tgtggagaaa agccgtcagc tctttcgggc caccgatggt cgtcaacagc
     8521 agattggcgg cagtgttgtc ggacatagtg atggccgcgg agcacagctc gcgcacggtc
     8581 atcccatcgg tcagatgttt ctccgtgacc ggggagtatt cgaccaggtc gttctgggag
     8641 taatgaatgc gacggcccag ttgctcctgc ccagcatcca cccggctaag cacagcgccg
     8701 cagaggagga ccttgaacgt ggacatcatg gggaaacgct cctcagggcg gaaagactcc
     8761 agaatcttac cggagttcag gtccaactcg atataaccga cgcgcgcccc cagctgatcc
     8821 tcggcgtcct tgaccttgac cagggtttcc ggatgggcaa aaaccggcaa gcaaaaggcc
     8881 gcaaaaaatg ggataagggc aacgcggaag tgctggatcg acatactctt cctttttcaa
     8941 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9001 tagaaaaata aacaaatagg ggttccgcga ctagttcccc gaaaagtgcc acctgacgtt
     9061 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9121 gtctacaatc agcgctgacc cggaccgcca cgacggcctg gacgaccgcc ggtacgacca
     9181 ccacccgaag ggccacgacg accaccctgg tcgccctgct cgcgctcccc ggtacgacca
     9241 tcgtcgcgat tacggccaga ggaccgacgg tcaccgtggc cggaggcctg acggtcgcga
     9301 cggtcctgac cacccgtccg acggtcgcca cgatcctggc cacccgtacg gcggtcacca
     9361 cgatcctgac cacccgtacg acggccgcgc tgcggttggc caccgcgcgg ctcgttcttc
     9421 tggtcaaggc gctcgaagag ccgcagccat tgctccggcc ggacgtaggc cacgacagca
     9481 ccaccaccca cgccggcatg gtccagcgca gcctccacgc gacgacgagg gtacttgcgc
     9541 aggagggagg cttgaatgtt gccgccgaca ccagtaaaac acaactccac catgctctcg
     9601 tagcgctcaa gggcagcacc ctccaacagc ggttcagcgc ggcgacgcag acgcatgatg
     9661 gcggaatcga ccttcgggac cggcttgaac aagcggcgat cgaccttctc gacgaactcc
     9721 cactcgaaga gcggccaagt catgaccgtg aggcgggacc agcggccata atcaccggtg
     9781 cgcttacggg caaattcgag ttgggtaacc atcgtggcgg tttcgatcgt cggagcctcc
     9841 aggcaccaat cgacgatcgc agacgtgatg ccgtacggga tcgcgccgac gaaggcaaac
     9901 ggctcgggag ggggctcggc ggtcaggaag tccgcgttga cgacctcgat attcgggtgg
     9961 ccggacagct tctcacgcag ggacttcgcc aagcgcgggt cgatctcgta gctcgtcacc
    10021 tggcgggccc gatcggcaag ctcacgggtc agcagaccct cgcctgggcc agcctccagg
    10081 accggaaggt cggggcgcag ttcggccgtt tccgcgatgc gggcgatcgt cttacggtca
    10141 cgaaggaaat tctgaccgaa ttgacggcgg ttgcggtccc gctcagtacg gcccaggacc
    10201 gggcggttct ggttcgggtg ctggcggtcc tggttgcggc gacgcgggcg cggttgctcg
    10261 tcggacgacg acatgacgat ctcccgatgt gtcgtacgta tgggaattga cgaaaatcat
    10321 atagattttc agttgaagtg tagcactgca tagtgtcatc gaagtgtcgg cggcaacggt
    10381 atgcccggta gggggtgggt ctgcctttcg gtttcgtgaa cctgatgcgg cgacgcgcat
    10441 gtagccgata acccctagac tcgcccgtgc agatcgtcgg tgt
//
