LOCUS       sequence-451440-        9702 bp    DNA     circular SYN 18-DEC-2025
DEFINITION  Non-integrating (episomal) expression of mouse p53DD - p53 
            carboxy-terminal dominant-negative fragment .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCE-mp53DD
FEATURES             Location/Qualifiers
     source          1..9702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..667
                     /label=chicken beta-actin promoter
     intron          668..1684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1608..1630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1692..1711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(2149..2168)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2214..2269
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2268..2287)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2630..2646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2630..2646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2643..2665)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2654..2670
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2678..2684,2685..2702,2703..2708))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2723..2744
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             3223..5148
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      5450..7239
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(7780..7797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7951..8539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8031..8050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8710..9501,9502..9570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9333..9352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9571..9675)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
      541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1441 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1501 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1561 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1621 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     1681 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcgc
     1741 ccttcaccag gatgactgcc atggaggagt cacagtcgga tatcagcctc aagagagcgc
     1801 tgcccacctg cacaagcgcc tctcccccgc aaaagaaaaa accacttgat ggagagtatt
     1861 tcaccctcaa gatccgcggg cgtaaacgct tcgagatgtt ccgggagctg aatgaggcct
     1921 tagagttaaa ggatgcccat gctacagagg agtctggaga cagcagggct cactccagct
     1981 acctgaagac caagaagggc cagtctactt cccgccataa aaaaacaatg gtcaagaaag
     2041 tggggcctga ctcagactga agggcgaatt cactcctcag gtgcaggctg cctatcagaa
     2101 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     2161 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     2221 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2281 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     2341 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     2401 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     2461 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     2521 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     2581 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     2641 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2701 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2761 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ctcaattctc atgtttgaca
     2821 gcttatcatc gataagctga tcctcacagg ccgcacccag cttttcttcc gttgccccag
     2881 tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa tccccatccc
     2941 taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct caggacgcaa
     3001 atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat gaggacgagg
     3061 atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac catgaagggg
     3121 atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat tcactgagcg
     3181 tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca
     3241 ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc agaaggctcc
     3301 ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg gggaagagga
     3361 cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg gccaagacat
     3421 agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa agggacccac
     3481 ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc aggagcagga
     3541 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg ggcaggagca
     3601 ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
     3661 ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
     3721 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
     3781 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg
     3841 gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg ggcaggaggg
     3901 gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga
     3961 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
     4021 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
     4081 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     4141 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg agcaggaggt
     4201 ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg aggccggggt
     4261 cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt
     4321 gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt
     4381 cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag gccatttttc
     4441 caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc agatggtgag
     4501 cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg agaaggccca
     4561 agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga
     4621 aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga aggtttaaga
     4681 gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg ggtcgccggt
     4741 gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg aactgccctt
     4801 gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat ggcccctgga
     4861 cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat ggtcttttta
     4921 caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt tatgacaaag
     4981 cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg agtagatttg
     5041 cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga cggagatgac
     5101 ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg taacttgtta
     5161 ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga ataaatcccc
     5221 agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca ccattttcgt
     5281 cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca
     5341 acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga
     5401 cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga
     5461 caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc
     5521 cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc
     5581 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
     5641 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
     5701 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
     5761 agatatagat taggatagca tatgctaccc agatatagat taggatagca tatgctatcc
     5821 agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata ctaccctaat
     5881 ctctattagg atagcatatg ctacccggat acagattagg atagcatata ctacccagat
     5941 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
     6001 ataaattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
     6061 atagattagg atagcctatg ctacccagat atagattagg atagcatatg ctatccagat
     6121 atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt
     6181 gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc
     6241 tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc
     6301 ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta
     6361 caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg
     6421 ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt
     6481 atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg
     6541 agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat
     6601 ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca
     6661 ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg
     6721 gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca
     6781 ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac
     6841 cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc
     6901 acctcacata caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag
     6961 aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac
     7021 tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa
     7081 ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta
     7141 attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga
     7201 tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt
     7261 ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct
     7321 ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc
     7381 ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag
     7441 tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca
     7501 tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt
     7561 gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg
     7621 cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg
     7681 gatcgatcct ctagagtcga cgctagcgga tccgctgcat taatgaatcg gccaacgcgc
     7741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gg
//
