LOCUS       sequence-451442-        5412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is for the induced expression of nrsB (formerly known 
            as ORF229) from ICP1_2001..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Woldetsadik YA, Lazinski DW, Camilli A
  TITLE     A Vibrio cholerae anti-phage system depletes nicotinamide adenine 
            dinucleotide to restrict virulent bacteriophages.
  JOURNAL   mBio. 2024 Oct 8:e0245724. doi: 10.1128/mbio.02457-24.
  PUBMED    39377576
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2024 
            Oct 8:e0245724. doi: 10.1128/mbio.02457-24."
COMMENT     Sequence Label: pDL1530
FEATURES             Location/Qualifiers
     source          1..5412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1612..2157
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2058..2077
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2274..2291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2486..2505)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2497..3312
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2497..3312
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2570..2589)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     oriT            3656..3765
                     /note="incP origin of transfer"
     regulatory      4189..4198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4201..5079)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5106..5390
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     5313..5332
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 atgaataata ttaacgcagc tctaatgaaa gatacttaca aagctttcca caagttttca
       61 tacaacccag aagtgactca cgtatatagt aacttcacta accgacacgg taagtattct
      121 aatacaaaat ctgatgaggt tgtcttcgtg ggtcttcaat atttcatcaa agaggttttg
      181 attaaacagt ggaatgaaac attcttctct aaaaccaaag aagaggctgt tggtgaatac
      241 cgtcgtgtaa ttgaaagtat gttaggtaaa cctattgaca ccaaacacat ggaagcttta
      301 catgatttag gttatctacc aatcttaatc aaggcgcttc aggaaggtag tgttgttggt
      361 taccaaatcc caacactaac aatcactaac accgtaagtg gtttcggttg ggttacaaac
      421 atgttagaga ctgtattatc gagtgaacta tggggtatct ctacatcagc cacaacagct
      481 tatgcttacc gtaaacgctt tgagaaggaa caatccctaa aagacttagg catgattcct
      541 ttccttggtc atgacttctc ttatcgtggc atgtttggta cacaagctgc tgcacaaagt
      601 ggtttcggac acttatgttc atttgtaggt agcgatacaa tcccagccgc attgtttgca
      661 gaagaatact atggtgctaa gattgataaa gagttggtat ttgctagtgt agatgcaaca
      721 gaacatagtg taatgtgttc ttatggaaca gaaggtgaac aagcttcact agaacaccta
      781 atcactaatg taacaccatc aggtattatc tctgtagtat ctgatacgtg ggatttctgg
      841 aagcttgtaa cagaatatct accaaaccta aaagacaaga ttatgtctcg tgaaggaact
      901 ttggttattc gtccagatag tggtgaccca gttaaaatcc tttgtggtga tgaagactct
      961 aaactaggtt ctgcagaaaa taaaggactc attgaatgtc tgtgggatac ctttggtgga
     1021 actatccaag agtcgacagg gttgaaactt ctagattcac atattggtgc aatctacggg
     1081 gattctatca cactagaacg ccaagatgag attatcaagc gtcttattac taaaggtttt
     1141 gttcctagtg ttgtattagg tattggtagc tacacttacc aatatgtcac tcgtgataca
     1201 cacggttctg cagttaaagc aacatgtgta caaaaaggtg aaggtaatta cgaaccaatc
     1261 ttcaaagacc ctaagacaga taagagtaag aaatctgcaa aggggttatt gatggtgaca
     1321 aaagaagaag gtaagtttgt attacattca gatgttacac cagaacaaga acaacagggt
     1381 gaacttatcc ctgtattcaa agatggtaaa cttcttgtag agacgacttt atctaagatt
     1441 cgtgaacgta tcgatagtac attctaagtc gacagatcag gcgccgcatg caaaaagacc
     1501 cttcataaat gaagggtctt ttatgatctt ataaaaaagg cgcattgatc agtgcgcctt
     1561 tttatagtca ctccgctaaa aatatattat ctgattaata agatgatctt cttgagatcg
     1621 ttttggtctg cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt
     1681 ttcgaaggtt ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca
     1741 gtcaccaaaa cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc
     1801 ctctaaatca attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg
     1861 actcaagacg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca
     1921 tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac
     1981 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     2041 acgagggagc cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2101 cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac
     2161 ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat
     2221 ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt
     2281 cagtgagcga ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgcag
     2341 ccttttttct cctgccacat gaaggacctg cagggggggg ggggggaaag ccacgttgtg
     2401 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2461 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2521 tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     2581 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat
     2641 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     2701 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     2761 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag
     2821 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     2881 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     2941 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     3001 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc
     3061 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     3121 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     3181 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     3241 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     3301 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     3361 tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatca
     3421 cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa atcaccaact
     3481 ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg ctggatgatg
     3541 gggcgattca ggcttaattc tggaaggcag tacaccttga taggtgggct gcccttcctg
     3601 gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc
     3661 cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag
     3721 aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg
     3781 atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag
     3841 gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc agcggaaaag
     3901 cgctgcttcc ctgctgtttt gtggaatatc taccgactgg aaacaggcaa atgcaggaat
     3961 taagcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg cccccccccc
     4021 ctgcaggtca cttcactgac accctcatca gtgccaacat agtaagccag taatcgaatt
     4081 ggctagtaat tggggaccct agaggtcccc ttttttattt taaaaatttt ttcacaaaac
     4141 ggtttacaag cataaagctt gctcaatcaa tcaccggatc ccagtctggc cgccatggca
     4201 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
     4261 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
     4321 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
     4381 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
     4441 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
     4501 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
     4561 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
     4621 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
     4681 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
     4741 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
     4801 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
     4861 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
     4921 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
     4981 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
     5041 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
     5101 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
     5161 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     5221 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     5281 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     5341 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     5401 ttgggaggtt at
//
