LOCUS       sequence-451443-        4283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is for correcting the nonsense mutation in the nrsB 
            gene of several ICP1 isolates..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Woldetsadik YA, Lazinski DW, Camilli A
  TITLE     A Vibrio cholerae anti-phage system depletes nicotinamide adenine 
            dinucleotide to restrict virulent bacteriophages.
  JOURNAL   mBio. 2024 Oct 8:e0245724. doi: 10.1128/mbio.02457-24.
  PUBMED    39377576
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2024 
            Oct 8:e0245724. doi: 10.1128/mbio.02457-24."
COMMENT     Sequence Label: pDL1531
FEATURES             Location/Qualifiers
     source          1..4283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      483..1028
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     929..948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1145..1162
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1357..1376)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1368..2183
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1368..2183
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1441..1460)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     oriT            2527..2636
                     /note="incP origin of transfer"
     regulatory      3060..3069
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3072..3950)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3977..4261
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4184..4203
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 gtatgtagat tatgtattct gccctaatat tgtaggtggt tatatcaacc aacaagatat
       61 ccaaaaattt aacagtaaat aagttttagt ggacacagaa aaaggagaaa cacatatgaa
      121 taatattaac gcagctctaa tgaaagatac ttacaaagct ttccacaagt tttcatacaa
      181 cccagaagtg actcacgtat atagtaactt cactaaccga cacggtaagt attctaatac
      241 aaaatctgat gaggttgtct tcgtgggtct tcaatatttc atcaaagagg ttttgattaa
      301 acagtggaat gaaacattct tctctaaaac caaagaaggt cgacagatca ggcgccgcat
      361 gcaaaaagac ccttcataaa tgaagggtct tttatgatct tataaaaaag gcgcattgat
      421 cagtgcgcct ttttatagtc actccgctaa aaatatatta tctgattaat aagatgatct
      481 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
      541 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
      601 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
      661 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
      721 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
      781 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
      841 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
      901 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
      961 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     1021 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
     1081 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
     1141 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     1201 caccggtgca gccttttttc tcctgccaca tgaaggacct gcaggggggg gggggggaaa
     1261 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1321 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     1381 aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg
     1441 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
     1501 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     1561 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     1621 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa
     1681 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     1741 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     1801 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     1861 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
     1921 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     1981 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2041 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2101 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2161 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     2221 ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgaacttttg ctgagttgaa
     2281 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa
     2341 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg
     2401 gctggatgat ggggcgattc aggcttaatt ctggaaggca gtacaccttg ataggtgggc
     2461 tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg
     2521 cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
     2581 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
     2641 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
     2701 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
     2761 cagcggaaaa gcgctgcttc cctgctgttt tgtggaatat ctaccgactg gaaacaggca
     2821 aatgcaggaa ttaagcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
     2881 gccccccccc cctgcaggtc acttcactga caccctcatc agtgccaaca tagtaagcca
     2941 gtaatcgaat tggctagtaa ttggggaccc tagaggtccc cttttttatt ttaaaaattt
     3001 tttcacaaaa cggtttacaa gcataaagct tgctcaatca atcaccggat cccagtctgg
     3061 ccgccatggc attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
     3121 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
     3181 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
     3241 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
     3301 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
     3361 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
     3421 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
     3481 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
     3541 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc
     3601 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
     3661 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
     3721 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
     3781 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
     3841 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
     3901 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
     3961 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
     4021 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
     4081 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
     4141 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
     4201 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
     4261 tacccgtttt tttgggaggt tat
//
