LOCUS       sequence-451461-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red fluorescent calcium sensor FR-GECO1c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Hayward RF, Cohen AE
  TITLE     All-optical mapping of Ca(2+) transport and homeostasis in 
            dendrites.
  JOURNAL   Cell Calcium. 2024 Dec 5;125:102983. doi: 
            10.1016/j.ceca.2024.102983.
  PUBMED    39662137
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium."; date: "2024-12-5"; volume: "125:102983. doi"; pages: " 
            10.1016/j.ceca.2024.102983"
COMMENT     Sequence Label: pLenti_CMV_FR-GECO1c
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..69
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(129..145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(129..145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(142..164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(177..183,184..201,202..207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    222..243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(360..377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(531..1119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(611..630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1290..2081,2082..2150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1913..1932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2151..2255)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2330..2349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2521..2900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2902..3100
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             3118..3298
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3345..3470
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3963..4196
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4381..4425
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4723..4840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4899..5202
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5203..5406
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5403..5427
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5490..5499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6762..7350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6815..6835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7233..7244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7353..7369)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7354..7370)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7875..8055
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8081..8098)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8087..8311
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8357..8785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8444..8463)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8654..8675
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8794..8814)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8799..9128
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8979..9114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9041..9060
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9176..9223
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9242..9616
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    join(9746..9787,1..80)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(join(9783..9787,1..15))
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
       61 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
      121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2281 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2341 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2401 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2461 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     2521 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2581 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2641 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2701 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2761 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2821 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2881 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2941 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     3001 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     3061 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     3121 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3181 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3241 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3301 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     3361 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     3421 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     3481 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     3541 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     3601 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     3661 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     3721 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     3781 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3841 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3901 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3961 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     4021 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     4081 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     4141 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     4201 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     4261 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     4321 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     4381 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     4441 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     4501 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     4561 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     4621 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     4681 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     4741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     4801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4861 cagagatcca gtttggttaa ttaagcttgg gagttccgcg ttacataact tacggtaaat
     4921 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4981 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagca tttacggtaa
     5041 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     5101 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     5161 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     5221 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     5281 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     5341 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5401 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     5461 catagaagac accgactcta gaggatcccg ccaccatggg cagcgtgaag cagatcccta
     5521 gcctgaccac agtgatcctg gtcaagtcca tgctgcggaa gcggagcttc ggcaacccct
     5581 tcggttttct gaccgagacc ctggtgcctg tggacggtgg cctggcaggc acatgtgaca
     5641 tggccctgag actcgttggc ggcggacacc tgaagtgcaa cctgaaaacc acctacagaa
     5701 gcaagaagcc cgccaagaac ctgaagatgc ctggcgaaca ccaggtgggc cggaagctgg
     5761 aaagaatcaa agaggccgac aacgagacat acgtggaaca gcacgaagtg gccgtggcca
     5821 gatatgttgg acttggcgga ggcggaggaa caggcggatc tgtgtctgag ctgatcaaag
     5881 aaaacatgcg gatgaagctc tacttggaag ggaccgtcgg caaccaccac ttcaagtgta
     5941 ctgccgaagg cgagggcaag ccctatgagg gaacacagac ccagcggatc aaggtggtgg
     6001 aaggtggccc tctgcctttc gcctttgaca tcctggccac ctgttttatg tacggcagca
     6061 agaccttcat caactatccc cagggcatcc ccgatttctt caagcagagc ttccccgagg
     6121 gcttcacctg ggagagagtg accacatacg aggatggcgg cgtgctgacc gtgacacagg
     6181 atacaagcct ccaggacggc tacctgatct acaatgtgaa gctgctgggc gtgaacttcc
     6241 ccagcgatgg acccgtgatg cagaagaaaa ccctcggctg ggtgcccacc gatggcacgc
     6301 tgacagaaga actgatcgcc gagttcaaag aagccttcag cctgttcgac aaggacggcg
     6361 acggcaccat caccaccaaa gaactgggct ccgtgatgcg gagcctgggc cagaatccaa
     6421 cagaagccga actccaggac atgatcaacg aggtggacgc cgatggcgac ggaaccttcg
     6481 attaccctga gttcctgacc atgatgtccc ggaagatgag ctacagagtg accgaggaag
     6541 agatccggga agccttccag gttttcgata aggatggcaa cggctacatc ggagccgccg
     6601 aactgagacg cgtgatggcc gatctgggcg agaagctgac tgacgaagag gttgacgaga
     6661 tgatcagagt ggccgacatc gacggcgacg gacaagtgaa ctacgaagag ttcgtgcaga
     6721 tgatgaccgc caagtaggaa ttcgatatca agcttatcga taatcaacct ctggattaca
     6781 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6841 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6901 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6961 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7021 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7081 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7141 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7201 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7261 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7321 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     7381 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7441 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     7501 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7561 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     7621 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     7681 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     7741 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     7801 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     7861 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7921 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7981 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8041 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     8101 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     8161 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     8221 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     8281 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     8341 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8401 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8461 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8521 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     8581 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8641 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     8701 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8761 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     8821 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8881 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8941 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9001 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9061 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9121 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     9181 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     9241 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     9301 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     9361 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     9421 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     9481 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     9541 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     9601 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     9661 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     9721 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     9781 aagcaat
//
