LOCUS       sequence-451462-       10168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CapChR2-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Hayward RF, Cohen AE
  TITLE     All-optical mapping of Ca(2+) transport and homeostasis in 
            dendrites.
  JOURNAL   Cell Calcium. 2024 Dec 5;125:102983. doi: 
            10.1016/j.ceca.2024.102983.
  PUBMED    39662137
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Calcium."; date: "2024-12-5"; volume: "125:102983. doi"; pages: " 
            10.1016/j.ceca.2024.102983"
COMMENT     Sequence Label: pLenti_CMV_CapChR2-GFP
FEATURES             Location/Qualifiers
     source          1..10168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    25..613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(78..98)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(496..507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(616..632)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(617..633)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1138..1318
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1344..1361)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1350..1574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1620..2048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1707..1726)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1917..1938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2057..2077)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2062..2391
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2242..2377
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2304..2323
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2439..2486
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2505..2879
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3009..3130
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3046..3065)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3100..3119
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3179..3195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3179..3195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3192..3214)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3203..3219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3227..3233,3234..3251,3252..3257))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3272..3293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3410..3427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3581..4169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3661..3680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4340..5131,5132..5200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4963..4982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5201..5305)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5380..5399)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5571..5950
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5952..6150
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6168..6348
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6395..6520
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7013..7246
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7431..7475
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7773..7890
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7949..8252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8253..8456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             8570..9433
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFDIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVICIHLSNLTGLKDDYSKRTMRLLVSDVGCIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKECWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     CDS             join(9452..9454,9455..10168)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9452..9454,9455..10165)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9494..9515)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9755..9774)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10102..10123
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
       61 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
      121 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
      181 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
      241 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
      301 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
      361 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
      421 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
      481 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
      541 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
      601 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
      661 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
      721 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
      781 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
      841 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
      901 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
      961 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     1021 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     1081 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     1141 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1201 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1261 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     1321 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1381 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1441 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1501 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     1561 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     1621 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1681 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1741 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1801 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1861 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1921 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     1981 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2041 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     2101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     2161 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     2221 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2281 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     2341 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     2401 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     2461 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     2521 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     2581 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     2641 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     2701 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     2761 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     2821 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     2881 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     2941 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     3001 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3061 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3121 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     3181 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3241 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     3301 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     3361 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3421 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3481 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3541 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3601 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3661 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3721 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3781 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3841 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3901 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3961 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4021 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4081 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4141 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4201 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4261 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4921 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     5341 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     5401 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     5461 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     5521 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     5581 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     5641 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     5701 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     5761 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     5821 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     5881 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5941 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     6001 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     6061 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     6121 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
     6181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     6361 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     6421 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     6481 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     6541 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     6601 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     6661 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     6721 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     6781 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     6841 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     6901 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6961 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7021 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7081 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7141 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7201 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7261 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7321 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7381 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7441 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7501 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7561 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7621 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     7681 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     7741 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     7801 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     7861 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     7921 gtttggttaa ttaagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7981 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     8041 cgccaatagg gactttccat tgacgtcaat gggtggagca tttacggtaa actgcccact
     8101 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     8161 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     8221 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     8281 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     8341 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     8401 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     8461 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     8521 accgactcta gagggtttag tgaaccgtca gatccgctag cgctaccgga tgctgggaaa
     8581 cggcagcgcc attgtgccta tcgaccagtg cttttgcctg gcttggaccg acagcctggg
     8641 aagcgataca gagcagctgg tggccaacat cctccagtgg ttcgccttcg gcttcgacat
     8701 cctgatcctg atgttctacg cctaccagac ttggagagcc acttgcggtt gggaggaggt
     8761 ctacgtctgt tgcgtcgagc tgaccaaggt catcatcgag ttcttccacg agttcgacga
     8821 ccccagcatg ctgtacctgg ctaacggaca ccgagtccag tggctgagat acgcagagtg
     8881 gctgctgact tgtcccgtca tctgcatcca cctgagcaac ctgaccggcc tgaaggacga
     8941 ctacagcaag cggaccatga ggctgctggt gtcagacgtg ggatgcatcg tgtggggagc
     9001 tacaagcgcc atgagcacag gctacgtcaa ggtcatcttc ttcgtgctgg gttgcatcta
     9061 cggcgccaac accttcttcc acgccgccaa ggtgtatatc gagagctacc acgtggtgcc
     9121 aaagggcaga cctagaaccg tcgtgcggat catggcttgg ctgttcttcc tgtcttgggg
     9181 catgttcccc gtgctgttcg tcgtgggacc agaaggattc gacgccatca gcgtgtacgg
     9241 ctctaccatt ggccacacca tcatcgacct catgagcaag gagtgttggg gcctgctggg
     9301 acactatctg agagtgctga tccaccagca catcatcatc tacggcgaca tccggaagaa
     9361 gaccaagatc aacgtggccg gcgaggagat ggaagtggag accatggtgg accaggagga
     9421 cgaggagaca gtggcggcac cggtagtagc agtgagcaag ggcgaggagc tgttcaccgg
     9481 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     9541 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tttgcaccac
     9601 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     9661 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     9721 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     9781 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     9841 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     9901 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     9961 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
    10021 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
    10081 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
    10141 tctcggcatg gacgagctgt acaagtaa
//
