LOCUS       sequence-451465-        6954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express human FBXO22 in insect cells, such as Trichoplusia ni Hi5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, 
            Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, 
            Guttman M, Pagano M, Zheng N
  TITLE     Recognition of BACH1 quaternary structure degrons by two F-box 
            proteins under oxidative stress.
  JOURNAL   Cell. 2024 Oct 30:S0092-8674(24)01188-7. doi: 
            10.1016/j.cell.2024.10.012.
  PUBMED    39504958
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Oct 30:S0092-8674(24)01188-7. doi: 10.1016/j.cell.2024.10.012."
COMMENT     Sequence Label: pFB-GST-FBXO22
FEATURES             Location/Qualifiers
     source          1..6954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    348..482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(385..404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     439..458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  824..989
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1173..1627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1260..1279)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1469..1490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1653..1757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1758..1826,1827..2618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1976..1995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2789..3377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3278..3297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3531..3548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3682..3906
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(3973..4506)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4676..4681,4682..4698,4699..4704))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        5082..5173
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5098..5115
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5216..5869
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     5827..5849
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             5876..5896
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cttattcggc ttctttggaa atggagaaat tggatgtgat cggatagtca ctgggaactt
       61 tatattgagg aaatgtaatg aggtaaaaga tgatgatctg tttcatagct atacaacaat
      121 aatggcactc atacatctgg ggtcatctaa ataaccggag gatgagtgtg gcggccgctt
      181 tcgaatctag agcctgcagt ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga
      241 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
      301 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      361 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
      481 tctgatcact gcttgagtaa tcccaccact cctctccact taattaacct agaggccgcg
      541 ctggcctctg cgaagaagat tgtgttgaag agttccgatg gtgaatcttt cgaggttgag
      601 gaggcggtgg ctctcgagtc acaaaccata gcgcatatgg ttgaagacga ctgcgtcgac
      661 aacggagtcc ctcttcctaa cgtcacgagc aagatcctcg ccaaggtgat cgagtattgc
      721 aagaggcacg tcgaggctgc tgcctctaag gccgaggccg tcgagggtgc tgctacctcc
      781 gatgacgatc ttaaggcctg gcctgggcgg cctaggagat ccgaaccaga taagtgaaat
      841 ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg ctacacccag
      901 ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt tccacccctc
      961 ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt tatgttttta
     1021 atcaaacatc ctgccaactc catgtgacaa accgtcatct tcggctactt tttctctgtc
     1081 acagaatgaa aatttttctg tcatctcttc gttattaatg tttgtaattg actgaatatc
     1141 aacgcttatt tgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     1201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     1321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     1381 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     1441 tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc
     1501 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     1561 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     1621 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1981 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2461 ccggtgagcg tggctctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3541 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3601 tttcacaccg catagaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
     3661 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
     3721 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
     3781 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
     3841 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ccaagggcat ggtaaagact
     3901 atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc
     3961 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
     4021 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
     4081 gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc
     4141 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg
     4201 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
     4261 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
     4321 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
     4381 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
     4441 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca
     4501 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     4561 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     4621 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     4681 acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg
     4741 tcacccggca accttgggca gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga
     4801 gcgcaaggtt tcgctctcca cgcatcgtca ggcattggcg gccttgctgt tcttctacgg
     4861 caaggtgctg tgcacggatc tgccctggct tcaggagatc ggaagacctc ggccgtcgcg
     4921 gcgcttgccg gtggtgctga ccccggatga agtggttcgc atcctcggtt ttctggaagg
     4981 cgagcatcgt ttgttcgccc aggactctag ctatagttct agtggttggc tacgtattaa
     5041 ttaaggatgg aagggtgtgt aggttaccgg aatattaata gatcatggag ataattaaaa
     5101 tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa
     5161 aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg gatctatgtc
     5221 ccctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga
     5281 atatcttgaa gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg
     5341 aaacaaaaag tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga
     5401 tgttaaatta acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt
     5461 gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat
     5521 tagatacggt gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt
     5581 tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata
     5641 tttaaatggt gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt
     5701 tttatacatg gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg
     5761 tattgaagct atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc
     5821 tttgcagggc tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatgaaaa
     5881 cctctacttc caatcgatgg agccggtagg ctgctgcggc gagtgccgcg gctcctccgt
     5941 agacccgcgg agcaccttcg tgttgagtaa cctggcggag gtggtggagc gtgtgctcac
     6001 cttcctgccc gccaaggcgt tgctgcgggt ggcctgcgtg tgccgcttat ggagggagtg
     6061 tgtgcgcaga gtattgcgga cccatcggag cgtaacctgg atctccgcag gcctggcgga
     6121 ggccggccac ctggaggggc attgcttggt tcgcgtggta gcagaggagc ttgagaatgt
     6181 tcgcatctta ccacatacag ttctttacat ggctgattca gaaactttca ttagtctgga
     6241 agagtgtcgt ggccataaga gagcaaggaa aagaactagt atggaaacag cacttgccct
     6301 tgagaagcta ttccccaaac aatgccaagt ccttgggatt gtgaccccag gaattgtagt
     6361 gactccaatg ggatcaggta gcaatcgacc tcaggaaata gaaattggag aatctggttt
     6421 tgctttatta ttccctcaaa ttgaaggaat aaaaatacaa ccctttcatt ttattaagga
     6481 tccaaagaat ttaacattag aaagacatca actcactgaa gtaggtcttt tagataaccc
     6541 tgaacttcgt gtggtccttg tctttggtta taattgctgt aaggtgggag ccagtaatta
     6601 tctgcagcaa gtagtcagca ctttcagtga tatgaatatc atcttggctg gaggccaggt
     6661 ggacaacctg tcatcactga cttctgaaaa gaaccctctg gatattgatg cctcgggtgt
     6721 ggttggactg tcatttagtg gacaccgaat ccagagtgcc actgtgctcc tcaacgagga
     6781 cgtcagtgat gagaagactg ctgaggctgc gatgcagcgc ctcaaagcgg ccaacattcc
     6841 agagcataac accattggct tcatgtttgc atgcgttggc aggggctttc agtattacag
     6901 agccaagggg aatgttgagg ctgatgcatt tagaaagttt tttcctagtg ttcc
//
