LOCUS       sequence-451472-        8481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To check the split GFP signals in mitochondria (1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8481)
  AUTHORS   Lin RZ, Im GB, Luo AC, Zhu Y, Hong X, Neumeyer J, Tang HW, Perrimon 
            N, Melero-Martin JM
  TITLE     Mitochondrial transfer mediates endothelial cell engraftment through
            mitophagy.
  JOURNAL   Nature. 2024 May;629(8012):660-668. doi: 10.1038/s41586-024-07340-0.
            Epub 2024 May 1.
  PUBMED    38693258
REFERENCE   2  (bases 1 to 8481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07340-0"; journalName: "Nature"; date: 
            "2024-05"; volume: "629"; issue: "8012"; pages: "660-668"
COMMENT     Sequence Label: PB-GFP1-10-HygR
FEATURES             Location/Qualifiers
     source          1..8481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      418..1006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     907..926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1160..1177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1294..1315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1330..1335,1336..1353,1354..1360)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1368..1384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1373..1395
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1392..1408
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1392..1408
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1429..1447
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1596..1630
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1907..1927
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(2136..3161)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2136..3161)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(3180..3679)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3228..3247)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3621..3644
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    3702..3735
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3735..3759
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        3763..4142
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4458..5474
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5398..5420
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5482..5501
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(5529..5553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5559..5633
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             5667..6308
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     polyA_signal    6312..6536
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6537..6570
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6571..6591)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     repeat_region   6970..7032
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7098..7117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7099..7117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7124..7141)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7124..7140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7133..7155)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7282..7737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7369..7388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7579..7600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7763..7867
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7868..7936,7937..8481,1..247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8086..8105)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
       61 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
      181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
      241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
      301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
      361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
      421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
      481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
      541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
      601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
      661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
      721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagagag
      841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
      901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
      961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1201 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1261 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     1321 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     1381 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca
     1441 ctaaagggaa caaaagctgg tacctcgcgc gacttggttt gccattcttt agcgcgcgtc
     1501 gcgtcacaca gcttggccac aatgtggttt ttgtcaaacg aagattctat gacgtgttta
     1561 aagtttaggt cgagtaaagc gcaaatcttt tttaacccta gaaagatagt ctgcgtaaaa
     1621 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
     1681 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
     1741 taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct
     1801 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc atgttctact
     1861 tacgtgataa cttattatat atatattttc ttgttataga tatcatcaac tttgtataga
     1921 aaagttgcaa ctatgaaaca ttatcataaa gcaatagtaa agagccttca gtaaagagca
     1981 ggcatttatc taatcccacc ccacccccac ccccgtagct ccaatccttc cattcaaaat
     2041 gtaggtactc tgttctcacc cttcttaaca aagtatgaca ggaaaaaact tccatgtgga
     2101 catctttatt gtttaataga tcatcaattt ctcgactatt cctttgccct cggacgagtg
     2161 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     2221 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     2281 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     2341 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc
     2401 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     2461 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     2521 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     2581 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     2641 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     2701 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     2761 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc
     2821 ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
     2881 cggcgggaga tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga
     2941 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     3001 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     3061 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     3121 aacttctcga cagacgtcgc ggtgagttca ggctttttca tggtggcggc tggatcggtc
     3181 gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg
     3241 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg
     3301 agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc
     3361 tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc
     3421 tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc
     3481 ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca
     3541 aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca
     3601 cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg
     3661 aaaagcgcct cccctacccg gtagaattca tcgatgatat cataacttcg tataatgtat
     3721 gctatacgaa gttatcaagt ttgtacaaaa aagcaggctg tcgacattga ttattgacta
     3781 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     3841 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3901 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3961 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     4021 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     4081 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     4141 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     4201 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     4261 ggggggcggg cgacgggggg cggggggggg gggggggggg gcgcgcgccg ggcggggcgg
     4321 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     4381 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
     4441 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
     4501 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     4561 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
     4621 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
     4681 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
     4741 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     4801 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     4861 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     4921 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     4981 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     5041 gggtgccggg cggggcgggg ccgcctcggg ccgggggggg ctcgggggag gggcgcggcg
     5101 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     5161 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     5221 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     5281 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     5341 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     5401 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     5461 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     5521 aagaattcac ccagctttct tgtacaaagt gggccaccat gtccgtcctg acgccgctgc
     5581 tgctgcgggg cttgacaggc tcggcccggc ggctcccagt gccgcgcgcc aagatccatt
     5641 cgttggggga tccaccggtc gccaccatgt ccaaaggaga agaactgttt accggtgttg
     5701 tgccaatttt ggttgaactc gatggtgatg tcaacggaca taagttctca gtgagaggcg
     5761 aaggagaagg tgacgccacc attggaaaat tgactcttaa attcatctgt actactggta
     5821 aacttcctgt accatggccg actctcgtaa caacgcttac gtacggagtt cagtgctttt
     5881 cgagataccc agaccatatg aaaagacatg acttttttaa gtcggctatg cctgaaggtt
     5941 acgtgcaaga aagaacaatt tcgttcaaag atgatggaaa atataaaact agagcagttg
     6001 ttaaatttga aggagatact ttggttaacc gcattgaact gaaaggaaca gattttaaag
     6061 aagatggtaa tattcttgga cacaaactcg aatacaattt taatagtcat aacgtataca
     6121 tcactgctga taagcaaaag aacggaatta aagcgaattt cacagtacgc cataatgtag
     6181 aagatggcag tgttcaactt gccgaccatt accaacaaaa cacccctatt ggagacggtc
     6241 cggtacttct tcctgataat cactacctct caacacaaac agtcctgagc aaagatccaa
     6301 atgaaaaata gctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     6361 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     6421 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6481 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggataa
     6541 cttcgtataa tgtatgctat acgaagttat caactttatt atacatagtt gacctcgacc
     6601 tgcagcccaa gcttggatcc ctcgagttaa ttaacgagag cataatattg atatgtgcca
     6661 aagttgtttc tgactgacta ataagtataa tttgtttcta ttatgtatag gttaagctaa
     6721 ttacttattt tataatacaa catgactgtt tttaaagtac aaaataagtt tatttttgta
     6781 aaagagagaa tgtttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt
     6841 ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt
     6901 gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc
     6961 gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt
     7021 atctttctag ggttaaataa tagtttctaa tttttttatt attcagcctg ctgtcgtgaa
     7081 taccgagctc caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac
     7141 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7201 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7261 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7321 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7381 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7441 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7501 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7561 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7621 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7681 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     7741 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7801 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7861 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7921 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7981 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8041 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8101 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8161 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8221 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8281 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8341 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8401 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8461 tctagcttcc cggcaacaat t
//
