LOCUS       sequence-451473-        8488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To check the split GFP signals in mitochondria (2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Lin RZ, Im GB, Luo AC, Zhu Y, Hong X, Neumeyer J, Tang HW, Perrimon 
            N, Melero-Martin JM
  TITLE     Mitochondrial transfer mediates endothelial cell engraftment through
            mitophagy.
  JOURNAL   Nature. 2024 May;629(8012):660-668. doi: 10.1038/s41586-024-07340-0.
            Epub 2024 May 1.
  PUBMED    38693258
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07340-0"; journalName: "Nature"; date: 
            "2024-05"; volume: "629"; issue: "8012"; pages: "660-668"
COMMENT     Sequence Label: PB-GFP11x7-HygR
FEATURES             Location/Qualifiers
     source          1..8488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..64)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     255..274
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     488..510
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     502..519
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     503..519
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     526..545
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        526..544
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   611..673
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1052..1072
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    complement(1073..1106)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1107..1331
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1365..1412)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(1458..1505)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(1551..1598)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(1644..1691)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(1737..1784)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(1830..1877)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(1923..1970)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(2007..2081)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     protein_bind    2087..2111
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2139..2158)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2166..3182
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(2220..2242)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        3508..3887
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(3891..3915)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3915..3948)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3971..4470
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4006..4029)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4403..4422
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4489..5514
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4489..5514
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(5723..5743)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     repeat_region   6020..6054
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6203..6221)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6242..6258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6242..6258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6255..6277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6266..6282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6290..6296,6297..6314,6315..6320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6335..6356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6473..6490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6644..7232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6724..6743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7403..8194,8195..8263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8026..8045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8264..8368)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(8394..8488,1..361))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
       61 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
      121 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
      181 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
      241 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
      301 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
      361 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
      421 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
      481 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
      541 tagggcgaat tggagctcgg tattcacgac agcaggctga ataataaaaa aattagaaac
      601 tattatttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta
      661 aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga
      721 tattaagttt tattatattt acacttacat actaataata aattcaacaa acaatttatt
      781 tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
      841 aacttttaaa cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca
      901 tgttgtatta taaaataagt aattagctta acctatacat aatagaaaca aattatactt
      961 attagtcagt cagaaacaac tttggcacat atcaatatta tgctctcgtt aattaactcg
     1021 agggatccaa gcttgggctg caggtcgagg tcaactatgt ataataaagt tgataacttc
     1081 gtatagcata cattatacga agttatccat agagcccacc gcatccccag catgcctgct
     1141 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     1201 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     1261 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     1321 agaaggcaca gctacatgaa tttcccgccg gacccaccac tcccggtgat accggcagca
     1381 ttgacatatt cgtgcaggac catatggtcc cgtgagctac tgccgccgct gccgctactt
     1441 ccaccactgc taccgcctgt gattccagca gcgttcacgt actcatggag taccatgtga
     1501 tccctgctac ctcctgatcc agaaccagac cctcctgaac ctcctgaccc agtaatgcca
     1561 gctgcgttta cgtattcgtg aagcaccata tggtctcggg acccgcttcc tcctgaactg
     1621 ccgctacctg agcctcccga accagttata cccgccgcgt tcacatactc atgcagcacc
     1681 atgtgatccc gagaacttcc actacttcct cctccgcttg agccacttcc ggatccggtt
     1741 attccggctg cattgacata ttcatgtagt accatatggt cgcgactgct acctccagag
     1801 ccgctcccgc tgccagatcc accactgcca gtgatgcctg cggcgttaac gtattcgtgg
     1861 agaaccatat gatctctcga actgccgcca cccgagcctc cacctgaact tccagagcca
     1921 cctgtaatcc cagcagcatt tacatactca tgaaggacca tgtggtcacg catggtggcg
     1981 accggtggat cccccaacga atggatcttg gcgcgcggca ctgggagccg ccgggccgag
     2041 cctgtcaagc cccgcagcag cagcggcgtc aggacggaca tggtggccca ctttgtacaa
     2101 gaaagctggg tgaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc
     2161 aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg
     2221 gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc
     2281 cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga cgcacgaagg
     2341 ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg cccgctagag
     2401 ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa aggaagtccc
     2461 tgcgccctct cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag
     2521 ccgccggcgc tccgggggcc gccgcgcccc tcccccgagc cctccccggc ccgaggcggc
     2581 cccgccccgc ccggcacccc cacctgccgc caccccccgc ccggcacggc gagccccgcg
     2641 ccacgccccg tacggagccc cgcacccgaa gccgggccgt gctcagcaac tcggggaggg
     2701 gggtgcaggg ggggttgcag cccgaccgac gcgcccacac cccctgctca cccccccacg
     2761 cacacacccc gcacgcagcc tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg
     2821 cccccggccg cgctcccctc gcgcacactg cggagcgcac aaagccccgc gccgcgcccg
     2881 cagcgctcac agccgccggg cagcgcggag ccgcacgcgg cgctccccac gcacacacac
     2941 acgcacgcac cccccgagcc gctccccccg cacaaagggc cctcccggag cccctcaagg
     3001 ctttcacgca gccacagaaa agaaacaagc cgtcattaaa ccaagcgcta attacagccc
     3061 ggaggagaag ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg
     3121 ggcgggcggc gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact
     3181 cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa
     3241 ggaaactttc ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg
     3301 ccccgcccct cgccccgccc ccgccccccc cccccgccca cgcgcccccg cccccccgcc
     3361 ccgcgccgcc cccgccagga gcgccccccc cccccccccc gcccccatcg ctgcacaaaa
     3421 taattaaaaa ataaataaat acaaaattgg gggtggggag gggggggaga tggggagagt
     3481 gaagcagaac gtggggctca cctcgaccat ggtaatagcg atgactaata cgtagatgta
     3541 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     3601 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     3661 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     3721 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     3781 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa
     3841 tgaccccgta attgattact attaataact agtcaataat caatgtcgac agcctgcttt
     3901 tttgtacaaa cttgataact tcgtatagca tacattatac gaagttatga tatcatcgat
     3961 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     4021 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     4081 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     4141 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     4201 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     4261 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     4321 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     4381 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     4441 ttctcctctt cctcatctcc gggcctttcg accgatccag ccgccaccat gaaaaagcct
     4501 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     4561 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     4621 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     4681 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     4741 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     4801 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     4861 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     4921 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     4981 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     5041 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     5101 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     5161 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     5221 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     5281 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5341 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     5401 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     5461 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagtcgaga
     5521 aattgatgat ctattaaaca ataaagatgt ccacatggaa gttttttcct gtcatacttt
     5581 gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg
     5641 tgggggtggg gtgggattag ataaatgcct gctctttact gaaggctctt tactattgct
     5701 ttatgataat gtttcatagt tgcaactttt ctatacaaag ttgatgatat ctataacaag
     5761 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     5821 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     5881 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     5941 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     6001 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa
     6061 aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa
     6121 aaccacattg tggccaagct gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg
     6181 cgcgaggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
     6241 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6301 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6361 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6421 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6481 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6541 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6601 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6661 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6721 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6781 tgccgcttac cggatacctg tccgcctttc tctcttcggg aagcgtggcg ctttctcata
     6841 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6901 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6961 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7021 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7081 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7141 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7201 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7261 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7321 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7381 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7441 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7501 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7561 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7621 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7681 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7741 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7801 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7861 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7921 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7981 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8041 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8101 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8161 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8221 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8281 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8341 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     8401 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8461 ccaataggcc gaaatcggca aaatccct
//
