LOCUS       sequence-451478-        8566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous expression plasmid, contains CAG promoter (CMV immediate 
            early enhancer and chicken beta actin promoter), Cas13d, and split 
            GFP(1-10) reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Kim M, Hutchins EJ
  TITLE     CRISPR-Cas13d as a molecular tool to achieve targeted gene 
            expression knockdown in chick embryos.
  JOURNAL   Dev Biol. 2024 Nov 30:S0012-1606(24)00265-3. doi: 
            10.1016/j.ydbio.2024.11.013.
  PUBMED    39622311
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2024 Nov 30:S0012-1606(24)00265-3. doi: 
            10.1016/j.ydbio.2024.11.013."
COMMENT     Sequence Label: pCAG-Cas13d-T2A-GFP(1-10)
FEATURES             Location/Qualifiers
     source          1..8566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1167..1546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1816..2832
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2756..2778
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2840..2859
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2967..5867
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="MIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDS
                     IRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLE
                     KRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTV
                     YTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYII
                     NYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITN
                     ELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRK
                     DMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFV
                     INLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPA
                     GRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAF
                     FKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDE
                     NGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQ
                     KKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRE
                     NAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLE
                     EKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKK
                     AEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVH
                     AYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCV
                     PFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5874..5897
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5904..5957
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5964..6605
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     primer_bind     complement(6720..6739)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6785..6840
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6839..6858)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7231..7247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7231..7247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7244..7266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7255..7271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7279..7285,7286..7303,7304..7309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7324..7345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7404..7599
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7450..7585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7605..7739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7642..7661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7696..7715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7807..7824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7978..8566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8058..8077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc
     1201 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1261 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1321 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1381 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1441 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1501 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1561 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     1621 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggg ggggggcgcg
     1681 cgccaggcgg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     1741 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     1801 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     1861 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     1921 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
     1981 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
     2041 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
     2101 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     2161 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     2221 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     2281 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     2341 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     2401 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     2461 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     2521 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     2581 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     2641 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     2701 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     2761 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     2821 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     2881 gaattgctcg agagttcgaa gccttaagta cgcgtaggcg cgccttaatt aacgtttaaa
     2941 catgcatgca aatggctagc gccaccatga tcgagaagaa gaagtccttc gccaagggca
     3001 tgggcgtgaa gtccacactc gtgtccggct ccaaagtgta catgacaacc ttcgccgaag
     3061 gcagcgacgc caggctggaa aagatcgtgg agggcgacag catcaggagc gtgaatgagg
     3121 gcgaggcctt cagcgctgaa atggccgata aaaacgccgg ctataagatc ggcaacgcca
     3181 aattcagcca tcctaagggc tacgccgtgg tggctaacaa ccctctgtat acaggacccg
     3241 tccagcagga tatgctcggc ctgaaggaaa ctctggaaaa gaggtacttc ggcgagagcg
     3301 ctgatggcaa tgacaatatt tgtatccagg tgatccataa catcctggac attgaaaaaa
     3361 tcctcgccga atacattacc aacgccgcct acgccgtcaa caatatctcc ggcctggata
     3421 aggacattat tggattcggc aagttctcca cagtgtatac ctacgacgaa ttcaaagacc
     3481 ccgagcacca tagggccgct ttcaacaata acgataagct catcaacgcc atcaaggccc
     3541 agtatgacga gttcgacaac ttcctcgata accccagact cggctatttc ggccaggcct
     3601 ttttcagcaa ggagggcaga aattacatca tcaattacgg caacgaatgc tatgacattc
     3661 tggccctcct gagcggactg aggcactggg tggtccataa caacgaagaa gagtccagga
     3721 tctccaggac ctggctctac aacctcgata agaacctcga caacgaatac atctccaccc
     3781 tcaactacct ctacgacagg atcaccaatg agctgaccaa ctccttctcc aagaactccg
     3841 ccgccaacgt gaactatatt gccgaaactc tgggaatcaa ccctgccgaa ttcgccgaac
     3901 aatatttcag attcagcatt atgaaagagc agaaaaacct cggattcaat atcaccaagc
     3961 tcagggaagt gatgctggac aggaaggata tgtccgagat caggaaaaat cataaggtgt
     4021 tcgactccat caggaccaag gtctacacca tgatggactt tgtgatttat aggtattaca
     4081 tcgaagagga tgccaaggtg gctgccgcca ataagtccct ccccgataat gagaagtccc
     4141 tgagcgagaa ggatatcttt gtgattaacc tgaggggctc cttcaacgac gaccagaagg
     4201 atgccctcta ctacgatgaa gctaatagaa tttggagaaa gctcgaaaat atcatgcaca
     4261 acatcaagga atttagggga aacaagacaa gagagtataa gaagaaggac gcccctagac
     4321 tgcccagaat cctgcccgct ggccgtgatg tttccgcctt cagcaaactc atgtatgccc
     4381 tgaccatgtt cctggatggc aaggagatca acgacctcct gaccaccctg attaataaat
     4441 tcgataacat ccagagcttc ctgaaggtga tgcctctcat cggagtcaac gctaagttcg
     4501 tggaggaata cgcctttttc aaagactccg ccaagatcgc cgatgagctg aggctgatca
     4561 agtccttcgc tagaatggga gaacctattg ccgatgccag gagggccatg tatatcgacg
     4621 ccatccgtat tttaggaacc aacctgtcct atgatgagct caaggccctc gccgacacct
     4681 tttccctgga cgagaacgga aacaagctca agaaaggcaa gcacggcatg agaaatttca
     4741 ttattaataa cgtgatcagc aataaaaggt tccactacct gatcagatac ggtgatcctg
     4801 cccacctcca tgagatcgcc aaaaacgagg ccgtggtgaa gttcgtgctc ggcaggatcg
     4861 ctgacatcca gaaaaaacag ggccagaacg gcaagaacca gatcgacagg tactacgaaa
     4921 cttgtatcgg aaaggataag ggcaagagcg tgagcgaaaa ggtggacgct ctcacaaaga
     4981 tcatcaccgg aatgaactac gaccaattcg acaagaaaag gagcgtcatt gaggacaccg
     5041 gcagggaaaa cgccgagagg gagaagttta aaaagatcat cagcctgtac ctcaccgtga
     5101 tctaccacat cctcaagaat attgtcaata tcaacgccag gtacgtcatc ggattccatt
     5161 gcgtcgagcg tgatgctcaa ctgtacaagg agaaaggcta cgacatcaat ctcaagaaac
     5221 tggaagagaa gggattcagc tccgtcacca agctctgcgc tggcattgat gaaactgccc
     5281 ccgataagag aaaggacgtg gaaaaggaga tggctgaaag agccaaggag agcattgaca
     5341 gcctcgagag cgccaacccc aagctgtatg ccaattacat caaatacagc gacgagaaga
     5401 aagccgagga gttcaccagg cagattaaca gggagaaggc caaaaccgcc ctgaacgcct
     5461 acctgaggaa caccaagtgg aatgtgatca tcagggagga cctcctgaga attgacaaca
     5521 agacatgtac cctgttcaga aacaaggccg tccacctgga agtggccagg tatgtccacg
     5581 cctatatcaa cgacattgcc gaggtcaatt cctacttcca actgtaccat tacatcatgc
     5641 agagaattat catgaatgag aggtacgaga aaagcagcgg aaaggtgtcc gagtacttcg
     5701 acgctgtgaa tgacgagaag aagtacaacg ataggctcct gaaactgctg tgtgtgcctt
     5761 tcggctactg tatccccagg tttaagaacc tgagcatcga ggccctgttc gataggaacg
     5821 aggccgccaa gttcgacaag gagaaaaaga aggtgtccgg caattccatc gatgactaca
     5881 aggacgacga tgacaagggt accgagggca ggggaagtct tctaacatgc ggggacgtgg
     5941 aggaaaatcc cgggcccacc ggtatgtcca aaggagaaga actgtttact ggtgttgtgc
     6001 caattttggt tgaactcgat ggtgatgtca acggacataa gttctcagtg agaggcgaag
     6061 gagaaggtga cgccaccatt ggaaaattga ctcttaaatt catctgtact actggtaaac
     6121 ttcctgtacc atggccgact ctcgtaacaa cgcttacgta cggagttcag tgcttttcga
     6181 gatacccaga ccatatgaaa agacatgact tttttaagtc ggctatgcct gaaggttacg
     6241 tgcaagaaag aacaatttcg ttcaaagatg atggaaaata taaaactaga gcagttgtta
     6301 aatttgaagg agatactttg gttaaccgca ttgaactgaa aggaacagat tttaaagaag
     6361 atggtaatat tcttggacac aaactcgaat acaattttaa tagtcataac gtatacatca
     6421 ctgctgataa gcaaaagaac ggaattaaag cgaatttcac agtacgccat aatgtagaag
     6481 atggcagtgt tcaacttgcc gaccattacc aacaaaacac ccctattgga gacggtccgg
     6541 tacttcttcc tgataatcac tacctctcaa cacaaacagt cctgagcaaa gatccaaatg
     6601 aaaaaggaac aggtggcggc ggaagttagg cggccgcaat tcactcctca ggtgcaggct
     6661 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     6721 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     6781 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     6841 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     6901 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     6961 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     7021 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     7081 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     7141 gctgtccctc ttctcttatg gagatccctc gacctgcacc gtcgaccagc tggtcgacgg
     7201 tgcaccgtcg accagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7261 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7321 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7381 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     7441 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7501 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7561 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     7621 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7681 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     7741 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaa
//
