LOCUS       sequence-45148-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pcDNA3.2 GW delCMV
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(182..286)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(361..380)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     611..630
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        611..629
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    659..783
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
       61 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
      121 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      301 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
      361 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
      421 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      481 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      541 atatacgcgt tgacattgat tattgactct ggctaactag agaacccact gcttactggc
      601 ttatcgaaat taatacgact cactataggg agacccaagc tggctagtta agctatcaac
      661 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      721 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      781 atgaatcaac tacttagatg gtattagtga cctgtagtcg accgacag
//
