LOCUS       sequence-451480-        8612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous expression plasmid, contains CAG promoter (CMV immediate 
            early enhancer and chicken beta actin promoter), Cas13d, and Citrine
            reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Kim M, Hutchins EJ
  TITLE     CRISPR-Cas13d as a molecular tool to achieve targeted gene 
            expression knockdown in chick embryos.
  JOURNAL   Dev Biol. 2024 Nov 30:S0012-1606(24)00265-3. doi: 
            10.1016/j.ydbio.2024.11.013.
  PUBMED    39622311
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2024 Nov 30:S0012-1606(24)00265-3. doi: 
            10.1016/j.ydbio.2024.11.013."
COMMENT     Sequence Label: pCAG-Cas13d-T2A-Citrine
FEATURES             Location/Qualifiers
     source          1..8612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1167..1546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1808..2824
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2748..2770
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2832..2851
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2959..5859
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="MIEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDS
                     IRSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLE
                     KRYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTV
                     YTYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYII
                     NYGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITN
                     ELTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRK
                     DMSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFV
                     INLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPA
                     GRDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAF
                     FKDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDE
                     NGNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQ
                     KKQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGRE
                     NAEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLE
                     EKGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKK
                     AEEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVH
                     AYINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCV
                     PFGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5866..5889
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5896..5949
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5956..5958,5959..5961,5962..6672)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6001..6022)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6262..6281)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6609..6630
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6766..6785)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6831..6886
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6885..6904)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7277..7293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7277..7293)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7290..7312)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7301..7317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7325..7331,7332..7349,7350..7355))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7370..7391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7450..7645
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7496..7631
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7651..7785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7688..7707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7742..7761
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7853..7870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8024..8612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8104..8123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc
     1201 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1261 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1321 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1381 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1441 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1501 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1561 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     1621 tattttttaa ttattttgtg cagcgatggg ggcggggggg ggggggggcg cgcgccaggc
     1681 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     1741 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     1801 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     1861 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     1921 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     1981 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     2041 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     2101 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     2161 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     2221 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     2281 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     2341 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     2401 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     2461 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     2521 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     2581 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     2641 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     2701 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     2761 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     2821 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct
     2881 cgagagttcg aagccttaag tacgcgtagg cgcgccttaa ttaacgttta aacatgcatg
     2941 caaatggcta gcgccaccat gatcgagaag aagaagtcct tcgccaaggg catgggcgtg
     3001 aagtccacac tcgtgtccgg ctccaaagtg tacatgacaa ccttcgccga aggcagcgac
     3061 gccaggctgg aaaagatcgt ggagggcgac agcatcagga gcgtgaatga gggcgaggcc
     3121 ttcagcgctg aaatggccga taaaaacgcc ggctataaga tcggcaacgc caaattcagc
     3181 catcctaagg gctacgccgt ggtggctaac aaccctctgt atacaggacc cgtccagcag
     3241 gatatgctcg gcctgaagga aactctggaa aagaggtact tcggcgagag cgctgatggc
     3301 aatgacaata tttgtatcca ggtgatccat aacatcctgg acattgaaaa aatcctcgcc
     3361 gaatacatta ccaacgccgc ctacgccgtc aacaatatct ccggcctgga taaggacatt
     3421 attggattcg gcaagttctc cacagtgtat acctacgacg aattcaaaga ccccgagcac
     3481 catagggccg ctttcaacaa taacgataag ctcatcaacg ccatcaaggc ccagtatgac
     3541 gagttcgaca acttcctcga taaccccaga ctcggctatt tcggccaggc ctttttcagc
     3601 aaggagggca gaaattacat catcaattac ggcaacgaat gctatgacat tctggccctc
     3661 ctgagcggac tgaggcactg ggtggtccat aacaacgaag aagagtccag gatctccagg
     3721 acctggctct acaacctcga taagaacctc gacaacgaat acatctccac cctcaactac
     3781 ctctacgaca ggatcaccaa tgagctgacc aactccttct ccaagaactc cgccgccaac
     3841 gtgaactata ttgccgaaac tctgggaatc aaccctgccg aattcgccga acaatatttc
     3901 agattcagca ttatgaaaga gcagaaaaac ctcggattca atatcaccaa gctcagggaa
     3961 gtgatgctgg acaggaagga tatgtccgag atcaggaaaa atcataaggt gttcgactcc
     4021 atcaggacca aggtctacac catgatggac tttgtgattt ataggtatta catcgaagag
     4081 gatgccaagg tggctgccgc caataagtcc ctccccgata atgagaagtc cctgagcgag
     4141 aaggatatct ttgtgattaa cctgaggggc tccttcaacg acgaccagaa ggatgccctc
     4201 tactacgatg aagctaatag aatttggaga aagctcgaaa atatcatgca caacatcaag
     4261 gaatttaggg gaaacaagac aagagagtat aagaagaagg acgcccctag actgcccaga
     4321 atcctgcccg ctggccgtga tgtttccgcc ttcagcaaac tcatgtatgc cctgaccatg
     4381 ttcctggatg gcaaggagat caacgacctc ctgaccaccc tgattaataa attcgataac
     4441 atccagagct tcctgaaggt gatgcctctc atcggagtca acgctaagtt cgtggaggaa
     4501 tacgcctttt tcaaagactc cgccaagatc gccgatgagc tgaggctgat caagtccttc
     4561 gctagaatgg gagaacctat tgccgatgcc aggagggcca tgtatatcga cgccatccgt
     4621 attttaggaa ccaacctgtc ctatgatgag ctcaaggccc tcgccgacac cttttccctg
     4681 gacgagaacg gaaacaagct caagaaaggc aagcacggca tgagaaattt cattattaat
     4741 aacgtgatca gcaataaaag gttccactac ctgatcagat acggtgatcc tgcccacctc
     4801 catgagatcg ccaaaaacga ggccgtggtg aagttcgtgc tcggcaggat cgctgacatc
     4861 cagaaaaaac agggccagaa cggcaagaac cagatcgaca ggtactacga aacttgtatc
     4921 ggaaaggata agggcaagag cgtgagcgaa aaggtggacg ctctcacaaa gatcatcacc
     4981 ggaatgaact acgaccaatt cgacaagaaa aggagcgtca ttgaggacac cggcagggaa
     5041 aacgccgaga gggagaagtt taaaaagatc atcagcctgt acctcaccgt gatctaccac
     5101 atcctcaaga atattgtcaa tatcaacgcc aggtacgtca tcggattcca ttgcgtcgag
     5161 cgtgatgctc aactgtacaa ggagaaaggc tacgacatca atctcaagaa actggaagag
     5221 aagggattca gctccgtcac caagctctgc gctggcattg atgaaactgc ccccgataag
     5281 agaaaggacg tggaaaagga gatggctgaa agagccaagg agagcattga cagcctcgag
     5341 agcgccaacc ccaagctgta tgccaattac atcaaataca gcgacgagaa gaaagccgag
     5401 gagttcacca ggcagattaa cagggagaag gccaaaaccg ccctgaacgc ctacctgagg
     5461 aacaccaagt ggaatgtgat catcagggag gacctcctga gaattgacaa caagacatgt
     5521 accctgttca gaaacaaggc cgtccacctg gaagtggcca ggtatgtcca cgcctatatc
     5581 aacgacattg ccgaggtcaa ttcctacttc caactgtacc attacatcat gcagagaatt
     5641 atcatgaatg agaggtacga gaaaagcagc ggaaaggtgt ccgagtactt cgacgctgtg
     5701 aatgacgaga agaagtacaa cgataggctc ctgaaactgc tgtgtgtgcc tttcggctac
     5761 tgtatcccca ggtttaagaa cctgagcatc gaggccctgt tcgataggaa cgaggccgcc
     5821 aagttcgaca aggagaaaaa gaaggtgtcc ggcaattcca tcgatgacta caaggacgac
     5881 gatgacaagg gtaccgaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat
     5941 cccgggccca ccggtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     6001 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     6061 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     6121 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgatgtgctt cgcccgctac
     6181 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     6241 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     6301 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     6361 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     6421 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     6481 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     6541 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     6601 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     6661 gagctgtaca agtgagcggc cgcaattcac tcctcaggtg caggctgcct atcagaaggt
     6721 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     6781 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     6841 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     6901 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     6961 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     7021 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     7081 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     7141 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     7201 cttatggaga tccctcgacc tgcaccgtcg accagctggt cgacggtgca ccgtcgacca
     7261 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7321 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7381 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7441 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7501 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7561 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7621 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     7681 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7741 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     7801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7861 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7921 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7981 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8041 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8101 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8161 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8221 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8281 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8341 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8401 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8461 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8521 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8581 agcagattac gcgcagaaaa aaaggatctc aa
//
