LOCUS       sequence-45149-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pcDNA3.2 GW delCMV
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    40..88
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     113..132
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(172..213)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    239..363
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        541..643
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(710..729)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg
       61 tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc
      121 ttccgtgttt cagttagcct cccccgttta aactcattac taaccggtac gcgtagaatc
      181 gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct ctagatcaac
      241 cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      301 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      361 atgaatcaac tacttagatg gtattagtga cctgtagtcg actaagttgg cagcatcacc
      421 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      481 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      541 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      601 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
      661 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
      721 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
      781 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
      841 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatgg
//
