LOCUS       sequence-451525-       17766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP
            seed selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17766)
  TITLE     CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP
            seed selection near RB
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEC1.2/EC1.1_Cas9i_TagRFP
FEATURES             Location/Qualifiers
     source          1..17766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             808..1503
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(1502..1753)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             2462..3253
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        3405..3480
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5063..5083
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10816..10836
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    11487..11511
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11936..12730
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12817..13405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13306..13325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13559..13576
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13591..13731
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13657..13676)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13817..13839
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14075..14269
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14335..15408)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15837..16466)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag ctgaaaccaa attaacatag ggtttttatt taaataaaag tttaaccttc
      301 tttttaaaaa attgttcata gtgtcatgtc agaacaagag ctacaaatca cacatagcat
      361 gcataagcgg agctatgatg agtggtattg ttttgttcgt cacttgtcac tcttttccaa
      421 cacataatcc cgacaacaac gtaagagcat ctctctctct ccacacacac tcatgcatgc
      481 atgcattctt acacgtgatt gccatgcaaa tctcctttct cacctataaa tacaaaccaa
      541 cccttcacta cactcttcac tcaaaccaaa acaagaaaac atacacaaat agcaaaacaa
      601 gggcgaattc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc aagcattcta
      661 cttctattgc agcaatttaa atcatttctt ttaaagcaaa agctattttc tgaaaatttt
      721 caccatttac gaacgatagc catggtgaag actaatcttt ttctcttttt catcttttca
      781 cttctcctat cattatcctc ggccgacatg agcgagctga ttaaggagaa catgcacatg
      841 aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa
      901 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
      961 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac cttcatcaac
     1021 cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag
     1081 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     1141 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct
     1201 gtgatgcaga agaaaacact cggctgggag gccaacaccg agatgctgta ccccgctgac
     1261 ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc
     1321 tgcaacttca agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     1381 gtctactatg tggaccacag actggaaaga atcaaggagg ccgacaaaga aacctacgtc
     1441 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaag
     1501 tgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     1561 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     1621 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     1681 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     1741 atgtttgaac gatagcttcg acttgccttc cgcacaatac atcatttctt cttagctttt
     1801 tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg
     1861 tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt
     1921 ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat
     1981 cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga
     2041 agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg
     2101 aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac
     2161 agctagagtc gaagtagtga ttgggagacc aacccagtgg acataagcct gttcggttcg
     2221 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     2281 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     2341 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     2401 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     2461 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2521 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2581 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2641 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2701 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2761 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2821 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2881 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2941 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     3001 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     3061 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     3121 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3181 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     3241 agtcggcaaa taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc
     3301 ttaactcaag cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa
     3361 gtctgctttt attattttta agcgtgcata ataagccggt ctcggtttta gagctagaaa
     3421 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     3481 ttttttttgc aaaattttcc agatcgattt cttcttcctc tgttcttcgg cgttcaattt
     3541 ctggggtttt ctcttcgttt tctgtaactg aaacctaaaa tttgacctaa aaaaaatctc
     3601 aaataatatg attcagtggt tttgtacttt tcagttagtt gagttttgca gttccgatga
     3661 gataaaccaa taccatggtt atactagtga ataaaagcat ttgcgtttgg tttatcattg
     3721 cgtttataca aggacagaga tccactgagc tggaatagct taaaaccatt atcagaacaa
     3781 aataaaccat tttttgttaa gaatcagagc atagtaaaca acagaaacaa cctaagagag
     3841 gtaacttgtc caagaagata gctaattata tctattttat aaaagttatc atagtttgta
     3901 agtcacaaaa gatgcaaata acagagaaac taggagactt gagaatatac attcttgtat
     3961 atttgtattc gagattgtga aaatttgacc ataagtttaa attcttaaaa agatatatct
     4021 gatctaggtg atggttatag actgtaattt taccacatgt ttaatgatgg atagtgacac
     4081 acatgacaca tcgacaacac tatagcatct tatttagatt acaacatgaa atttttctgt
     4141 aatacatgtc tttgtacata atttaaaagt aattcctaag aaatatattt atacaaggag
     4201 tttaaagaaa acatagcata aagttcaatg agtagtaaaa accatataca gtatatagca
     4261 taaagttcaa tgagtttatt acaaaagcat tggttcactt tctgtaacac gacgttaaac
     4321 cttcgtctcc aataggagcg ctactgattc aacatgccaa tatatactaa atacgtttct
     4381 acagtcaaat gctttaacgt ttcatgatta agtgactatt taccgtcaat cctttcccat
     4441 tcctcccact aatccaactt tttaattact cttaaatcac cactaagcta gtaacgccta
     4501 tcatgaatta gctctactaa atctagcaac ctttcaaatt tgcagtattg caggtgtctc
     4561 tgtgtcttta aaatagttgc cttatgattt cttcggtttc aagatgatca aatagttata
     4621 gatttcatgc tcacacatgc tcattagatg tgtacatact ttacttaccc aaatctattt
     4681 tctcgcaaag attttgatgg taaagctgat ttggttctat tgaactaaat caaacgagtt
     4741 tcagactgag tgattctaat ccggcccatt agcccctaaa cagacccact aattacgcag
     4801 cttttaatag agtaattaca cctagtttac ccactaaacc actaagcact aattatctca
     4861 caatctaatg agcttccctc gtaattactt gggctttcac tctaccattt atttgtaaca
     4921 gtcaagtctc tactgtctct atataaactc tctaaagtta acacacaatt ctcatcacaa
     4981 acaaatcaac caaagcaact tctactcttt cttctttcga ccttatcaat ctgttgagaa
     5041 atctagaatg gcttctagcc caccgaagaa gaagcggaag gtcagctgga aaatggacaa
     5101 gaagtacagc attggacttg atattggtac gaactcagtt gggtgggccg ttatcaccga
     5161 tgaatacaag gtaccttcga agaaatttaa agtgctgggc aacacagata ggcacagcat
     5221 taagaagaac ttgatcggag ctctgctctt tgactctgga gaaaccgcgg aggcgacaag
     5281 gcttaaacgt actgcgagga gaaggtacac tcgcaggaag aacagaatct gttatctcca
     5341 agagatcttt agcaacgaga tggcgaaggt aaggattttt atgatatagt atgcttatgt
     5401 attttgtact gaaagcatat cctgcttcat tgggatatta ctgaaagcat ttaactacat
     5461 gtaaactcac ttgatgatca ataaacttga ttttgcaggt tgacgactcg ttcttccatc
     5521 gcctcgagga atccttcctg gtagaggaag ataagaaaca cgagcgtcac cccatctttg
     5581 ggaatattgt tgacgaagta gcctatcatg aaaagtatcc gactatatac caccttcgca
     5641 agaagctggt ggactcaacc gataaggcag accttcggct catatacctg gctctcgcgc
     5701 acatgataaa gtttcgtggc catttcttga tcgaagggga cctcaacccg gataactccg
     5761 atgtggataa actgttcatt cagctcgtcc aaacctacaa tcagctgttc gaggagaacc
     5821 ccatcaatgc atcaggtaac attccttagt tacctttctt ttctttttcc atcataagtt
     5881 tatagattgt acatgctttg agatttttct ttgcaaacaa tctcaggtgt cgacgccaag
     5941 gcaatactgt ctgccagact ttcgaagtcc agacggcttg agaatctgat cgctcaattg
     6001 ccaggcgaga agaagaacgg cttgttcggg aatctgattg cactgtctct gggcctcacc
     6061 cctaacttca aaagcaactt tgacctcgcc gaggacgcga agctgcagct gtcaaaggat
     6121 acatacgatg atgatctgga caatctgctc gcccaaatag gtgctcttga aattggaact
     6181 cttcttttgt tgtctaaacc tatcaatttc tttgcggaaa tttatttgaa gctgtagagt
     6241 taaaattgag tcttttaaac ttttgtaggt gatcagtatg ccgacctgtt cttggctgcc
     6301 aagaatctgt cagacgctat cttgctcagt gacattctgc gggtcaacac ggagataacc
     6361 aaagcgccac ttagcgcctc catgatcaag aggtacgacg agcatcacca ggatctgacc
     6421 cttctgaagg ctttggttcg ccagcaactc cccgagaagt acaaggagat tttctttgac
     6481 caatcgaaga atggctacgc agggtacatt gatggaggta agttgttact tatgattgtt
     6541 ttcctctctg ctacatgtat tttgttgttc atttctgtaa gatataagaa ttgagttttc
     6601 ctctgatgat attattaggt gcaagtcagg aggaattcta caaattcatc aagcctattc
     6661 tggaaaagat ggacggtaca gaggagctgc tcgttaaatt gaaccgcgaa gatttgcttc
     6721 ggaagcagcg taccttcgac aatggcagca taccgcacca gatccacctc ggtgagctgc
     6781 atgctatctt gaggaggcaa gaggacttct atccgttcct gaaagacaac agagagaaga
     6841 ttgaaaagat cctcacgttc cgcattccct actatgtagg ttagtatcat atgaagaaat
     6901 acctagtttc agttgatgaa tgctattttc tgacctcagt tgttctcttt tgagaattat
     6961 ttcttttcta atttgcctga tttttctatt aattcattag gtccactcgc acgcgggaac
     7021 tcgcggtttg cgtggatgac acgcaaatcc gaggagacta tcacgccttg gaacttcgaa
     7081 gaggtcgtgg acaagggtgc gagtgcacag tccttcatcg aaaggatgac caacttcgat
     7141 aagaatctcc caaatgagaa agtcctgccc aagcatagtc tcctgtacga atacttcacg
     7201 gtctacaacg agctgacgaa ggtgaaatat gtgacggagg ggatgcgcaa accggccttc
     7261 ctgtcaggta aatcctggtc cacactttta cgataaaaac acaagatttt aaactatgaa
     7321 ctgatcaata atcattccta aaagaccaca cttttgtttt gtttctaaag taatttttac
     7381 tgttataaca ggtgagcaga agaaggccat tgtcgatctc ttgttcaaaa ccaatcggaa
     7441 ggtcactgtg aaacagctta aagaggacta ctttaagaag atcgaatgct ttgattctgt
     7501 ggaaatcagc ggcgttgagg ataggttcaa tgcctctctt ggcacatacc atgacctgtt
     7561 gaaaatcatc aaggacaagg acttccttga caacgaggag aacgaggaca tcctcgagga
     7621 catcgtgctg actctcacgc tgtttgagga cagagaaatg atcgaggagc gccttaagac
     7681 ttatgcgcat ctgttcgatg acaaggtcat gaagcagttg aagaggagga gatatacagg
     7741 taagaggtca aaaggtttcc gcaatgatcc ctcttttttt gtttctctag tttcaagaat
     7801 ttgggtatat gactaacttc tgagtgttcc ttgatgcata tttgtgatga gacaaatgtt
     7861 tgttctatgt tttaggttgg ggaaggctct ccaggaagct catcaacggc atccgcgaca
     7921 agcaatccgg caagactata ctggactttc tcaaatccga cggttttgcg aatcggaact
     7981 tcatgcagct tattcacgat gactcactga ccttcaaaga agatatccag aaggcccaag
     8041 tgtcaggtca gggcgatagc cttcacgaac acatagccaa cctggctgga tcgccagcta
     8101 taaagaaggg catactgcag acagtgaagg ttgtggatga gctggtgaag gtaagttctg
     8161 catttggtta tgctccttgc attttaggtg ttcgtcgcac ttccatttcc atgaatagct
     8221 aagatttttt ttctctgcat tcattcttct tgcctcagtt ctaactgttt gtggtatttt
     8281 tgttttaatt attgctacag gtcatgggcc gccataagcc ggagaacatc gtcatcgaga
     8341 tggcgaggga aaaccagacg actcagaaag ggcagaagaa ctcacgggag cgcatgaagc
     8401 ggatagagga aggcatcaag gagcttggga gtcagattct gaaagagcac ccagtcgaaa
     8461 atactcaact ccagaacgag aagctgtacc tctattacct ccagaatggg agagatatgt
     8521 acgtcgacca agagctcgac attaacagac tctccgacta tgatgtggat cacattgtcc
     8581 ctcaatcttt cctgaaggac gatagtattg acaacaaggt aaagcaactg tgttttaatc
     8641 aatttcttgt caggatatat ggattataac ttaatttttg agaaatctgt agtatttggc
     8701 gtgaaatgag tttgcttttt ggtttctccc gtgttatagg tccttacgcg ctcagacaag
     8761 aaccgcggaa aatccgacaa tgtacccagc gaggaggttg tgaagaagat gaagaactat
     8821 tggaggcagc ttttgaatgc taagctcata acccaacgga aattcgacaa tctcacgaag
     8881 gcagaaaggg gcggactgtc tgagctcgac aaagccggct tcatcaagcg ccagttggtt
     8941 gaaactcgtc agattacgaa acatgtggcc cagatactcg attcgcgtat gaatacgaag
     9001 tatgatgaga atgacaaact tatcagggag gtaaaggtaa agtttccaac tttcctttac
     9061 catatcaaac taaagttcga aactttttat ttgatcaact tcaaggccac ccgatctttc
     9121 tattcctgat taatttgtga tgaatccata ttgacttttg atggttacgc aggtgatcac
     9181 cctcaagagc aaactggtta gtgacttccg gaaggacttc cagttttaca aggttcgcga
     9241 gatcaacaac taccatcatg cccatgacgc ctacctgaac gccgttgttg gcactgctct
     9301 catcaagaag tatccgaaac tggagtctga gtttgtgtac ggggattaca aggtgtacga
     9361 cgttaggaag atgatcgcga agtcagaaca agagatcggc aaggctaccg cgaaatactt
     9421 cttttactcg aatatcatga acttcttcaa gacagagatc actctggcga atggtgaaat
     9481 ccggaagagg cctctgatcg agacaaatgg cgaaacaggt ctgtctttcc tatttcatat
     9541 gtttaatcct aggaatttga tcaattgatt gtatgtatgt cgatcccaag actttcttgt
     9601 tcacttatat cttaactctc tctttgctgt ttcttgcagg tgagattgtc tgggataagg
     9661 gcagggattt tgcgactgtg cgtaaggttc tcagcatgcc ccaagtcaac atagtcaaga
     9721 aaacggaggt tcaaaccggt ggtttctcca aggagtccat tctccctaag cgcaactccg
     9781 acaaactgat tgcgaggaag aaggattggg atccgaagaa atacggaggc tttgatagcc
     9841 ctaccgtggc atacagcgta ctggtagtgg ccaaggtgga gaagggcaag agcaagaaac
     9901 tgaaaagcgt caaggaactg cttggaatta ccataatgga aaggtcctcg ttcgagaaga
     9961 atccgatcga cttcctcgag gctaaaggta aaatattgga tgccagacga tattctttct
    10021 tttgatttgt aactttttcc tgtcaaggtc gataaatttt attttttttg gtaaaaggtc
    10081 gataattttt ttttggagcc attatgtaat tttcctaatt aactgaacca aaattataca
    10141 aaccaggtta caaagaggtg aagaaagacc tcattatcaa actgcccaag tattcgcttt
    10201 tcgaattgga aaatggcaga aaacgcatgc tggcatctgc cggagaactg cagaagggca
    10261 acgagctggc attgcccagt aagtacgtca acttcctgta cttggcctca cactatgaga
    10321 agctgaaggg gtcaccagag gacaacgagc agaagcagtt gtttgtcgag cagcacaagc
    10381 actatcttga tgagatcata gagcagatca gcgaattttc caagcgggtc attcttgcag
    10441 acgctaacct cgataaggta aggacttctc atgaatatta gtggcagatt agtgttgtta
    10501 aagtctttgg ttagataatc gatgcctcct aattgtccat gttttactgg ttttctacaa
    10561 ttaaaggtgc tttccgcgta caacaagcac agagataagc cgataaggga acaagcggaa
    10621 aacatcatcc acctgttcac actgaccaat ctgggagccc cagcagcctt taagtacttc
    10681 gataccacta tcgacagaaa gcgctacaca tcaaccaagg aagtgttgga cgctaccctt
    10741 attcaccaat ctattacagg gctctatgag acaaggatag atctgtcgca gttgggtggt
    10801 gactctaggg ctgacccaaa gaagaagcgt aaagtctgag agctcagagc tttcgttcgt
    10861 atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc gcacacacca
    10921 gaatcctact gagtttgagt attatggcat tgggaaaact gtttttcttg taccatttgt
    10981 tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa
    11041 tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat
    11101 ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt
    11161 ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag
    11221 taaaacactt gtagttgtac cattatgctt attcactagg caacaaatat attttcagac
    11281 ctagaaaagc tgcaaatgtt actgaataca agtatgtcct cttgtgtttt agacatttat
    11341 gaactttcct ttatgtaatt ttccagaatc cttgtcagat tctaatcatt gctttataat
    11401 tatagttata ctcatggatt tgtagttgag tatgaaaata ttttttaatg cattttatga
    11461 cttgccaatt gattgacaac ggtaccgttt acaccacaat atatcctgcc accagccagc
    11521 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
    11581 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
    11641 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
    11701 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca
    11761 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
    11821 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
    11881 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
    11941 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
    12001 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
    12061 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
    12121 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
    12181 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
    12241 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
    12301 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
    12361 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
    12421 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
    12481 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
    12541 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
    12601 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
    12661 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
    12721 attgttttag tacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    12781 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    12841 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    12901 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    12961 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    13021 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    13081 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    13141 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    13201 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    13261 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    13321 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    13381 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    13441 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    13501 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    13561 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
    13621 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
    13681 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
    13741 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    13801 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    13861 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
    13921 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
    13981 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
    14041 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
    14101 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
    14161 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
    14221 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaacagacc
    14281 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
    14341 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
    14401 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
    14461 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
    14521 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
    14581 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
    14641 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
    14701 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
    14761 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
    14821 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
    14881 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
    14941 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
    15001 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
    15061 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
    15121 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
    15181 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
    15241 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
    15301 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
    15361 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
    15421 ggcttggggg ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc
    15481 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
    15541 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
    15601 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
    15661 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
    15721 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
    15781 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
    15841 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
    15901 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
    15961 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
    16021 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
    16081 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
    16141 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
    16201 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    16261 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    16321 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    16381 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    16441 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    16501 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    16561 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    16621 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    16681 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    16741 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    16801 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    16861 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    16921 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    16981 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    17041 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    17101 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    17161 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    17221 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    17281 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    17341 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    17401 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    17461 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    17521 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    17581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    17641 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    17701 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    17761 tcttag
//
