LOCUS       sequence-451528-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible expression of RquA-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Valeros J, Jerome M, Tseyang T, Vo P, Do T, Fajardo Palomino D, 
            Grotehans N, Kunala M, Jerrett AE, Hathiramani NR, Mireku M, Magesh 
            RY, Yenilmez B, Rosen PC, Mann JL, Myers JW, Kunchok T, Manning TL, 
            Boercker LN, Carr PE, Munim MB, Lewis CA, Sabatini DM, Kelly M, Xie 
            J, Czech MP, Gao G, Shepherd JN, Walker AK, Kim H, Watson EV, 
            Spinelli JB
  TITLE     Rhodoquinone carries electrons in the mammalian electron transport 
            chain.
  JOURNAL   Cell. 2025 Jan 10:S0092-8674(24)01420-X. doi: 
            10.1016/j.cell.2024.12.007.
  PUBMED    39909039
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Jan 10:S0092-8674(24)01420-X. doi: 10.1016/j.cell.2024.12.007."
COMMENT     Sequence Label: pcw57.1-RquA-FLAG
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             790..813
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgggtatgc ttcggttacc cacagtcttt cgccagatga gaccggtgtc cagggtactg
       61 gctcctcatc tcactcgggc ttatgccaaa atgagcggca atcccccgga gtttcggcct
      121 gcaccgtact atgatggggt ccctgactat atggttgatg tctatgattg ggcgtacgtc
      181 aaccctgcca aagctcggct gttggatcgc aatattgttg ttagcactct gcttttcctg
      241 aatgatcagc gactgatgcg gcgctatctc gccaggatta gaccgggctc aagagtctgg
      301 caggttgcac acgtctatgg tgacctggtg tctcgagctg cccaagcggt aggcacggat
      361 ggtaggtttg tcttgacaga tattactccc gtgcaggtcg agcatgcgct taaaaaactg
      421 gcgccttacc cacaagccga agttgtcaga gccgatgcca gttcctggca agctgatgcg
      481 cccttcgacc tggtgtgctc tttcttcctt ctgcacgaag taccagacga actcaaggca
      541 gcaatcgtcg ataatatgct tgctcaagtc gcccctggcg gggagttggt gttcgttgac
      601 tatcacagac ctcgaccctg gcagcccata ggctggctgc tgaagtatgt gaatagggtt
      661 ctcgagcctt tcgcagaagc gctttggaac aaggatatag cggattacgc cacgcgcaaa
      721 gacgatttcg tgtggcaaaa ggagaccgtg ttcggaggtg tataccaaat cgtgcgagtc
      781 agagcccggg attacaagga tgacgatgac aagtag
//
