LOCUS       sequence-45154-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of Ef1a-driven, cre-dependent, stabilized step 
            function opsin (SSFO) for optogenetic activation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, 
            Sohal VS, Goshen I, Finkelstein J, Paz JT, Stehfest K, Fudim R, 
            Ramakrishnan C, Huguenard JR, Hegemann P, Deisseroth K
  TITLE     Neocortical excitation/inhibition balance in information processing 
            and social dysfunction.
  JOURNAL   Nature. 2011 Jul 27;477(7363):171-8. doi: 10.1038/nature10360.
  PUBMED    21796121
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-07-27- 27"; volume: "477"; issue: "7363"; 
            pages: "171-8"
COMMENT     Sequence Label: pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcggccgct ggcacggctc cggcctcggc ttcgtcttcg
       61 acgagagtct cgacctcgat ctccgttccg ccgatgttca gtttggtggt tttgcggata
      121 tctccgtgaa tcaatatgtg ctcgtggatc aggacgcgca ggtagtgtcc caacaacccc
      181 caacaatttt tactcatcag atcaataatc gtgtgaccta cggtggagcc atagacgctc
      241 aggacgccaa aaccttcggg ccccaaaatg aagagaattg ggaacatacc ccagctcacg
      301 aaaaacagcc atgccatgcc ggtcacgacc tggcggcacc gaccctttgg cacagtatga
      361 taaccctcga tatatgcttt ggcggcgtga aaaaatgtgt tcgcgccata gcacaatcca
      421 agacaaaaga agatgacttt aacatagccg gttgccatgg cgctggtagc cccccacacg
      481 atagtcccga tggctgagac aaggagtccc atggttctcc tgctgtagtc gttgctcagg
      541 ccggtgaggt tgctcaggtg gataaggatg acaggagaag tgagcagcca ctctgcatag
      601 cgcagccact gcacccggtg tcctgtggca aggtagagca tagagggatt cttaa
//
