LOCUS       sequence-45155-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Sims JJ, Scavone F, Cooper EM, Kane LA, Youle RJ, Boeke JD, Cohen RE
  TITLE     Polyubiquitin-sensor proteins reveal localization and linkage-type 
            dependence of cellular ubiquitin signaling.
  JOURNAL   Nat Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888.
  PUBMED    22306808
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888."
COMMENT     Sequence Label: pQCXIP-Vx3K0-mEGFP
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(281..307)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(281..307)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      316..325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(446..470)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(467..566)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(496..516)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cctcgccctt gctcaccatg gtggcgaccg gtggatcccc agcagagtta cggctctcgc
       61 gcaggctcag ctcgatggcg cggcggatca gctcctcctc gtcctcttcc tgagcgttaa
      121 cttcacgaga ttcgcgcaga gacagttcga tagcgcgacg gatcagttct tcttcgtctt
      181 cgcgaacgcg ttcttcagca cgagcttcac gcagagattc ctggatagca gcgcgtaaat
      241 caggatcttc ttcttcttcc atctcgaggc cgctgccgcc ggcgtagtcg ggcacgtcgt
      301 aggggtagct gccctccatg gtggcggcgc ggccgcggat caattcctgc aggtccgttc
      361 caatgcaccg ttcccggccg cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa
      421 aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct ctgcttatat
      481 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      541 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
      601 ggagacttgg aaat
//
