LOCUS       sequence-451559-        9597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lambda immunity region to the luciferase operon (lux).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Silpe JE, Wong JWH, Owen SV, Baym M, Balskus EP
  TITLE     The bacterial toxin colibactin triggers prophage induction.
  JOURNAL   Nature. 2022 Mar;603(7900):315-320. doi: 10.1038/s41586-022-04444-3.
            Epub 2022 Feb 23.
  PUBMED    35197633
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04444-3"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7900"; pages: "315-320"
COMMENT     Sequence Label: pR_Lux
FEATURES             Location/Qualifiers
     source          1..9597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             684..1766
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             1781..2764
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             2943..4055
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      4088..4174
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     oriT            4735..4844
                     /note="incP origin of transfer"
     CDS             complement(5188..6003)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5188..6003)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5911..5930
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5995..6014
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6207..6224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6341..6886)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6421..6440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7040..7753)
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLARILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     RBS             7842..7850
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7854..9296
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             join(9309..9597,1..635)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
ORIGIN
        1 cagtggttga ttggttaact acacgaaaaa taaataactt cggtatgttg gcttcaagct
       61 tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg
      121 cagtcggtgt tgttaactta agatattctc ttgaaagagc tttagggttt gattatctca
      181 gtctacccat taatgaattg ccgaataatc tagattttga aggccataaa ttgggtgctg
      241 aagtctttgc gagagattgt cttgattttg gttgggaaga tttagcttct acaattaata
      301 acatgatgta tcttgatata ccgtttattg cttttactgc aaataacgat aattgggtca
      361 agcaagatga agttatcaca ttgttatcaa atattcgtag taatcgatgc aagatatatt
      421 ctttgttagg aagttcgcat gacttgagtg aaaatttagt ggtcctgcgc aatttttatc
      481 aatcggttac gaaagccgct atcgcgatgg ataatgatca tctggatatt gatgttgata
      541 ttactgaacc gtcatttgaa catttaacta ttgcgacagt caatgaacgc cgaatgagaa
      601 ttgagattga aaatcaagca atttctctgt cttaaaatct attgagatat tctatcactc
      661 aaatagcaat ataaggactc tctatgaaat ttggaaactt tttgcttaca taccaacctc
      721 cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg
      781 agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg
      841 gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa
      901 ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat
      961 tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg
     1021 actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg
     1081 ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa catatcaagt
     1141 tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg
     1201 tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa
     1261 gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc
     1321 aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata acatctgtag
     1381 atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt
     1441 cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt tatgatttca
     1501 ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat cgccgtattg
     1561 attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac ataattcaaa
     1621 aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct aatggaacag
     1681 tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca tttcttaaag
     1741 aaaaacaacg ttcgctatta tattagctaa ggagaaagaa atgaaatttg gattgttctt
     1801 ccttaacttc atcaattcaa caactgttca agaacaaagt atagttcgca tgcaggaaat
     1861 aacggagtat gttgataagt tgaattttga acagatttta gtgtatgaaa atcatttttc
     1921 agataatggt gttgtcggcg ctcctctgac tgtttctggt tttctgctcg gtttaacaga
     1981 gaaaattaaa attggttcat taaatcacat cattacaact catcatcctg tccgcatagc
     2041 ggaggaagct tgcttattgg atcagttaag tgaagggaga tttattttag ggtttagtga
     2101 ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg gttgaatatc aacagcaact
     2161 atttgaagag tgttatgaaa tcattaacga tgctttaaca acaggctatt gtaatccaga
     2221 taacgatttt tatagcttcc ctaaaatatc tgtaaatccc catgcttata cgccaggcgg
     2281 acctcggaaa tatgtaacag caaccagtca tcatattgtt gagtgggcgg ccaaaaaagg
     2341 tattcctctc atctttaagt gggatgattc taatgatgtt agatatgaat atgctgaaag
     2401 atataaagcc gttgcggata aatatgacgt tgacctatca gagatagacc atcagttaat
     2461 gatattagtt aactataacg aagatagtaa taaagctaaa caagagacgc gtgcatttat
     2521 tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc gaaaataaac ttgaagaaat
     2581 aattgcagaa aacgctgtcg gaaattatac ggagtgtata actgcggcta agttggcaat
     2641 tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa ccaatgaatg atttgatgag
     2701 ccaaaaaaat gtaatcaata ttgttgatga taatattaag aagtaccaca tggaatatac
     2761 ctaatagatt tcgagttgca gcgaggcggc aagtgaacga atccccagga gcatagataa
     2821 ctatgtgact ggggtgagtg aaagcagcca acaaagcagc agcttgaaag atgaagggta
     2881 taaaagagta tgacagcagt gctgccatac tttctaatat tatcttgagg agtaaaacag
     2941 gtatgacttc atatgttgat aaacaagaaa ttacagcaag ctcagaaatt gatgatttga
     3001 ttttttcgag cgatccatta gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac
     3061 ttgtgcttga tgcatttcgt aatcattata aacattgtcg agaatatcgt cactactgtc
     3121 aggcacacaa agtagatgac aatattacgg aaattgatga catacctgta ttcccaacat
     3181 cggtttttaa gtttactcgc ttattaactt ctcaggaaaa cgagattgaa agttggttta
     3241 ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg tgacagatta agtattgaga
     3301 gactcttagg ctctgtgagt tatggcatga aatatgttgg tagttggttt gatcatcaaa
     3361 tagaattagt caatttggga ccagatagat ttaatgctca taatatttgg tttaaatatg
     3421 ttatgagttt ggtggaattg ttatatccta cgacatttac cgtaacagaa gaacgaatag
     3481 attttgttaa aacattgaat agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc
     3541 ttattggttc gccatacttt atttatttac tctgccatta tatgaaagat aaaaaaattt
     3601 cattttctgg agataaaagc ctttatatca taaccggagg cggctggaaa agttacgaaa
     3661 aagaatctct gaaacgtgat gatttcaatc atcttttatt tgatactttc aatctcagtg
     3721 atattagtca gatccgagat atatttaatc aagttgaact caacacttgt ttctttgagg
     3781 atgaaatgca gcgtaaacat gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa
     3841 cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa
     3901 ccagttatcc agcatttatt gttaccgatg atgtcgggat aattagcaga gaatatggta
     3961 agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt
     4021 gtgctttaag cttaaccgaa gcgtttgata gttgatatcc tttgcctgcg gccgcaacta
     4081 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     4141 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagacct agggtacggg
     4201 ttttgctgcc cgcaaacggg ctgttctggt gttgctagtt tgttatcaga atcgcagatc
     4261 cggcttcagc cggtttgccg gctgaaagcg ctatttcttc cagaattgcc atgatttttg
     4321 gatccggtga ttgattgagc aagctttatg cttgtaaacc gttttgtgaa aaaattttta
     4381 aaataaaaaa ggggacctct agggtcccca attactagcc aattcgatta ctggcttact
     4441 atgttggcac tgatgagggt gtcagtgaag tgacctgcag gggggggggc gctgaggtct
     4501 gcctcgtgaa gaaggtgttg ctgactcata ccaggcttaa ttcctgcatt tgcctgtttc
     4561 cagtcggtag atattccaca aaacagcagg gaagcagcgc ttttccgctg cataaccctg
     4621 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     4681 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     4741 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     4801 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg ccggcgtaac
     4861 agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca gcccacctat
     4921 caaggtgtac tgccttccag aattaagcct gaatcgcccc atcatccagc cagaaagtga
     4981 gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt
     5041 gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag
     5101 caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca
     5161 gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     5221 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     5281 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     5341 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     5401 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat
     5461 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     5521 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     5581 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     5641 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg
     5701 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     5761 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     5821 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     5881 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     5941 agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct
     6001 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
     6061 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttccccccc
     6121 ccccctgcag gtccttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat
     6181 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     6241 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     6301 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     6361 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     6421 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     6481 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     6541 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     6601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     6661 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     6721 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     6781 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     6841 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     6901 taatcagata atatattttt agcggagtga ctataaaaag gcgcactgat caatgcgcct
     6961 tttttataag atcataaaag acccttcatt tatgaagggt ctttttgcat gcggcgggta
     7021 ccgctcgagt taattaagat cagccaaacg tctcttcagg ccactgacta gcgataactt
     7081 tccccacaac ggaacaactc tcattgcatg ggatcattgg gtactgtggg tttagtggtt
     7141 gtaaaaacac ctgaccgcta tccctgatca gtttcttgaa ggtaaactca tcacccccaa
     7201 gtctggctat gcagaaatca cctggctcaa cagcctgctc agggtcaacg agaattaaca
     7261 ttccgtcagg aaagcttggc ttggagcctg ttggtgcggt catggaatta ccttcaacct
     7321 caagccagaa tgcagaatca ctggcttttt tggttgtgct tacccatctc tccgcatcac
     7381 ctttggtaaa ggttctaagc tcaggtgaga acatccctgc ctgaacatga gaaaaaacag
     7441 ggtactcata ctcacttcta agtgacggct gcatactaac cgcttcatac atctcgtaga
     7501 tttctctggc gattgaaggg ctaaattctt caacgctaac tttgagaatt cttgcaagca
     7561 atgcggcgtt ataagcattt aatgcattga tgccattaaa taaagcacca acgcctgact
     7621 gccccatccc catcttgtct gcgacagatt cctgggataa gccaagttca tttttctttt
     7681 tttcataaat tgctttaagg cgacgtgcgt cctcaagctg ctcttgtgtt aatggtttct
     7741 tttttgtgct catacgttaa atctatcacc gcaagggata aatatctaac accgtgcgtg
     7801 ttgactattt tacctctggc ggtgataatg gttgcatgta ctaaggaggt tgtatgacta
     7861 aaaaaatttc attcattatt aacggccagg ttgaaatctt tcccgaaagt gatgatttag
     7921 tgcaatccat taattttggt gataatagtg tttacctgcc aatattgaat gactctcatg
     7981 taaaaaacat tattgattgt aatggaaata acgaattacg gttgcataac attgtcaatt
     8041 ttctctatac ggtagggcaa agatggaaaa atgaagaata ctcaagacgc aggacataca
     8101 ttcgtgactt aaaaaaatat atgggatatt cagaagaaat ggctaagcta gaggccaatt
     8161 ggatatctat gattttatgt tctaaaggcg gcctttatga tgttgtagaa aatgaacttg
     8221 gttctcgcca tatcatggat gaatggctac ctcaggatga aagttatgtt cgggcttttc
     8281 cgaaaggtaa atctgtacat ctgttggcag gtaatgttcc attatctggg atcatgtcta
     8341 tattacgcgc aattttaact aagaatcagt gtattataaa aacatcgtca accgatcctt
     8401 ttaccgctaa tgcattagcg ttaagtttta ttgatgtaga ccctaatcat ccgataacgc
     8461 gctctttatc tgttatatat tggccccacc aaggtgatac atcactcgca aaagaaatta
     8521 tgcgacatgc ggatgttatt gtcgcttggg gagggccaga tgcgattaat tgggcggtag
     8581 agcatgcgcc atcttatgct gatgtgatta aatttggttc taaaaagagt ctttgcatta
     8641 tcgataatcc tgttgatttg acgtccgcag cgacaggtgc ggctcatgat gtttgttttt
     8701 acgatcagcg agcttgtttt tctgcccaaa acatatatta catgggaaat cattatgagg
     8761 aatttaagtt agcgttgata gaaaaactta atctatatgc gcatatatta ccgaatgcca
     8821 aaaaagattt tgatgaaaag gcggcctatt ctttagttca aaaagaaagc ttgtttgctg
     8881 gattaaaagt agaggtggat attcatcaac gttggatgat tattgagtca aatgcaggtg
     8941 tggaatttaa tcaaccactt ggcagatgtg tgtaccttca tcacgtcgat aatattgagc
     9001 aaatattgcc ttatgttcaa aaaaataaga cgcaaaccat atctattttt ccttgggagt
     9061 catcatttaa atatcgagat gcgttagcat taaaaggtgc ggaaaggatt gtagaagcag
     9121 gaatgaataa catatttcga gttggtggat ctcatgacgg aatgagaccg ttgcaacgat
     9181 tagtgacata tatttctcat gaaaggccat ctaactatac ggctaaggat gttgcggttg
     9241 aaatagaaca gactcgattc ctggaagaag ataagttcct tgtatttgtc ccataatagg
     9301 taaaaagtat ggaaaatgaa tcaaaatata aaaccatcga ccacgttatt tgtgttgaag
     9361 gaaataaaaa aattcatgtt tgggaaacgc tgccagaaga aaacagccca aagagaaaga
     9421 atgccattat tattgcgtct ggttttgccc gcaggatgga tcattttgct ggtctggcgg
     9481 aatatttatc gcggaatgga tttcatgtga tccgctatga ttcgcttcac cacgttggat
     9541 tgagttcagg gacaattgat gaatttacaa tgtctatagg aaagcagagc ttgttag
//
