LOCUS       sequence-451565-        7596 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TAM Receptor Ligand human GAS6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Geng K, Kumar S, Kimani SG, Kholodovych V, Kasikara C, Mizuno K, 
            Sandiford O, Rameshwar P, Kotenko SV, Birge RB
  TITLE     Requirement of Gamma-Carboxyglutamic Acid Modification and 
            Phosphatidylserine Binding for the Activation of Tyro3, Axl, and 
            Mertk Receptors by Growth Arrest-Specific 6.
  JOURNAL   Front Immunol. 2017 Nov 10;8:1521. doi: 10.3389/fimmu.2017.01521. 
            eCollection 2017.
  PUBMED    29176978
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2017-11-10"; volume: "8:1521. doi"; pages: " 
            10.3389/fimmu.2017.01521. eCollection 2017"
COMMENT     Sequence Label: pSecTaq2-hGas6-Myc-6xHis
FEATURES             Location/Qualifiers
     source          1..7596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1185..1214
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1230..1247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1270..1287)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1276..1500
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1546..1974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1633..1652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1843..1864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1983..2003)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1988..2317
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2168..2303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2230..2249
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2366..3391
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2366..3391
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    3521..3642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3558..3577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3612..3631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3691..3707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3691..3707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3704..3726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3715..3731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3739..3745,3746..3763,3764..3769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(3922..3939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4093..4681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4173..4192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4852..5643,5644..5712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5475..5494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5713..5817)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5892..5911)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6083..6462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6463..6666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6617..6637
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6711..6730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6711..6729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6747..6756
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     6753..6812
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
ORIGIN
        1 gtgtgaagtc cttgtacctg ggccggatgt tcagtgggac ccccgtgatc cgactgcgct
       61 ttaagaggct gcagcccacc aggctggtag ctgagtttga cttccggacc tttgaccccg
      121 agggcatcct cctctttgcc ggaggccacc aggacagcac ctggatcgtg ctggccctga
      181 gagccggccg gctggagctg cagctgcgct acaacggtgt cggccgtgtc accagcagcg
      241 gcccggtcat caaccatggc atgtggcaga caatctctgt tgaggagctg gcgcggaatc
      301 tggtcatcaa ggtcaacagg gatgctgtca tgaaaatcgc ggtggccggg gacttgttcc
      361 aaccggagcg aggactgtat catctgaacc tgaccgtggg aggtattccc ttccatgaga
      421 aggacctcgt gcagcctata aaccctcgtc tggatggctg catgaggagc tggaactggc
      481 tgaacggaga agacaccacc atccaggaaa cggtgaaagt gaacacgagg atgcagtgct
      541 tctcggtgac ggagagaggc tctttctacc ccgggagcgg cttcgccttc tacagcctgg
      601 actacatgcg gacccctctg gacgtcggga ctgaatcaac ctgggaagta gaagtcgtgg
      661 ctcacatccg cccagccgca gacacaggcg tgctgtttgc gctctgggcc cccgacctcc
      721 gtgccgtgcc tctctctgtg gcactggtag actatcactc cacgaagaaa ctcaagaagc
      781 agctggtggt cctggccgtg gagcatacgg ccttggccct aatggagatc aaggtctgcg
      841 acggccaaga gcacgtggtc accgtctcgc tgagggacgg tgaggccacc ctggaggtgg
      901 acggcaccag gggccagagc gaggtgagcg ccgcgcagct gcaggagagg ctggccgtgc
      961 tcgagaggca cctgcggagc cccgtgctca cctttgctgg cggcctgcca gatgtgccgg
     1021 tgacttcagc gccagtcacc gcgttctacc gcggctgcat gacactggag gtcaaccgga
     1081 ggctgctgga cctggacgag gcggcgtaca agcacagcga catcacggcc cactcctgcc
     1141 cccccgtgga gcccgccgca gccgcggccg ctcgaggagg gcccgaacaa aaactcatct
     1201 cagaagagga tctgaatagc gccgtcgacc atcatcatca tcatcattga gtttaaaccc
     1261 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     1321 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     1381 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     1441 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     1501 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     1561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     1681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     1741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     1801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     1861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     1921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     1981 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2041 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     2101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     2161 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     2221 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     2281 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     2341 cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga
     2401 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     2461 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     2521 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     2581 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
     2641 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     2701 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     2761 gttcggccca tttggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     2821 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     2881 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     2941 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     3001 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     3061 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     3121 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     3181 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     3241 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     3301 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     3361 ccccagcact cgtccgaggg caaaggaata gcacgtgcta cgagatttcg attccaccgc
     3421 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3481 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     3541 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3601 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     3661 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3781 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5821 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     5881 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     5941 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     6001 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     6061 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     6121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     6181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     6241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     6301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     6361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     6421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     6481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     6541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     6601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     6661 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     6721 cactataggg agacccaagc tggctagcca ccatggagac agacacactc ctgctatggg
     6781 tactgctgct ctgggttcca ggttccactg gtgacgcgct gttgccggcg cgcgaggcca
     6841 cgcagttcct gcggcccagg cagcgccgcg cctttcaggt cttcgaggag gccaagcagg
     6901 gccacctgga gagggagtgc gtggaggagc tgtgcagccg cgaggaggcg cgggaggtgt
     6961 tcgagaacga ccccgagacg gattattttt acccaagata cttagactgc atcaacaagt
     7021 atgggtctcc gtacaccaaa aactcaggct tcgccacctg cgtgcaaaac ctgcctgacc
     7081 agtgcacgcc caacccctgc gataggaagg ggacccaagc ctgccaggac ctcatgggca
     7141 acttcttctg cctgtgtaaa gctggctggg ggggccggct ctgcgacaaa gatgtcaacg
     7201 aatgcagcca ggagaacggg ggctgcctcc agatctgcca caacaagccg ggtagcttcc
     7261 actgttcctg ccacagcggc ttcgagctct cctctgatgg caggacctgc caagacatag
     7321 acgagtgcgc agactcggag gcctgcgggg aggcgcgctg caagaacctg cccggctcct
     7381 actcctgcct ctgtgacgag ggctttgcgt acagctccca ggagaaggct tgccgagatg
     7441 tggacgagtg tctgcagggc cgctgtgagc aggtctgcgt gaactcccca gggagctaca
     7501 cctgccactg tgacgggcgt gggggcctca agctgtccca ggacatggac acctgtgagg
     7561 acatcttgcc gtgcgtgccc ttcagcgtgg ccaaga
//
