LOCUS       sequence-451566-        9292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a chimeric contruct having N-terminal human EGFR and 
            C-terminal human Axl.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Kimani SG, Kumar S, Davra V, Chang YJ, Kasikara C, Geng K, Tsou WI, 
            Wang S, Hoque M, Bohac A, Lewis-Antes A, De Lorenzo MS, Kotenko SV, 
            Birge RB
  TITLE     Normalization of TAM post-receptor signaling reveals a cell invasive
            signature for Axl tyrosine kinase.
  JOURNAL   Cell Commun Signal. 2016 Sep 6;14(1):19. doi: 
            10.1186/s12964-016-0142-1.
  PUBMED    27595981
REFERENCE   2  (bases 1 to 9292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2016-09-6"; volume: "14(1):19. doi"; pages: " 
            10.1186/s12964-016-0142-1"
COMMENT     Sequence Label: pEF2-EFGR-hAxl
FEATURES             Location/Qualifiers
     source          1..9292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     602..621
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(742..1071)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      756..891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(810..829)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1056..1076
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1085..1513)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1195..1216)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1407..1426
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1559..1783
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1772..1789
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        1809..1827
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1809..1826
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(5143..6321)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5152..6090
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5175..5195)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6609..6628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6703..6807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6808..6876,6877..7668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7026..7045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7839..8427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8328..8347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8581..8598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8715..8736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8751..8756,8757..8774,8775..8781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8789..8805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8794..8816
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8813..8829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8813..8829
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8878..8999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8889..8908)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8943..8962
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(9173..9292,1..675))
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(join(9284..9292,1..11))
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
       61 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
      121 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
      181 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
      241 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
      301 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
      361 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
      421 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
      481 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
      541 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
      601 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
      661 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc cgaaaatgga
      721 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
      781 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
      841 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
      901 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
      961 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     1021 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     1081 aattaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     1141 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     1201 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     1261 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     1321 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     1381 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     1441 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     1501 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
     1561 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
     1621 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
     1681 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
     1741 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga
     1801 gctctagcat ttaggtgaca ctatagaata gggccctcta gtaggaattc aggcaccatc
     1861 ctcctgccct ggggctgctg gggagcccct atcagcaggc tgagcggggc taggggttgt
     1921 ggaagggcag aggacatagc gtccagcagg atggacctca gccgcagtga ggcagctaca
     1981 ggaatcctta gggtctggct gggttggggg gtcagctcct cctgcagctc cagggggttc
     2041 aggataacct ccaccctcat ccatgttgac atagaggatt tcgtcaggct cctgggcagg
     2101 aggcaaggcc ttcagtgtgt tctccaaatc ttcccgcagc tctgtaaaac ttggccggtc
     2161 ctggggattt agctcccagc accgcgacat caaggcatac agtccatcca gacagtccgc
     2221 aggctgcttc aggcgatttc cccggcgcag atagtcataa atctcgctgt tctccacgcc
     2281 cggatatggg gtttggcctc ttgtggcaat ctcccacatt gtcaccccga aggaccacac
     2341 atcgctcttg ctggtgtaga cacggtcagc tagactctca atggcaatcc acttgactgg
     2401 catcttggcg atacgtccct ggcggtagta gtccccattg tagatcttct tggagagccc
     2461 gaagtccgcc acacacacgg acatgttctc attcagcatg cagttcctgg ccgccaggtc
     2521 ccggtgtatg aatctcttgg tactcagata ctccatgcca ctggcgatgt ctgccatgaa
     2581 cttcactagc atctgagtgg gcaggtacac tggctggtcc ccgagccggg aatagaggag
     2641 gaagctgtgt aggtctccat gtttcatgaa aggtaagatg accacaggtg ctgggaagct
     2701 ctctcgttca gaaccctgga aacagacacc gatgagcctc atgacgttgg gatggtcaaa
     2761 ttccttcatg cagaccgctt cactcaggaa atcctccagc tctgacctcg tgcagatggc
     2821 aatcttcatc gtcttcacag ccaccttgag gatggagtcg tcctggttga gctggccttc
     2881 catcacagct ccaaactctc cctctcccag agtcttcccc agggccacct tgtgccggtc
     2941 caccatcaca tcccgcagct tctccttcag ctcttcactg atgcccaagc tgttcaaggt
     3001 agcttcagtg gtccgacgac tgtaggactt gcgcacgcgg tacctgacta ccagttcacc
     3061 tctttccact gttggttcaa acacttctcc ataacgggtc tccttctttc gccggtggac
     3121 aaggaagaga gccaagatga ggacacaggc agcggccacg actgctccta gcagtactgc
     3181 tagccaggac gggatcttag gcccattcgt tggacagcct tcaagacctg gcccagtgca
     3241 tccgtaggtg cagtttggat ggcacaggtg gcacacatgg ccggcgtctg cgtacttcca
     3301 gaccagggtg ttgttttctc ccatgactcc tgccgggcag gtcttgacgc agtgggggcc
     3361 gtcaatgtag tgggcacact ggatacagtt gtctggtccc cgtcctgtgc aggtgatgtt
     3421 catggcctga ggcaggcact ctgggtggca ctgtatgcac tcagagttct ccacaaactc
     3481 ccttggctca ccctccagaa ggttgcactt gtccacgcat tccctgcctc ggctgacatt
     3541 ccggcaagag acgcagtccc tgggctccgg gccccagcag ccctcggggg agcacaaggc
     3601 atggcagacc tggcctgtgg ccttgcagct gttttcacct ctgttgctta taattttggt
     3661 tttctgaccg gaggtcccaa acagtttttt ccagtttatt gtatttgcat agcacaaatt
     3721 tttgtttcct gaaattatca catctccatc acttatctcc ttgagggagc gtaatcccaa
     3781 ggatgttatg ttcaggctga cgactgcaag agaaaactga ccatgttgct tggtcctgcc
     3841 gcgtatgatt tctaggttct caaaggcatg aaggtccgtc ctgttttcag gccaagcctg
     3901 aatcagcaaa aaccctgtga tttcctttac ggttttcaga atatccagtt cctgtggatc
     3961 cagaggagga gtatgtgtga aggagtcacc cctaaatgcc accggcagga tgtggagatc
     4021 gccactgatg gaggtgcagt ttttgaagtg tttaatattc gtagcattta tggagagtga
     4081 gtctttaaat tcaccaatac ctattccgtt acacactttg cggcaaggcc cttcgcactt
     4141 cttacacttg cggacgccgt cttcctccat ctcatagctg tcggccccac aggctcggac
     4201 gcacgagccg tgatctgtca ccacataatt acggggacac ttcttcacgc aggtggcacc
     4261 aaagctgtat ttgccctcgg ggttcacatc catctggtac gtggtggggt tgtagagcat
     4321 gagtgggggg caggtgtcct tgcacgtggc ttcgtctcgg aatttgcggc agaccaggca
     4381 gtcgctctcc cgggggcctg tgcagcctgc agcacactgg ttgtggcagc agtcactggg
     4441 ggacttgcca cggcagcgcc cggagcactg ctgggcacag atgattttgg tcagtttctg
     4501 gcagttctcc tctcctgcac cccagcagct cccattggga cagcttggat cacacttttg
     4561 gcagctgccc aggtggttct ggaagtccat cgacatgttg ctgagaaagt cactgctgac
     4621 tatgtcccgc cactggatgc tctccacgtt gcacagggca gggttgttgc tgaaccgcac
     4681 ggcgccatgc aggatttcct gtaaatttct catgggcagc tccttcagtc cggttttatt
     4741 tgcatcatag ttagataaga ctgctaaggc ataggaattt tcgtagtaca tatttcctct
     4801 gatgatctgc aggttttcca aaggaattcg ctccactgtg ttgagggcaa tgaggacata
     4861 accagccacc tcctggatgg tctttaagaa ggaaagatca taattcctct gcacataggt
     4921 aatttccaaa ttcccaagga ccacctcaca gttattgaac atcctctgga ggctgagaaa
     4981 atgatcttca aaagtgccca actgcgtgag cttgttactc gtgccttggc aaactttctt
     5041 ttcctccaga gcccgactcg ccgggcagag cgcagccagc agcgccagga gcgctgcccc
     5101 ggccgtcccg gagggtcgca tcgctggtac caagctaatt cctcacgaca cctgaaatgg
     5161 aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc caaactcaaa
     5221 aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt cagtctccac
     5281 ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta aagacgacgt
     5341 actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact ccgtggagtc
     5401 acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg actcacccgc
     5461 ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc cgggaagcgg
     5521 ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc agggcggggc
     5581 gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag actacccccg
     5641 tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc gctgggacgc
     5701 acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca gatcttggcc
     5761 cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc aaaaatttta
     5821 aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc agattcgcac
     5881 gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag gggctcctta
     5941 agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat caagaatcac
     6001 gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg taaagaggcc
     6061 aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc cgttgcgaaa
     6121 aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag
     6181 gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc
     6241 ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg tgcgctctgc
     6301 ccactgacgg gcaccggagc caattcccac tcctttcaag acctagaagg tccattagct
     6361 gcaaagattc ctctctgttt aaaactttat ccatctttgc aaagcttttt gcaaaagcct
     6421 agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
     6481 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
     6541 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
     6601 taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct
     6661 cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6721 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6781 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6841 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6901 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6961 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7021 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7081 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7141 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7201 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7261 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7321 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7381 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7441 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7501 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7561 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7621 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7681 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7741 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7801 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7861 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7921 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7981 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8041 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8101 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8161 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8221 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8281 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8341 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8401 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8461 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8521 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8581 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8641 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8701 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8761 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8821 agctatgacc atgattacgc caagctctag ctagaggtcg acggtataca gacatgataa
     8881 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     8941 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
     9001 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     9061 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     9121 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga
     9181 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     9241 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cg
//
