LOCUS       sequence-451594-       12163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 
            synNotch receptors with a constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, 
            Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok 
            M, Lim WA
  TITLE     Engineering synthetic suppressor T cells that execute locally 
            targeted immunoprotective programs.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4793. doi: 
            10.1126/science.adl4793. Epub 2024 Dec 6.
  PUBMED    39636990
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4793. doi"; pages: " 
            10.1126/science.adl4793. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..12163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             57..690
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    858..992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(882..901)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     936..955
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1417..1437)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1567..1632
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(2766..3095)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2780..2915
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3080..3100
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(3170..3187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3341..3929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3421..3440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4100..4891,4892..4960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4723..4742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4961..5065)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5133..5151
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5189..5211)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5311..5330
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5544..5563
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5853..5871
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5853..5870
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             5896..6129
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6219..6235
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6220..6236
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6239..6827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6345..6356
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6754..6774
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6863..7564)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(7585..8072)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7633..7652)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8014..8037
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(8157..8867)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8435..8454)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8704..8722
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    8886..9459
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9046..9065)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9275..9292
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(10437..10461)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    10687..10804
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11089..11133)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11318..11551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    join(12048..12163,1..10)
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct
       61 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
      121 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
      181 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt
      241 acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc
      301 ccaggccacg cctccctgga aagtccccag cggaaagtcc cttgtagcaa gctcgatatc
      361 agcagttctt gaagtactcc ggatgcagct ctcgggccac gtgatgaaat gctaggcggc
      421 tgtcaaacct ccactctaac acttctctct acgggtcatc catcccatgc aggctcacag
      481 ggtgtaacaa gctggtgttc tctcctttat tggcctcttc taccttatct ggctcaactg
      541 gtactagctt gtagcaccat ccaaaggtca gtggatatct gacccctggc cctggtgtgt
      601 agttctgcta atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt
      661 gtcttctttg ggagtgaatt agcccttcca actactaagt ttgtagtaca tatttaacaa
      721 atacaatttc tttaaaatga aaataattca gaggaatcac aggtttagag taaatgaaac
      781 cacaggtaat tggcagtggt aatagggtat ggggtgggaa gtttgggatg attttggtta
      841 gcttgagtta tccagttgat ccagacatga taagatacat tgatgagttt ggacaaacca
      901 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      961 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     1021 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     1081 gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattcct tattaacccc
     1141 tttacaaatt aaaaagctaa aggtacacaa tttttgagca tagttattaa tagcagacac
     1201 tctatgcctg tgtggagtaa gaaaaaacag tatgttatga ttataactgt tatgcctact
     1261 tataaaggtt acagaatatt tttccataat tttcttgtat agcagtgcag ctttttcctt
     1321 tgtggtgtaa atagcaaagc aagcaagagt tctattacta aacacagcat gactcaaaaa
     1381 acttagcaat tctgaaggaa agtccttggg gtcttctacc tttctcttct tttttggagg
     1441 agtagaatgt tgagagtcag cagtagcctc atcatcacta gatggcattt cttctgagca
     1501 aaacaggttt tcctcattaa aggcattcca ccactgctcc cattcatcag ttccataggt
     1561 tggaatctaa aatacacaaa caattagaat cagtagttta acacattata cacttaaaaa
     1621 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
     1681 acagaagtaa ggttccttca caaagatccg gggcccactc ataaatccag ttgccgccac
     1741 ggtagccaat caccgtatcg tataaatcat cgtcggtacg ttcggcatcg ctcatcacaa
     1801 tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc gcgccagatc cacatcagac
     1861 ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac tgcacaggcg
     1921 gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt ttaaagcggg
     1981 gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc agaatactta
     2041 ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa cgcccggcag tgccaggcgt
     2101 tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc ccacggctgt
     2161 tcaatccagg tatcttgcgg gatatcaaca acatagtcat caaccagcgg acgaccagcc
     2221 ggttttgcga agatggtgac aaagtgcgct tttggataca tttcacgaat cgcaaccgca
     2281 gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc gccttctgcg
     2341 cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat acaaacggta
     2401 tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtacaga ccgccacggc
     2461 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
     2521 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
     2581 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
     2641 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
     2701 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgtcca
     2761 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2821 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2881 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     2941 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     3001 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     3061 gagcctgggg actttccaca ccctaactga cacacattcc acagctgcat taatgaatcg
     3121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     5101 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     5161 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5221 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5281 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5341 accattcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     5401 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     5461 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     5521 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     5581 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatctggct agcgatgacc
     5641 ctgctgattg gttcgctgac catttccggg tgcgggacgg cgttaccaga aactcagaag
     5701 gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
     5761 agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
     5821 taatacataa ccttatgtat catacacata cgatttaggt gacactatag aatacacgga
     5881 attaattcta gatgctgcta gagattttcc acactgacta aaagggtctg agggatctct
     5941 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     6001 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     6061 ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta
     6121 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
     6181 tcattggtct taaaggtacc gagctcgaat taattccctc gaggtcgacg gtatcgatgc
     6241 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     6301 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     6361 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     6421 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     6481 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     6541 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     6601 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     6661 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     6721 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     6781 agaataccag tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg
     6841 atatcaagct tgcatgcctg cattaattaa gcttgtgccc cagtttgcta gggaggtcgc
     6901 agtatctggc cactgccacc tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga
     6961 ttctttccag tctgtagtcc acatagtaga cgccaggcat cttgaggttc ttagcgggtt
     7021 tcttggatct atatgtggtc ttgatgtttg cgatcagatg gctcccgccc acgagcttca
     7081 gggccatgtc gtttctgcct tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg
     7141 cctcccagcc gagtgttttc ttctgcatca cagggccgtt ggatgtgaag ttcacccctc
     7201 tgatcttgac gttgtagatg aggcagccgt cctggaggct ggtgtcctgg gtagcggtca
     7261 gcacgccccc gtcttcgtat gtggtgactc tctcccatgt gaagccctca gggaaggact
     7321 gcttgaagaa gtcggggatg ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga
     7381 agctagtagc caggatgtcg aaggcgaagg ggagagggcc gccctcgacc accttgattc
     7441 tcatggtctg ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat
     7501 ggttgtccac ggtgccctcc atgtacagct tcatgtgcat gttctcctta atcagctcgc
     7561 tcatggtaag cttgggctgc aggtcgaaag gcccggagat gaggaagagg agaacagcgc
     7621 ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc
     7681 cgccccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     7741 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     7801 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     7861 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     7921 gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     7981 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
     8041 agactgcctt gggaaaagcg cctcccctac ccggtagaat tcgttaacct cgtgcaggtc
     8101 gactctagag tcgcggcctc agtcagtcac gcatgttgca ggtgggagtt gcggccctac
     8161 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc
     8221 acgatggtgt agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg
     8281 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg
     8341 ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg
     8401 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
     8461 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag
     8521 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
     8581 aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag
     8641 gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca
     8701 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
     8761 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
     8821 aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatgga cccgccagcg
     8881 ctcatttatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga
     8941 cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat
     9001 cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag
     9061 agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc
     9121 tttgcaggtg tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg
     9181 gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga
     9241 ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga
     9301 cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa
     9361 gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta ttccaagcgg
     9421 cttcggccag taacgttagg ggggggggag ggagaggggg gccgcaagtc aaggatctca
     9481 tgtctcctcc aagtgtgtat cagactgttt tttactgttc gtatcttgga ttccgcactt
     9541 cttcacatcc atcatgcggc ctttgcgaag gcggaaaata aaagtcagtg ccacgccaag
     9601 acacaggagt atagcaccga gtatcaccag atgtgttctt tcgttggggg gatgcgcgag
     9661 gggcaactct ggtatcacaa gctctgccgt atgattttcc tccggatcga gtcgcctgaa
     9721 ggtgcaatag aaaatttcgt ttgtggttgt attgattctc agtgttgacg taacattaaa
     9781 cagcttctct tccctcttac tgttcgttgt tgtggtttta ccagacaaga cctggtggtc
     9841 tgaactcgtc catattacct ccgctttcgg gtaaccttcg gcttgacacg tcagctcatg
     9901 ttcgctggtc acaggatcca ccacaaggat ccgttggtta attttattgt agggcgcatt
     9961 cactttaacg gtaatgcgtt tatagtcagc accaccgtat gagatcatac atcggtaaac
    10021 tccggcatcc tgaagtttaa catccgttat ttgcaacgct gcattgccca agctaagctg
    10081 gtctttgagg aggcgagccc gctgtctata actactatgt tgcactttaa ggtcctcctc
    10141 tccgtggacg aattgaatga tatttttgtc ctccatttcc caataaacta tcaatgcggc
    10201 cagatccaac tgcttttcca cggggaactt gcattctatc gtcatattgc tgccgtactc
    10261 gacaacataa agatcctttg gcacggtgac cgtgaacgca ttcagaagat gccagtacgt
    10321 cataaagata aacacggcga aaattctcat ggcggcgatc caacgaatgt cgagaggctg
    10381 gatcggtccc ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc
    10441 tgacggttca ctaaacgagc tctgcttata taggcctccc accgtacacg cctaactagt
    10501 cgttcggagg acagtgctcc gacgttcgga ggacatgctc cgttcggagg acagtgctcc
    10561 gacgttcgga ggacagtgct ccgacgttcg gaggacagtg ctccgacgtt cggaggacag
    10621 tgctccgaat tcgatatcaa gcttatcgat ccaaactgga tctctgctgt ccctgtaata
    10681 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
    10741 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
    10801 aaaattgtgg atgaatactg ccatttggcg ataccgtcga gatccgttca ctaatcgaat
    10861 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc
    10921 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
    10981 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
    11041 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
    11101 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
    11161 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
    11221 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
    11281 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
    11341 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
    11401 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
    11461 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
    11521 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
    11581 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
    11641 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
    11701 cggccggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
    11761 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
    11821 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
    11881 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
    11941 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
    12001 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
    12061 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
    12121 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgc
//
