LOCUS       sequence-451595-       11827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 
            synNotch receptors with a constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, 
            Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok 
            M, Lim WA
  TITLE     Engineering synthetic suppressor T cells that execute locally 
            targeted immunoprotective programs.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4793. doi: 
            10.1126/science.adl4793. Epub 2024 Dec 6.
  PUBMED    39636990
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4793. doi"; pages: " 
            10.1126/science.adl4793. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-GAL4-IL10-IRES-mCherry-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..11827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    690..824
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(714..733)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     768..787
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1249..1269)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1399..1464
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(2598..2927)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2612..2747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2912..2932
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(3002..3019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3173..3761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3253..3272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3932..4723,4724..4792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4555..4574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4793..4897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4965..4983
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5021..5043)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5143..5162
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5376..5395
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5685..5703
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5685..5702
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             5728..5961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6051..6067
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6052..6068
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6071..6659
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6177..6188
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6586..6606
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6695..7396)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(7417..7904)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7465..7484)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7846..7869
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(7989..8699)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8267..8286)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8536..8554
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    8718..9291
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8878..8897)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9107..9124
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(9933..9957)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    10183..10300
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10585..10629)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10814..11047
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11544..11669
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(11716..11827,1..522)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
       61 gagagaccca gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact
      121 ccccagtccc gcccaggcca cgcctccctg gaaagtcccc agcggaaagt cccttgtagc
      181 aagctcgata tcagcagttc ttgaagtact ccggatgcag ctctcgggcc acgtgatgaa
      241 atgctaggcg gctgtcaaac ctccactcta acacttctct ctacgggtca tccatcccat
      301 gcaggctcac agggtgtaac aagctggtgt tctctccttt attggcctct tctaccttat
      361 ctggctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgacccctg
      421 gccctggtgt gtagttctgc taatcaggga agtagccttg tgtgtggtag atccacagat
      481 caaggatatc ttgtcttctt tgggagtgaa ttagcccttc caactactaa gtttgtagta
      541 catatttaac aaatacaatt tctttaaaat gaaaataatt cagaggaatc acaggtttag
      601 agtaaatgaa accacaggta attggcagtg gtaatagggt atggggtggg aagtttggga
      661 tgattttggt tagcttgagt tatccagttg atccagacat gataagatac attgatgagt
      721 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      781 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      841 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      901 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
      961 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1021 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1081 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1141 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1201 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1261 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1321 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1381 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1441 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1501 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cggggcccac tcataaatcc
     1561 agttgccgcc acggtagcca atcaccgtat cgtataaatc atcgtcggta cgttcggcat
     1621 cgctcatcac aatacgtgcc tggacgtcga ggatttcgcg tgggtcaatg ccgcgccaga
     1681 tccacatcag acggttaatc atgcgatacc agtgagggat ggttttacca tcaagggccg
     1741 actgcacagg cggttgtgcg ccgtgattaa agcggcggac tagcgtcgag gtttcaggat
     1801 gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg gatgcagcct
     1861 ccagaatact tactggaaac tattgtaacc cgcctgaagt taaaaagaac aacgcccggc
     1921 agtgccaggc gttgaaaaga ttagcgaccg gagattggcg ggacgaatac gacgcccata
     1981 tcccacggct gttcaatcca ggtatcttgc gggatatcaa caacatagtc atcaaccagc
     2041 ggacgaccag ccggttttgc gaagatggtg acaaagtgcg cttttggata catttcacga
     2101 atcgcaaccg cagtaccacc ggtatccacc aggtcatcaa taacgatgaa gccttcgcca
     2161 tcgccttctg cgcgtttcag cactttaagc tcgcgctggt tgtcgtgatc gtagctggaa
     2221 atacaaacgg tatcgacatg acgaataccc agttcacgcg ccagtaacgc acccggtaca
     2281 gaccgccacg gcttacggca ataatgcctt tccattgttc agaaggcatc agtcggcttg
     2341 cgagtttacg tgcatggatc tgcaacatgt cccaggtgac gatgtatttt tcgctcatgt
     2401 gaagtgtccc agcctgttta tctacggctt aaaaagtgtt cgaggggaaa ataggttgcg
     2461 cgagattata gagatctggc gcactaaaaa ccagtatttc acatgagtcc gcgtcttttt
     2521 acgcactgcc tctccctgac gcgggataaa gtggtattct caaacatatc tcgcaagcct
     2581 gtcttgtgtc caagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc
     2641 tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag
     2701 ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg
     2761 ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     2821 gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt
     2881 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgc
     2941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3901 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3961 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4021 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4081 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4141 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4201 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4261 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4321 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4381 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4441 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4501 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4561 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4621 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4681 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4741 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4921 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4981 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     5041 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     5101 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     5161 tactgagagt gcaccattcg acgctctccc ttatgcgact cctgcattag gaagcagccc
     5221 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
     5281 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
     5341 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
     5401 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg
     5461 ctagcgatga ccctgctgat tggttcgctg accatttccg ggtgcgggac ggcgttacca
     5521 gaaactcaga aggttcgtcc aaccaaaccg actctgacgg cagtttacga gagagatgat
     5581 agggtctgct tcagtaagcc agatgctaca caattaggct tgtacatatt gtcgttagaa
     5641 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
     5701 agaatacacg gaattaattc tagatgctgc tagagatttt ccacactgac taaaagggtc
     5761 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     5821 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     5881 ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt
     5941 gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag
     6001 ctgccttgta agtcattggt cttaaaggta ccgagctcga attaattccc tcgaggtcga
     6061 cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     6121 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     6181 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     6241 agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac
     6301 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     6361 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     6421 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     6481 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     6541 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     6601 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta
     6661 tcgataagct tgatatcaag cttgcatgcc tgcattaatt aagcttgtgc cccagtttgc
     6721 tagggaggtc gcagtatctg gccactgcca cctcgtgctg ctcgacgtag gtctcgttgt
     6781 tggcctcctt gattctttcc agtctgtagt ccacatagta gacgccaggc atcttgaggt
     6841 tcttagcggg tttcttggat ctatatgtgg tcttgatgtt tgcgatcaga tggctcccgc
     6901 ccacgagctt cagggccatg tcgtttctgc cttccaggcc gccgtcagcg gggtacagcg
     6961 tctcggtgaa ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatgtga
     7021 agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct
     7081 gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct
     7141 cagggaagga ctgcttgaag aagtcgggga tgccctgggt gtggttgatg aaggtcttgc
     7201 tgccgtagag gaagctagta gccaggatgt cgaaggcgaa ggggagaggg ccgccctcga
     7261 ccaccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc tcggatgtgc
     7321 acttgaagtg atggttgtcc acggtgccct ccatgtacag cttcatgtgc atgttctcct
     7381 taatcagctc gctcatggta agcttgggct gcaggtcgaa aggcccggag atgaggaaga
     7441 ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga
     7501 ccttcgggcg cccgccccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg
     7561 agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt
     7621 ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg
     7681 tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt
     7741 agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt
     7801 ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa
     7861 agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga attcgttaac
     7921 ctcgtgcagg tcgactctag agtcgcggcc tcagtcagtc acgcatgttg caggtgggag
     7981 ttgcggccct acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
     8041 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
     8101 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     8161 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
     8221 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
     8281 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
     8341 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     8401 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     8461 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     8521 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     8581 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
     8641 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatg
     8701 gacccgccag cgctcattta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc
     8761 ggggggccta gacgtttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc
     8821 cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat
     8881 acgcttgagg agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg
     8941 ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg
     9001 tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa
     9061 gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg
     9121 agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc
     9181 acattgccaa aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct
     9241 tattccaagc ggcttcggcc agtaacgtta gggggggggg agggagaggg gggccgcaag
     9301 tcaaggatct cagttgcgaa tcttcatagt catataagcc tcgatataat tgatgaagat
     9361 gtcaaattca ctcatcgcct tgtatatacc cttctcctgc aacttattga aggcgttttt
     9421 aacttgctca actgccttac tcttattttc gcacggcagg aatctgtggc agcgccgcaa
     9481 gcggagtctg agtgttttca ggttttcccc gagtgaattg acatgagctt ttatatcagg
     9541 gtcttgattt tcggcttgcg gcattacctc ttcaagatag aactggatca tttcagagag
     9601 cgcctgacag cccaagtatc ctttaaagtc ctccaacaaa gattctttca agagcagatt
     9661 atcaagttga tctttcatct ggaagaatgt cttcacccgg gaaaaggcat ctctcaggtc
     9721 ccgaagcatg ttcggcagat tgcctgggaa atgagtgcaa ctattttcag attgtgtacc
     9781 ttgacccggt gaggctcgaa ctccggtgag gagcaccaag caacacagaa gcgcggagct
     9841 atgcatggcg gcgatccaac gaatgtcgag aggctggatc ggtcccggtg tcttctatgg
     9901 aggtcaaaac agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg
     9961 cttatatagg cctcccaccg tacacgccta actagtcgtt cggaggacag tgctccgacg
    10021 ttcggaggac atgctccgtt cggaggacag tgctccgacg ttcggaggac agtgctccga
    10081 cgttcggagg acagtgctcc gacgttcgga ggacagtgct ccgaattcga tatcaagctt
    10141 atcgatccaa actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
    10201 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
    10261 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
    10321 ttggcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
    10381 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
    10441 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
    10501 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
    10561 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
    10621 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
    10681 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
    10741 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
    10801 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
    10861 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
    10921 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
    10981 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
    11041 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
    11101 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
    11161 gtccctcata tctcctcctc caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg
    11221 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
    11281 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
    11341 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
    11401 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
    11461 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
    11521 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
    11581 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
    11641 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
    11701 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
    11761 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
    11821 attgagg
//
