LOCUS       sequence-451596-       12619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 
            synNotch receptors with a constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12619)
  AUTHORS   Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, 
            Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok 
            M, Lim WA
  TITLE     Engineering synthetic suppressor T cells that execute locally 
            targeted immunoprotective programs.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4793. doi: 
            10.1126/science.adl4793. Epub 2024 Dec 6.
  PUBMED    39636990
REFERENCE   2  (bases 1 to 12619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4793. doi"; pages: " 
            10.1126/science.adl4793. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-GAL4-IL35-IRES-mCherry-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..12619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    113..238
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             285..918
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    1086..1220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1110..1129)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1164..1183
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1645..1665)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1795..1860
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(2994..3323)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3008..3143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3308..3328
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(3398..3415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3569..4157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3649..3668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4328..5119,5120..5188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4951..4970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5189..5293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5361..5379
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5417..5439)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5539..5558
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5772..5791
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        6081..6099
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6081..6098
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             6124..6357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6447..6463
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6448..6464
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6467..7055
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6573..6584
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6982..7002
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7091..7792)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(7813..8300)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7861..7880)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8242..8265
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(8385..9095)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8663..8682)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8932..8950
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    9114..9687
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9274..9293)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9503..9520
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(11121..11145)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    11371..11488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11773..11817)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12002..12235
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
       61 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
      121 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
      181 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
      241 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
      301 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
      361 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
      421 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag
      481 cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc
      541 tccctggaaa gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga
      601 agtactccgg atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc
      661 actctaacac ttctctctac gggtcatcca tcccatgcag gctcacaggg tgtaacaagc
      721 tggtgttctc tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt
      781 agcaccatcc aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat
      841 cagggaagta gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg
      901 agtgaattag cccttccaac tactaagttt gtagtacata tttaacaaat acaatttctt
      961 taaaatgaaa ataattcaga ggaatcacag gtttagagta aatgaaacca caggtaattg
     1021 gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat tttggttagc ttgagttatc
     1081 cagttgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1141 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1201 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     1261 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     1321 atgatctcta gtcaaggcac tatacatcaa atattcctta ttaacccctt tacaaattaa
     1381 aaagctaaag gtacacaatt tttgagcata gttattaata gcagacactc tatgcctgtg
     1441 tggagtaaga aaaaacagta tgttatgatt ataactgtta tgcctactta taaaggttac
     1501 agaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg tggtgtaaat
     1561 agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac ttagcaattc
     1621 tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag tagaatgttg
     1681 agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa acaggttttc
     1741 ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg gaatctaaaa
     1801 tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt ttatatttac
     1861 cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac agaagtaagg
     1921 ttccttcaca aagatccggg gcccactcat aaatccagtt gccgccacgg tagccaatca
     1981 ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct catcacaata cgtgcctgga
     2041 cgtcgaggat ttcgcgtggg tcaatgccgc gccagatcca catcagacgg ttaatcatgc
     2101 gataccagtg agggatggtt ttaccatcaa gggccgactg cacaggcggt tgtgcgccgt
     2161 gattaaagcg gcggactagc gtcgaggttt caggatgttt aaagcggggt ttgaacaggg
     2221 tttcgctcag gtttgcctgt gtcatggatg cagcctccag aatacttact ggaaactatt
     2281 gtaacccgcc tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg aaaagattag
     2341 cgaccggaga ttggcgggac gaatacgacg cccatatccc acggctgttc aatccaggta
     2401 tcttgcggga tatcaacaac atagtcatca accagcggac gaccagccgg ttttgcgaag
     2461 atggtgacaa agtgcgcttt tggatacatt tcacgaatcg caaccgcagt accaccggta
     2521 tccaccaggt catcaataac gatgaagcct tcgccatcgc cttctgcgcg tttcagcact
     2581 ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac aaacggtatc gacatgacga
     2641 atacccagtt cacgcgccag taacgcaccc ggtacagacc gccacggctt acggcaataa
     2701 tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag tttacgtgca tggatctgca
     2761 acatgtccca ggtgacgatg tatttttcgc tcatgtgaag tgtcccagcc tgtttatcta
     2821 cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag attatagaga tctggcgcac
     2881 taaaaaccag tatttcacat gagtccgcgt ctttttacgc actgcctctc cctgacgcgg
     2941 gataaagtgg tattctcaaa catatctcgc aagcctgtct tgtgtccaag ctttttgcaa
     3001 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
     3061 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     3121 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     3181 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     3241 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     3301 tttccacacc ctaactgaca cacattccac agctgcatta atgaatcggc caacgcgcgg
     3361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5341 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     5401 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5461 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5521 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cattcgacgc
     5581 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     5641 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     5701 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     5761 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     5821 atgccggcca cgatgcgtcc ggcgtagagg atctggctag cgatgaccct gctgattggt
     5881 tcgctgacca tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt tcgtccaacc
     5941 aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag taagccagat
     6001 gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta atacataacc
     6061 ttatgtatca tacacatacg atttaggtga cactatagaa tacacggaat taattctaga
     6121 tgctgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     6181 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     6241 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     6301 acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc ccttccagtc
     6361 cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc attggtctta
     6421 aaggtaccga gctcgaatta attccctcga ggtcgacggt atcgatgcgg ggaggcggcc
     6481 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
     6541 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
     6601 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat
     6661 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
     6721 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
     6781 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
     6841 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     6901 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     6961 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     7021 aatctttcac aaattttgta atccagaggt tgattatcga taagcttgat atcaagcttg
     7081 catgcctgca ttaattaagc ttgtgcccca gtttgctagg gaggtcgcag tatctggcca
     7141 ctgccacctc gtgctgctcg acgtaggtct cgttgttggc ctccttgatt ctttccagtc
     7201 tgtagtccac atagtagacg ccaggcatct tgaggttctt agcgggtttc ttggatctat
     7261 atgtggtctt gatgtttgcg atcagatggc tcccgcccac gagcttcagg gccatgtcgt
     7321 ttctgccttc caggccgccg tcagcggggt acagcgtctc ggtgaaggcc tcccagccga
     7381 gtgttttctt ctgcatcaca gggccgttgg atgtgaagtt cacccctctg atcttgacgt
     7441 tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccgt
     7501 cttcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc ttgaagaagt
     7561 cggggatgcc ctgggtgtgg ttgatgaagg tcttgctgcc gtagaggaag ctagtagcca
     7621 ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg
     7681 tgccctcgta gggcttgcct tcgccctcgg atgtgcactt gaagtgatgg ttgtccacgg
     7741 tgccctccat gtacagcttc atgtgcatgt tctccttaat cagctcgctc atggtaagct
     7801 tgggctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     7861 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     7921 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag
     7981 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat
     8041 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg
     8101 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc
     8161 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc
     8221 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg
     8281 gaaaagcgcc tcccctaccc ggtagaattc gttaacctcg tgcaggtcga ctctagagtc
     8341 gcggcctcag tcagtcacgc atgttgcagg tgggagttgc ggccctactt gtacagctcg
     8401 tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag
     8461 tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc
     8521 acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc
     8581 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
     8641 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
     8701 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
     8761 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
     8821 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
     8881 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc
     8941 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     9001 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
     9061 atggccatgt tatcctcctc gcccttgctc accatggacc cgccagcgct catttatcat
     9121 cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg tttttttaac
     9181 ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc catacaatgg
     9241 ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag ccatttgact
     9301 ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta
     9361 tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg gttccgctgc
     9421 ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg aactgcttcc
     9481 ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg
     9541 ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg
     9601 gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta
     9661 acgttagggg ggggggaggg agaggggggc cgcaagtcaa ggatctcatg aagcgttcag
     9721 gtaagacatc actctgtcaa ttgtcacggc tcttatgcgg aacgcgtgca gaagaatgca
     9781 gagtttgatc ttagtcttgt agaaatctgg ctcctcaagt gaagattttt gcgggacagt
     9841 ctcgctattg aaattcagtg cttgcatgag ttcatctatc accgcgagca tgttttggtc
     9901 aagaaagatt tgccttttgg ggtccatcag aagttttgcg ttcattgtct taaactcgac
     9961 ttggtacatt ttcaggtcct catagatgga actcaagcac agtgccatca taaatgatgt
    10021 tttccgacta gccagacagg aaccgttggt tatgaagctt gtctcgcgtg aattgaggca
    10081 ggattcattt tttgtaagct ccaggggaag gcacgcttcg acagtggacg tcttgtcctt
    10141 agtaatatcc tcatggtcaa tttcctctga agtacatgga taaaactcca aagtttgccg
    10201 ggctttctga agcatatttg aaacggcgcg gagcaagttt tgtgaatggt gcaagcaggg
    10261 gaacatgcca ggatctggag ttgccaccgg aaggttccgg gagccaccac cgctaccccc
    10321 cccacttccg ccgccgctcc cacctccttt accgaggctc atggtcgcag tagctggcaa
    10381 ggaccaatct gagagttctc cgtaatcagt aagatcttgg gctgcaactt ggacataata
    10441 ccgagctcgc ggtctcactg ctcgcaatat aaaggaagta gcctcaatcg ggcctactcg
    10501 atgaaagcga gcagcacctt ggcgtttata tcgaatccag tatttcaagc taaatatctc
    10561 tgggaacggc cagctgcccg gtggttccca ttgaacttgg agctgccgct ccgcgagcgg
    10621 ggacagtcta actccttctg gagggtcggg cttgataata tgctccgtga taaaaggtac
    10681 aaagctactg ctggagcccc aggggtgtac cgctgttacg ttcaatacgt agggggccat
    10741 gctaaacaat tgaacatcag tgatagtaca ggacgtgctt gtaggggtct gctgcaagca
    10801 gggccagcta tgaccgcggg cggccatgcc caaccgataa gtagcgatga aactgaccgg
    10861 tgatgttgaa tttggtgccg ggggcaatgt ccatgaacaa tctacagcta tcggatatct
    10921 actagcccgg cactgcacgc ggggcaacgt caaggcggcg ggagggcctt ttctgccaga
    10981 gcaaggtggg caagatgccc agagcacaag cgccaaaagc agttgagggg tcatggcggc
    11041 gatccaacga atgtcgagag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag
    11101 cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc
    11161 tcccaccgta cacgcctaac tagtcgttcg gaggacagtg ctccgacgtt cggaggacat
    11221 gctccgttcg gaggacagtg ctccgacgtt cggaggacag tgctccgacg ttcggaggac
    11281 agtgctccga cgttcggagg acagtgctcc gaattcgata tcaagcttat cgatccaaac
    11341 tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
    11401 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
    11461 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt ggcgataccg
    11521 tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc
    11581 tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
    11641 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
    11701 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
    11761 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
    11821 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
    11881 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
    11941 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
    12001 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
    12061 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
    12121 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
    12181 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
    12241 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
    12301 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
    12361 tcctcctcca ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt cttacttttg
    12421 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
    12481 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
    12541 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
    12601 ccaggattaa ctgcgaatc
//
