LOCUS       sequence-451597-       11280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - mCherry reporter with a BAMH1 site for 
            Gal4DBDVP64 synNotch receptors with a constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, 
            Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok 
            M, Lim WA
  TITLE     Engineering synthetic suppressor T cells that execute locally 
            targeted immunoprotective programs.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4793. doi: 
            10.1126/science.adl4793. Epub 2024 Dec 6.
  PUBMED    39636990
REFERENCE   2  (bases 1 to 11280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4793. doi"; pages: " 
            10.1126/science.adl4793. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..11280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             13..246
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     336..352
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     337..353
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    356..944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             462..473
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     871..891
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(980..1681)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(1702..2189)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1750..1769)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2131..2154
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(2274..2984)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2552..2571)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2821..2839
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    3003..3576
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3163..3182)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3392..3409
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(3671..3695)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3921..4038
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4323..4367)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4552..4785
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5282..5407
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5454..6087
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6255..6389
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6279..6298)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6333..6352
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6814..6834)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6964..7029
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(8163..8492)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8177..8312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8477..8497
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8567..8584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8738..9326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8818..8837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9497..10288,10289..10357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10120..10139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10358..10462)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10530..10548
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10586..10608)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10708..10727
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10941..10960
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        11250..11268
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     11250..11267
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aattctagat gctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
       61 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
      121 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
      181 accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc
      241 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
      301 ttggtcttaa aggtaccgag ctcgaattaa ttccctcgag gtcgacggta tcgatgcggg
      361 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
      421 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
      481 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag
      541 gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
      601 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
      661 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
      721 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
      781 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
      841 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
      901 ataccagtca atctttcaca aattttgtaa tccagaggtt gattatcgat aagcttgata
      961 tcaagcttgc atgcctgcat taattaagct tgtgccccag tttgctaggg aggtcgcagt
     1021 atctggccac tgccacctcg tgctgctcga cgtaggtctc gttgttggcc tccttgattc
     1081 tttccagtct gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct
     1141 tggatctata tgtggtcttg atgtttgcga tcagatggct cccgcccacg agcttcaggg
     1201 ccatgtcgtt tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct
     1261 cccagccgag tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga
     1321 tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca
     1381 cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct
     1441 tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc
     1501 tagtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca
     1561 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt
     1621 tgtccacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctca
     1681 tggtaagctt gggctgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc
     1741 agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc
     1801 cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa
     1861 ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt
     1921 gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga
     1981 cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa
     2041 ggggccacca aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg
     2101 ccagaggcca cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga
     2161 ctgccttggg aaaagcgcct cccctacccg gtagaattcg ttaacctcgt gcaggtcgac
     2221 tctagagtcg cggcctcagt cagtcacgca tgttgcaggt gggagttgcg gccctacttg
     2281 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg
     2341 atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg
     2401 ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg
     2461 ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac
     2521 atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag
     2581 gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg cagggaggag
     2641 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag
     2701 ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt cacgtaggcc
     2761 ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag ggggccaccc
     2821 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
     2881 tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac
     2941 tccttgatga tggccatgtt atcctcctcg cccttgctca ccatggaccc gccagcgctc
     3001 atttatcatc gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt
     3061 ttttttaacc tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc
     3121 atacaatggg gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc
     3181 catttgactc tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt
     3241 gcaggtgtat cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg
     3301 ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc ttccagagga
     3361 actgcttcct tcacgacatt caacagacct tgcattcctt tggcgagagg ggaaagaccc
     3421 ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg ccctcacatt gccaaaagac
     3481 ggcaatatgg tggaaaataa catatagaca aacgcacacc ggccttattc caagcggctt
     3541 cggccagtaa cgttaggggg gggggaggga gaggggggcc gcaagtcaag gatccaacga
     3601 atgtcgagag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc
     3661 gtctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta
     3721 cacgcctaac tagtcgttcg gaggacagtg ctccgacgtt cggaggacat gctccgttcg
     3781 gaggacagtg ctccgacgtt cggaggacag tgctccgacg ttcggaggac agtgctccga
     3841 cgttcggagg acagtgctcc gaattcgata tcaagcttat cgatccaaac tggatctctg
     3901 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     3961 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     4021 cccccttttc ttttaaaatt gtggatgaat actgccattt ggcgataccg tcgagatccg
     4081 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
     4141 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     4201 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     4261 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     4321 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     4381 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     4441 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     4501 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     4561 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     4621 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     4681 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     4741 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     4801 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     4861 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     4921 ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     4981 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     5041 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     5101 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     5161 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     5221 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     5281 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     5341 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     5401 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     5461 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     5521 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     5581 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     5641 ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca
     5701 ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc
     5761 agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt
     5821 caaacctcca ctctaacact tctctctacg ggtcatccat cccatgcagg ctcacagggt
     5881 gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta
     5941 ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt
     6001 tctgctaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc
     6061 ttctttggga gtgaattagc ccttccaact actaagtttg tagtacatat ttaacaaata
     6121 caatttcttt aaaatgaaaa taattcagag gaatcacagg tttagagtaa atgaaaccac
     6181 aggtaattgg cagtggtaat agggtatggg gtgggaagtt tgggatgatt ttggttagct
     6241 tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     6301 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6361 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6421 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6481 tggctgatta tgatctctag tcaaggcact atacatcaaa tattccttat taaccccttt
     6541 acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag cagacactct
     6601 atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat gcctacttat
     6661 aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt
     6721 ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact
     6781 tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt
     6841 agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa
     6901 caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg
     6961 aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac ttaaaaattt
     7021 tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca
     7081 gaagtaaggt tccttcacaa agatccgggg cccactcata aatccagttg ccgccacggt
     7141 agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc atcacaatac
     7201 gtgcctggac gtcgaggatt tcgcgtgggt caatgccgcg ccagatccac atcagacggt
     7261 taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc acaggcggtt
     7321 gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta aagcggggtt
     7381 tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga atacttactg
     7441 gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc caggcgttga
     7501 aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca cggctgttca
     7561 atccaggtat cttgcgggat atcaacaaca tagtcatcaa ccagcggacg accagccggt
     7621 tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc aaccgcagta
     7681 ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc ttctgcgcgt
     7741 ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca aacggtatcg
     7801 acatgacgaa tacccagttc acgcgccagt aacgcacccg gtacagaccg ccacggctta
     7861 cggcaataat gcctttccat tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat
     7921 ggatctgcaa catgtcccag gtgacgatgt atttttcgct catgtgaagt gtcccagcct
     7981 gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat
     8041 ctggcgcact aaaaaccagt atttcacatg agtccgcgtc tttttacgca ctgcctctcc
     8101 ctgacgcggg ataaagtggt attctcaaac atatctcgca agcctgtctt gtgtccaagc
     8161 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     8221 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     8281 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     8341 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     8401 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     8461 cctggggact ttccacaccc taactgacac acattccaca gctgcattaa tgaatcggcc
     8521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9781 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10081 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10501 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10561 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10621 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10681 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10741 attcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
    10801 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
    10861 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
    10921 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
    10981 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc gatgaccctg
    11041 ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac tcagaaggtt
    11101 cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt ctgcttcagt
    11161 aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg ctacaattaa
    11221 tacataacct tatgtatcat acacatacga tttaggtgac actatagaat acacggaatt
//
