LOCUS       sequence-451598-       12640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - mCherry reporter and sTNFaR for Gal4DBDVP64 
            synNotch receptors with a constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, 
            Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok 
            M, Lim WA
  TITLE     Engineering synthetic suppressor T cells that execute locally 
            targeted immunoprotective programs.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4793. doi: 
            10.1126/science.adl4793. Epub 2024 Dec 6.
  PUBMED    39636990
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4793. doi"; pages: " 
            10.1126/science.adl4793. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-GAL4-UAS—sTNFaR-IRES-mCherry-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..12640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(20..31)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(140..156)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(141..157)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             247..480
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(505..523)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(506..523)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(813..832)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1046..1065)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1165..1187
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1225..1243)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1311..1415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1416..1484,1485..2276)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1634..1653)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2447..3035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2936..2955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3189..3206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3276..3296)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3281..3610
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3461..3596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4744..4809
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4939..4959
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5384..5518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5421..5440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5475..5494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5686..6319
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6366..6491
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6988..7221
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7406..7450
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7735..7852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     8078..8102
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8183..8192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8822..8839
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     misc_feature    9557..10130
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9724..9741)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9951..9970
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10149..10859
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10294..10312)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10562..10581
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        10944..11431
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10979..11002)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11364..11383
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11452..12153
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    join(12189..12640,1..137)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12242..12262)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
       61 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
      121 ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg agctcggtac
      181 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
      241 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
      301 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
      361 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
      421 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
      481 gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca
      541 taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt
      601 agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg
      661 tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag
      721 cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg
      781 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
      841 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
      901 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
      961 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     1021 agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     1081 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     1141 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     1201 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1261 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1501 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1561 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1621 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     1681 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1741 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1801 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1861 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1921 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     1981 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2041 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2101 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2161 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2221 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2281 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3241 tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg
     3301 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3361 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3421 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3481 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3541 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3601 cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt
     3661 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     3721 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     3781 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3841 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3901 gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg
     3961 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct
     4021 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt
     4081 ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
     4141 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca
     4201 agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
     4261 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt
     4321 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
     4381 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
     4441 atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt
     4501 atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg
     4561 acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata
     4621 cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag
     4681 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     4741 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     4801 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     4861 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     4921 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     4981 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     5041 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     5101 tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt ttttcttact
     5161 ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc
     5221 tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat
     5281 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     5341 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     5401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     5521 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     5581 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     5641 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     5701 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5761 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     5821 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     5881 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccgtagaga gaagtgttag
     5941 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     6001 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     6061 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     6121 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6181 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6241 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6301 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     6361 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     6421 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     6481 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     6541 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     6601 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     6661 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     6721 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     6781 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     6841 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     6901 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     6961 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     7021 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     7081 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     7141 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     7201 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     7261 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     7321 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     7381 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     7441 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     7501 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     7561 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     7621 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     7681 tcgattagtg aacggatctc gacggtatcg ccaaatggca gtattcatcc acaattttaa
     7741 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     7801 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     7861 ttacagggac agcagagatc cagtttggat cgataagctt gatatcgaat tcggagcact
     7921 gtcctccgaa cgtcggagca ctgtcctccg aacgtcggag cactgtcctc cgaacgtcgg
     7981 agcactgtcc tccgaacgga gcatgtcctc cgaacgtcgg agcactgtcc tccgaacgac
     8041 tagttaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga
     8101 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
     8161 gcctctcgac attcgttgga tcgccgccat gggcctctcc accgtgcctg acctgctgct
     8221 gccgctggtg ctcctggagc tgttggtggg aatatacccc tcaggggtta ttggactggt
     8281 ccctcaccta ggggacaggg agaagagaga tagtgtgtgt ccccaaggaa aatatatcca
     8341 ccctcaaaat aattcgattt gctgtaccaa gtgccacaaa ggaacctact tgtacaatga
     8401 ctgtccaggc ccggggcagg atacggactg cagggagtgt gagagcggct ccttcaccgc
     8461 ttcagaaaac cacctcagac actgcctcag ctgctccaaa tgccgaaagg aaatgggtca
     8521 ggtggagatc tcttcttgca cagtggaccg ggacaccgtg tgtggctgca ggaagaacca
     8581 gtaccggcat tattggagtg aaaacctttt ccagtgcttc aattgcagcc tctgcctcaa
     8641 tgggaccgtg cacctctcct gccaggagaa acagaacacc gtgtgcacct gccatgcagg
     8701 tttctttcta agagaaaacg agtgtgtctc ctgtagtaac tgtaagaaaa gcctggagtg
     8761 cacgaagttg tgcctacccc agattgagaa tgttaagggc actgaggact caggcaccac
     8821 actggttccg cgtggatccg agcccaagtc ctgtgataag actcatacat gtccgccctg
     8881 cccggctccc gaacttctcg gcggcccctc agttttcctc ttccccccca aaccgaaaga
     8941 cacactgatg atcagtagga ccccggaggt aacctgcgta gttgtcgatg tcagccatga
     9001 ggacccggaa gttaaattta attggtatgt cgatggtgtg gaagtacata atgcgaaaac
     9061 taagcctcgg gaggagcagt acaatagtac ctatcgggtg gtttccgtcc tgaccgttct
     9121 gcatcaggac tggcttaacg gaaaggaata caagtgcaag gtcagtaata aggcactgcc
     9181 tgctccgatt gaaaaaacaa tatcaaaggc taaagggcag ccgcgcgagc cccaggtcta
     9241 cacgcttccc cctagtaggg aggaaatgac aaaaaatcaa gtctcactta cttgcttggt
     9301 taaggggttc tacccatcag acatcgcagt agaatgggag agcaacgggc aaccggaaaa
     9361 caactacaaa acgacacccc cagtactgga ttctgatggt agttttttct tgtactcaaa
     9421 gctgaccgtg gataaaagta ggtggcaaca gggaaatgtt ttctcttgct cagtcatgca
     9481 tgaggcactg cataaccatt acacacagaa gtcattgagc ttgagcccgg gcaagtgaga
     9541 tccttgactt gcggcccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     9601 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     9661 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     9721 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     9781 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     9841 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     9901 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     9961 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
    10021 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
    10081 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa atgagcgctg
    10141 gcgggtccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
    10201 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
    10261 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
    10321 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
    10381 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
    10441 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
    10501 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
    10561 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
    10621 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
    10681 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
    10741 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
    10801 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtagg
    10861 gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct
    10921 gcacgaggtt aacgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    10981 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    11041 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    11101 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    11161 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    11221 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    11281 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg gggcgggcgc
    11341 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
    11401 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgagcgag
    11461 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
    11521 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
    11581 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
    11641 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
    11701 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
    11761 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
    11821 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
    11881 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
    11941 ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc
    12001 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
    12061 gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac
    12121 ctccctagca aactggggca caagcttaat taatgcaggc atgcaagctt gatatcaagc
    12181 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
    12241 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
    12301 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
    12361 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
    12421 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
    12481 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
    12541 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
    12601 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
//
