LOCUS       sequence-45160-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Sims JJ, Scavone F, Cooper EM, Kane LA, Youle RJ, Boeke JD, Cohen RE
  TITLE     Polyubiquitin-sensor proteins reveal localization and linkage-type 
            dependence of cellular ubiquitin signaling.
  JOURNAL   Nat Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888.
  PUBMED    22306808
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888."
COMMENT     Sequence Label: pQCXIP-NLS-Vx3-mEGFP
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(285..305)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(330..350)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(375..401)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(375..401)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      410..419
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(540..564)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tcctcgccct tgctcaccat ggtggcgacc ggtggatccc cagcagagtt acgggactcc
       61 ttcaggctca gctcgatggc cttgcggatc agctcctcct cgtcctcttc ctgagcgtta
      121 acttcacgag attctttcag agacagttcg atagctttac ggatcagttc ttcttcgtct
      181 tctttaactt tttcttcagc acgagcttca cgcagagatt cctggatagc agcttttaaa
      241 tcaggatctt cttcttcttc catagcggtc attttagaag cttcaacttt acgttttttt
      301 ttcggagagg cggtcatctt gctggcctcc accttgcgct tcttcttggg gctcatctcg
      361 aggccgctgc cgccggcgta gtcgggcacg tcgtaggggt agctgccctc catggtggcg
      421 gcgcggccgc ggatcaattc ctgcaggtcc gttccaatgc accgttcccg gccgcggagg
      481 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
      541 atctgacggt tcactaaacg agctctgctt atatagacct ccc
//
