LOCUS       sequence-451600-       12463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 
            synNotch receptors with a constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Reddy NR, Maachi H, Xiao Y, Simic MS, Yu W, Tonai Y, Cabanillas DA, 
            Serrano-Wu E, Pauerstein PT, Tamaki W, Allen GM, Parent AV, Hebrok 
            M, Lim WA
  TITLE     Engineering synthetic suppressor T cells that execute locally 
            targeted immunoprotective programs.
  JOURNAL   Science. 2024 Dec 6;386(6726):eadl4793. doi: 
            10.1126/science.adl4793. Epub 2024 Dec 6.
  PUBMED    39636990
REFERENCE   2  (bases 1 to 12463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-12-6"; volume: "386(6726):eadl4793. doi"; pages: " 
            10.1126/science.adl4793. Epub 2024 Dec 6"
COMMENT     Sequence Label: pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP
FEATURES             Location/Qualifiers
     source          1..12463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(52..68)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(53..69)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             159..392
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(417..435)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(418..435)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(725..744)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(958..977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1077..1099
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1137..1155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1223..1327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1328..1396,1397..2188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1546..1565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2359..2947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2848..2867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3101..3118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3188..3208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3193..3522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3373..3508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4656..4721
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4851..4871
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5296..5430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5333..5352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5387..5406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5598..6231
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6278..6403
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6900..7133
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7318..7362
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7647..7764
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     7990..8014
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    9292..9865
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9459..9476)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9686..9705
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9884..10594
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10029..10047)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10297..10316
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        10679..11166
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10714..10737)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11099..11118
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11187..11888
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    join(11924..12463,1..49)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11977..11997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12395..12406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
       61 cgacctcgag ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc
      121 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
      181 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
      241 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      301 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      361 acccttttag tcagtgtgga aaatctctag cagcatctag aattaattcc gtgtattcta
      421 tagtgtcacc taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt
      481 tctaacgaca atatgtacaa gcctaattgt gtagcatctg gcttactgaa gcagacccta
      541 tcatctctct cgtaaactgc cgtcagagtc ggtttggttg gacgaacctt ctgagtttct
      601 ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa tcagcagggt catcgctagc
      661 cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      721 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      781 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
      841 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
      901 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca ctctcagtac
      961 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     1021 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     1081 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     1141 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     1201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1561 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1861 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3001 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3061 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3121 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3181 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     3241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     3301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     3361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3481 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg acacaagaca
     3541 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
     3601 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
     3661 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
     3721 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
     3781 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
     3841 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
     3901 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
     3961 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt
     4021 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
     4081 ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat
     4141 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
     4201 ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga
     4261 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
     4321 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
     4381 ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
     4441 ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg tgatgagcga
     4501 tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg gcggcaactg
     4561 gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt gtgacataat
     4621 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     4681 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     4741 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     4801 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     4861 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     4921 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     4981 cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca
     5041 gttataatca taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta
     5101 ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg
     5161 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     5221 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     5281 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5341 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5401 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     5461 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     5521 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     5581 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     5641 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     5701 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     5761 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     5821 tgggatggat gacccgtaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     5881 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     5941 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     6001 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     6241 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     6301 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     6361 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     6421 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     6481 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     6541 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     6601 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     6661 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     6721 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     6781 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     6841 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     6901 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     6961 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     7021 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     7081 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     7141 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     7201 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     7261 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     7321 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     7381 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     7441 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     7501 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     7561 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     7621 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     7681 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     7741 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     7801 atcgataagc ttgatatcga attcggagca ctgtcctccg aacgtcggag cactgtcctc
     7861 cgaacgtcgg agcactgtcc tccgaacgtc ggagcactgt cctccgaacg gagcatgtcc
     7921 tccgaacgtc ggagcactgt cctccgaacg actagttagg cgtgtacggt gggaggccta
     7981 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     8041 tgacctccat agaagacacc gggaccgatc cagcctctcg acattcgttg gatcgccgcc
     8101 atgccgccct cggggctgcg gctactgccg cttctgctcc cactcccgtg gcttctagtg
     8161 ctgacgcccg ggaggccagc cgcgggactc tccacctgca agaccatcga catggagctg
     8221 gtgaaacgga agcgcatcga agccatccgt ggccagatcc tgtccaaact aaggctcgcc
     8281 agtcccccaa gccaggggga ggtaccgccc ggcccgctgc ccgaggcggt gctcgctttg
     8341 tacaacagca cccgcgaccg ggtggcaggc gagagcgccg acccagagcc ggagcccgaa
     8401 gcggactact atgctaaaga ggtcacccgc gtgctaatgg tggaccgcaa caacgccatc
     8461 tatgagaaaa ccaaagacat ctcacacagt atatatatgt tcttcaatac gtcagacatt
     8521 cgggaagcag tgcccgaacc cccattgctg tcccgtgcag agctgcgctt gcagagatta
     8581 aaatcaagtg tggagcaaca tgtggaactc taccagaaat atagcaacaa ttcctggcgt
     8641 taccttggta accggctgct gacccccact gatacgcctg agtggctgtc ttttgacgtc
     8701 actggagttg tacggcagtg gctgaaccaa ggagacggaa tacagggctt tcgattcagc
     8761 gctcactcgt cttccgacag caaagataac aaactccacg tggaaatcaa cgggatcagc
     8821 cccaaacgtc ggggcgacct gggcaccatc catgacatga accggccctt cctgctcctc
     8881 atggccaccc ccctggaaag ggcccagcac ctgcacagct cacggcaccg gagagccctg
     8941 gataccaact attgcttcag ctccacagag aagaactgct gtgtgcggca gctgtacatt
     9001 gactttagga aggacctggg ttggaagtgg atccacgagc ccaagggcta ccatgccaac
     9061 ttctgtctgg gaccctgccc ctatatttgg agcctggaca cacagtacag caaggtcctt
     9121 gccctctaca accaacacaa cccgggcgct tcggcgtcac cgtgctgcgt gccgcaggct
     9181 ttggagccac tgcccatcgt ctactacgtg ggtcgcaagc ccaaggtgga gcagttgtcc
     9241 aacatgattg tgcgctcctg caagtgcagc tgagatcctt gacttgcggc ccccctctcc
     9301 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     9361 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     9421 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     9481 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     9541 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     9601 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     9661 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     9721 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     9781 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     9841 tttcctttga aaaacacgat gataaatgag cgctggcggg tccatggtga gcaagggcga
     9901 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     9961 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
    10021 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
    10081 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
    10141 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
    10201 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
    10261 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
    10321 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
    10381 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
    10441 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
    10501 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
    10561 ctccaccggc ggcatggacg agctgtacaa gtagggccgc aactcccacc tgcaacatgc
    10621 gtgactgact gaggccgcga ctctagagtc gacctgcacg aggttaacga attctaccgg
    10681 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
    10741 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
    10801 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
    10861 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
    10921 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
    10981 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
    11041 gggtccgggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
    11101 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
    11161 ctttcgacct gcagcccaag cttaccatga gcgagctgat taaggagaac atgcacatga
    11221 agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag
    11281 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
    11341 ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc
    11401 acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga
    11461 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
    11521 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg
    11581 tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg
    11641 gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg
    11701 caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg
    11761 tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg
    11821 agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc
    11881 ttaattaatg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
    11941 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    12001 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    12061 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    12121 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    12181 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    12241 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    12301 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    12361 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    12421 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctt
//
