LOCUS       sequence-45163-e         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Sims JJ, Scavone F, Cooper EM, Kane LA, Youle RJ, Boeke JD, Cohen RE
  TITLE     Polyubiquitin-sensor proteins reveal localization and linkage-type 
            dependence of cellular ubiquitin signaling.
  JOURNAL   Nat Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888.
  PUBMED    22306808
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888."
COMMENT     Sequence Label: pQCXIP-Rx3(A8)-mEGFP
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      252..261
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(382..406)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(403..606)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(432..452)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggtggcgacc ggtgganccc cagaaggccg gcaactattc agactttctg caatggcttt
       61 cctcaagagc tcttcttcct cctcggcagc cgccgctgcg gcagccgcag cttcctgttc
      121 actcattttg agagccagag caaactgttc ttcttcctct tctgcggcag cagcggcagc
      181 cgccgccgcc tcttgctcgc tcatcttcag tgcgagtgcg aattgctcct cctcggtcat
      241 atggctgccc tccatggtgg cggcgcggcc gcggatcaat tcctgcaggt ccgttccaat
      301 gcaccgttcc cggccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
      361 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac
      421 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
      481 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
      541 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
      601 catcaccatg gt
//
