LOCUS       sequence-451633-        1716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the secretion signal peptide sequence of the human 
            BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI 
            restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: SP_H6
FEATURES             Location/Qualifiers
     source          1..1716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..734
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(141..160)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      805..839
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      complement(1021..1609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1101..1120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
       61 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      121 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      181 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      241 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
      301 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      361 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt
      421 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
      481 aagggtttat tgagaatatg tttttcgttt cagccaatcc ctgggtgagt ttcaccagtt
      541 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
      601 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
      661 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc
      721 agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga tccagtaatg
      781 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
      841 ggtctggtct caaggggctc cacatacggc aacaacactc aaggccgcca cacaccaagc
      901 catccacata tgagaccgac tacggttatc cacagaatca ggggataacg caggaaagaa
      961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1201 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1441 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1681 catgattgat cgggcacgta agaggttcca actttc
//
