LOCUS       sequence-451638-        1731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the secretion signal peptide sequence of the yeast 
            FIG2 gene for use in Combinatorial Golden Gate Assembly (BsaI 
            restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: SP_B2
FEATURES             Location/Qualifiers
     source          1..1731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(96..130)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(201..860)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     775..794
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1042..1630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1531..1550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgaactctt ttgcctcctt agggcttatt tattctgttg tgaacctact
       61 aacaagggtc gaagcggccc cttgagacca gaccaataaa aaacgcccgg cggcaaccga
      121 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
      181 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      241 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
      301 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
      361 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
      421 gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
      481 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
      541 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
      601 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
      661 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
      721 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
      781 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
      841 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
      901 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
      961 atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1021 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1081 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1141 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1201 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1261 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1381 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1441 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1501 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1561 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1621 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1681 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//
