LOCUS       sequence-451646-        1761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the secretion signal peptide sequence of the human 
            LRP8 gene for use in Combinatorial Golden Gate Assembly (BsaI 
            restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: SP_B4
FEATURES             Location/Qualifiers
     source          1..1761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(126..160)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(231..890)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     805..824
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1072..1660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1561..1580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgggccttc ccgagccagg accacttcgt ctgttggccc ttttattatt
       61 actgttacta ttactacttc ttcagttaca gcatttggcg gcggctgccc cttgagacca
      121 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      181 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      241 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      301 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      361 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      421 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
      481 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
      541 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
      601 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
      661 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
      721 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
      781 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
      841 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
      901 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
      961 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1681 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1741 tgattctgtg gataaccgta g
//
