LOCUS       sequence-451693-        1755 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the secretion signal peptide sequence of the human 
            CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI 
            restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: SP_A4
FEATURES             Location/Qualifiers
     source          1..1755
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(120..154)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(225..884)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     799..818
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1066..1654
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1555..1574
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggagggac cgcgttcctc tacgcacgtg ccattagtct tgcccctgct
       61 agtgctttta ttactagcac cggccaggca ggccgccgcc gccccttgag accagaccaa
      121 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      181 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      241 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      301 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      361 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      421 ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat aaacccttta
      481 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      541 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      601 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      661 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      721 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      781 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
      841 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
      901 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
      961 aagttggaac ctcttacgtg cccgatcaat catgaccaaa atcccttaac gtgagttttc
     1021 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1081 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1141 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1201 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1261 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1321 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1381 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1441 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1501 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1561 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1621 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1681 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1741 tgtggataac cgtag
//
