LOCUS       sequence-45171-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, 
            Howarth M
  TITLE     Peptide tag forming a rapid covalent bond to a protein, through 
            engineering a bacterial adhesin.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 
            24.
  PUBMED    22366317
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-20"; volume: "109(12)"; pages: 
            "E690-7. Epub 2012 Feb 24"
COMMENT     Sequence Label: pDEST14-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(89..427)
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVETAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             complement(434..454)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(476..493)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             513..535
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(566..585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(567..585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga cggagctcga
       61 attcggatcc tcaagcatca accatttaaa tatgagcgtc acctttagtt gctttgccat
      121 ttacagtaac ctgaccttgc tcattaactg taaaggtaat agcagttgct acctcataac
      181 cgtctggtgc tgcggtttcg acaaatgtat attttcctgg atacaggtag aaatctttca
      241 cttgtccatc tgaaatccat gtactaatag ttttaccaga tgaatcacgc aactccatag
      301 ttgcaccagc taactctttg ccgtcctcat cacgttttga gaatttaata tgggtagcac
      361 tatcttcttc aattgtcata tcaccggact gaccttgctc acttgataaa cctgataagg
      421 tatcaaccat ggcgccctga aaatacaggt tttcggtcgt tgggatgtcg taatcgtgat
      481 ggtgatggtg atggtagtac gacatatgta tatctccttc ttaaagttaa acaaaattat
      541 ttctagaggg aaaccgttgt ggtctcccta tagtgagtcg tattaatttc gcgggatcga
      601 gatctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
      661 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
      721 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
      781 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat
      841 gttccggatc tgcatcgcag gatgctgctg gctaccctgt gg
//
