LOCUS       sequence-45172-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, 
            Howarth M
  TITLE     Peptide tag forming a rapid covalent bond to a protein, through 
            engineering a bacterial adhesin.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 
            24.
  PUBMED    22366317
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-03-20"; volume: "109(12)"; pages: 
            "E690-7. Epub 2012 Feb 24"
COMMENT     Sequence Label: pDEST14-SpyCatcher EQ
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(97..435)
                     /codon_start=1
                     /product="truncated version of the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyCatcher
                     /note="forms a covalent isopeptide bond with the SpyTag 
                     peptide (Zakeri et al., 2012)"
                     /translation="VDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDEDGKELAGATME
                     LRDSSGKTISTWISDGQVKDFYLYPGKYTFVQTAAPDGYEVATAITFTVNEQGQVTVNG
                     KATKGDAHI"
     CDS             complement(442..462)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(484..501)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             521..543
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(574..593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(575..593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttgtcgacg
       61 gagctcgaat tcggatcctc aagcatcaac catttaaata tgagcgtcac ctttagttgc
      121 tttgccattt acagtaacct gaccttgctc attaactgta aaggtaatag cagttgctac
      181 ctcataaccg tctggtgctg cggtttggac aaatgtatat tttcctggat acaggtagaa
      241 atctttcact tgtccatctg aaatccatgt actaatagtt ttaccagatg aatcacgcaa
      301 ctccatagtt gcaccagcta actctttgcc gtcctcatca cgttttgaga atttaatatg
      361 ggtagcacta tcttcttcaa ttgtcatatc accggactga ccttgctcac ttgataaacc
      421 tgataaggta tcaaccatgg cgccctgaaa atacaggttt tcggtcgttg ggatgtcgta
      481 atcgtgatgg tgatggtgat ggtagtacga catatgtata tctccttctt aaagttaaac
      541 aaaattattt ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc
      601 gggatcgaga tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg
      661 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
      721 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
      781 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcga
//
