LOCUS       sequence-451739-        3738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For gateway cloning of DXO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, 
            Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL
  TITLE     Protein and RNA ADP-ribosylation detection is influenced by sample 
            preparation and reagents used.
  JOURNAL   Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 
            10.26508/lsa.202201455.
  PUBMED    36368907
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2022-11-11"; volume: "6(1):e202201455. doi"; 
            pages: " 10.26508/lsa.202201455"
COMMENT     Sequence Label: pDONR221-DXO
FEATURES             Location/Qualifiers
     source          1..3738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..48
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     40..57
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     41..57
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    73..172
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1367..1466)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1483..1502)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1484..1502)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1507..1523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1507..1523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1625..1644)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1636..2445
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1703..1722)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2592..3180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3081..3100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3334..3351
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3510..3537)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3629..3715)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
       61 aagctcgggc cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
      121 attgatgagc aatgcttttt tataatgcca actttgtaca aaaaagcagg cttcatggat
      181 cccaggggga ccaagagagg agctgagaag acagaggtag ctgagcctcg gaacaaacta
      241 cctcgtccag caccttctct gcccacagac cctgccctct actctgggcc ctttcctttc
      301 taccggcgcc cttcggaact gggctgcttc tccctggatg ctcaacgcca gtaccatgga
      361 gatgcccgag ccctgcgcta ctatagccca ccccccacta acggtccagg ccccaacttt
      421 gacctcagag acggataccc ggatcgatac cagccccggg acgaggaggt ccaggaaagg
      481 ctggaccacc tgctgtgctg gctcctggaa caccgaggcc ggttggaggg gggtccaggc
      541 tggctggcag aggccatagt gacgtggcgg gggcacctga caaaactgct gacgacaccg
      601 tatgagcggc aggagggctg gcagctggca gcctcccggt tccagggaac actatacctg
      661 agtgaagtgg agacaccgaa cgctcgggcc cagaggcttg ctcggccacc gctcctccgg
      721 gagcttatgt acatgggata caaatttgag cagtacatgt gtgcagacaa acctggaagc
      781 tccccagacc cctctgggga ggttaacacc aacgtggcct tctgctctgt gctacgcagc
      841 cgcctgggaa gccaccctct gctcttctca ggggaggtag actgcacaga cccccaagcc
      901 ccatccacac agcccccaac ctgctatgtg gagctcaaga cctccaagga gatgcacagc
      961 cctggccaat ggaggagttt ctacagacac aagctcctga aatggtgggc tcagtcattc
     1021 ctcccagggg tcccgaatgt tgttgctggc ttccgtaacc cagacggttt tgtctcttcc
     1081 ctcaagacct ttcctaccat gaagatgttt gaatatgtca ggaatgaccg tgacggctgg
     1141 aatccctctg tgtgcatgaa cttctgtgcc gccttcctta gctttgccca gagcacggtt
     1201 gtccaggatg accccaggct cgttcatctc ttctcttggg agcctggcgg cccagtcacc
     1261 gtgtctgtac accaagatgc accttacgcc ttcctgccca tatggtatgt ggaagctatg
     1321 actcaggacc tcccatcacc ccccaagact ccctctccca aataggaccc agctttcttg
     1381 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1441 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1501 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1561 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     1621 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1681 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1741 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1801 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1861 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1921 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     1981 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2041 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2101 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2161 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2221 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2281 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2341 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2401 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2461 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2521 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2581 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2641 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2701 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2761 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2821 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2881 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2941 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3001 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3061 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3121 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3181 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3241 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3301 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3361 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3421 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3481 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3541 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3601 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3661 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3721 tggcagttcc ctactctc
//
