LOCUS       sequence-45175-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Naughton BJ, Han DD, Gu HH
  TITLE     Fluorescence-based evaluation of shRNA efficacy.
  JOURNAL   Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13.
  PUBMED    21726522
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2011-10-1"; volume: "417(1)"; pages: "162-4. Epub 
            2011 Jun 13"
COMMENT     Sequence Label: pGFPns-reporter
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(266..287)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     615..634
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agggccctct agatgcatgc tcgagcggcc gccagtgtga tggatatctg cagaattcca
       61 gcacactggc ggccgttact agtggatccg agctcggtac ctacacattg atcctagcag
      121 aagcacaggc tgcagggtga cggtccatcc cgctctcctg ggcacaagac atgggcagcg
      181 tgccatcatc ctgcgccgcc accgccggcg ggaagccatg gctcttgtac agctcgtcca
      241 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
      301 cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca
      361 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
      421 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
      481 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
      541 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      601 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      661 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      721 ggctgaagca ctgcacgccg tagctcaggg tggtcacgag ggtgggccag ggcacgggca
      781 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
      841 cctcgccgga
//
