LOCUS       sequence-45178-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo 
            X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, 
            Pei D
  TITLE     MicroRNA cluster 302-367 enhances somatic cell reprogramming by 
            accelerating a mesenchymal-to-epithelial transition.
  JOURNAL   J Biol Chem. 2011 May 13. 286(19):17359-64.
  PUBMED    21454525
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 May 13. 286(19):17359-64."
COMMENT     Sequence Label: pMXs-B1
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             520..543
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    550..583
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtggaccatc ctctagactg ccggatctag ctagttaatt aaggatccgt taacaagctt
       61 cggagaggga gtccaaaatc ctcttactct taagagtcag acattctgag attgccagtg
      121 ttattcatgt ttaagtctgt gtaaataatt gagcttatta atgtttgaag taacacaacc
      181 ccatttgcac tcttaacgga aggcaaactt gttttagaaa tagtaactca cagttcactt
      241 acttgctgtg tgtgtgtgtg tgtgtttatc caggtgagtc tgcagatctg gcaaagaaga
      301 gttcgtggaa gacttcaagg ttacactcct ggagtatgcc ttggccatgt caaagtgcta
      361 acagtgcagg tagctttttg agttctactg cagtgccagc acttcttaca ttaccatggt
      421 gatttaatca gaggccgctg agtcccctgg tttctgcata gttagattgt ggatgttttt
      481 gagctgctcc ttgtaatgtg tgaattcgta tacactagtg actacaagga cgacgatgac
      541 aagtgataaa taacttcgta taatgtatgc tatacgaagt tattctagta tttaaatgcc
      601 ctcgagctga ctgcggccgc cagcacagtg gtcgacgata aaataaaaga ttttatttag
      661 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
      721 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
      781 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
      841 ctgccccggc tcagggccaa gaacagatgg aacagctgaa
//
