LOCUS       sequence-451795-        1842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged 
            with a signal peptide and a GPI attachment site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Karapidaki I, Handberg-Thorsager M, Momose T, Yasuo H, Genikhovich 
            G, Assaf S, Deleau C, Pang Y, Clayton P, Lohmann B, Rusciano ML, 
            Stranges M, Mathieu J, Zilliox M, Ustyantsev K, Salmon B, 
            Laplace-Builhe B, Koenig M, Colgren JJ, Arnone MI, Berezikov E, 
            Brunet T, Bucher G, Burkhardt P, Dickinson DJ, Houliston E, Huisken 
            J, Leclere L, Averof M
  TITLE     A toolkit for testing membrane-localising tags across species
  JOURNAL   bioRxiv 2024.11.12.623055
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.12.623055"
COMMENT     Sequence Label: 11_T7-SP-mScarlet3-GPI
FEATURES             Location/Qualifiers
     source          1..1842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     87..108
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             270..956
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     668..687
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 taatacgact cactataggg cgaattgggt aaagtgcaag ttaaagtgaa tcaattaaaa
       61 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
      121 aatacaagaa gagaactctg ggcgccacaa ccatggagct cttttggagt atagtcttta
      181 ctgtcctcct gagtttctcc tgccgggggt cagactggga atctctgcag tcgacggtac
      241 cgcgggcccg ggatccaccg gtcgccacca tggatagcac cgaggcagtg atcaaggagt
      301 tcatgcggtt caaggtgcac atggagggct ccatgaacgg ccacgagttc gagatcgagg
      361 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgagg gtgaccaagg
      421 gtggccccct gcccttctcc tgggacatcc tgtcccctca gttcatgtac ggctccaggg
      481 ccttcacgaa gcaccccgcc gacatccccg actactggaa gcagtccttc cccgagggct
      541 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgc tgtgtccgtg gcccaggaca
      601 cctccctgga ggacggcacc ctgatctaca aggtgaagct ccgcggcacc aacttccctc
      661 ctgacggccc cgtaatgcag aagaagacaa tgggctggga agcatccacc gagcggttgt
      721 accccgagga cgtcgtgctg aagggcgaca ttaagatggc cctgcgcctg aaggacggcg
      781 gtcgctacct ggcggacttc aagaccacct acagggccaa gaagcccgtg cagatgcccg
      841 gcgctttcaa catcgaccgc aagttggaca tcacatccca caacgaggac tacaccgtgg
      901 tggaacagta cgaacgctcc gtggcccgcc actccaccgg cggctccggt ggctcctatg
      961 gaggaaacgg ctccggacag caccagtatg atccgcgccc aagcagcggt cattctagac
     1021 acagatatgc acttataccc ataccattag cagtaattac aacatgtatt gtgctgtata
     1081 tgaatgttct ttaagcgatc gctatcgatg ttataagttc tgaaccgaga aagaggggag
     1141 aatgtagagc cgatagctgc ccaatactgc cacgccccca gcagcccagg catggaaaat
     1201 aatacagaga aaatccacac ttattaggtg ctactgtaat tttttttaaa tcgcttaaaa
     1261 ttattcggcg tttgcctagt gcaaggccca tttatggaca attgtttgcc aaactcaaag
     1321 tttgccggtc ggaggaaaag ttacaacgcc aaaatgtgtt gaaaaatgca aaagaaatta
     1381 ttaaaaacat taaaaaatta ttaaataatc tatgtaattc ttgaatgtaa actctgtgta
     1441 aatactaaaa atgaaaaaca aaatggaaag cgtagatgga gaacagttga atcgttatat
     1501 atttaaatta atattgcatt tatttccaaa ttacagtggg agaagcgctg accgacttct
     1561 tgcagtgtac tctcgaaata aaacccctgt ctgtaccacg taaaacccct actaaaaatt
     1621 agtctcgccc gctgctcaca gttagtttag ttcaattata cgctactgtt taatttatta
     1681 actgtactta ttaatgcgcg tcatgaataa tgattgactg tcccttattt tttttttttg
     1741 ctaatttctg tattaaattt aatgatttac cataaaaata taaataaaaa tgacggtcga
     1801 gttgtaaaat taataaaaaa aaaaaacgaa tgctgcggcc gc
//
