LOCUS       sequence-451798-        1734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the secretion signal peptide sequence of the yeast 
            AWA1 gene for use in Combinatorial Golden Gate Assembly (BsaI 
            restriction sites)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J
  TITLE     High-throughput optimisation of protein secretion in yeast via an 
            engineered biosensor.
  JOURNAL   Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 
            10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13.
  PUBMED    39674781
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.tibtech.2024.11.010"; journalName: "Trends Biotechnol"; 
            date: "2025-04"; volume: "43"; issue: "4"; pages: "838-867"
COMMENT     Sequence Label: SP_G3
FEATURES             Location/Qualifiers
     source          1..1734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(99..133)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(204..863)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     778..797
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1045..1633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1534..1553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgttcaaca gattcaacaa acttcaggca gctctagctc tagtgctata
       61 tagtcagagt gcgttgggag ccccttgaga ccagaccaat aaaaaacgcc cggcggcaac
      121 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      181 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
      241 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
      301 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
      361 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
      421 ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
      481 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
      541 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
      601 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
      661 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
      721 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
      781 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
      841 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
      901 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
      961 ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1021 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1081 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1141 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1201 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1261 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1321 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1381 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1441 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1501 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1561 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1621 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1681 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtag
//
